In this paper, we review blood oxygenation level-dependent (BOLD) functional magnetic resonance imaging (fMRI) studies addressing the neural correlates of touch, thermosensation, pain and the mechanisms of their cognitive modulation in healthy human subjects. There is evidence that fMRI signal changes can be elicited in the parietal cortex by stimulation of single mechanoceptive afferent fibers at suprathreshold intensities for conscious perception. Positive linear relationships between the amplitude or the spatial extents of BOLD fMRI signal changes, stimulus intensity and the perceived touch or pain intensity have been described in different brain areas. Some recent fMRI studies addressed the role of cortical areas in somatosensory perception by comparing the time course of cortical activity evoked by different kinds of stimuli with the temporal features of touch, heat or pain perception. Moreover, parametric single-trial functional MRI designs have been adopted in order to disentangle subprocesses within the nociceptive system.
Available evidence suggest that studies that combine fMRI with psychophysical methods may provide a valuable approach for understanding complex perceptual mechanisms and top-down modulation of the somatosensory system by cognitive factors specifically related to selective attention and to anticipation. The brain networks underlying somatosensory perception are complex and highly distributed. A deeper understanding of perceptual-related brain mechanisms therefore requires new approaches suited to investigate the spatial and temporal dynamics of activation in different brain regions and their functional interaction. 相似文献
The fluorescence of Cu? in RbBr was investigated in the temperature range 20–300 K, by recording both emission and excitation spectra and by performing accurate lifetime and quantum yield measurements. The de-excitation mechanism has been interpreted in terms of the traditional Pedrini model. Nevertheless, a change of the compression conditions for Cu? at low temperature was observed, which implies two temperature regions (above and below 40 K) for the decay parameters. This occurrence is related not only to off-centre configuration but also to a very low value for the Cu? radius when compared to the Rb? one in RbBr. 相似文献
A study on urinary metabolites of methylprednisolone acetate (MPA) has been performed by liquid chromatography–electrospray
ionization–tandem mass spectrometry (LC-ESI-MS/MS) in precursor ion scanning (PIS) and neutral loss (NL) modes. Patients suffering
from joint inflammation have been treated with Depo-Medrol? (MPA marketed suspension, 40 mg) intra-articularly (IA) and after
a wash-out period, intramuscularly (IM) at the same dose. Urine samples have been collected after both the administration
routes. Metabolites were identified in PIS mode by setting the fragment ion at m/z 161 which is specific for MPA, methylprednisolone (MP), methylprednisolone hemisuccinate, and in NL mode by selecting the
losses of 54, 72, 176 and 194 Da. The MP-related structure of each target ion detected in both the MS modes was then confirmed
by MS/MS acquisitions, and by accurate mass experiments. By using this approach, 13 MPA metabolites (M1–M13) have been identified,
nine already reported in the literature and four unknown and for which the chemical structures have been proposed. No differences
in the metabolic pattern of MPA when administered IM or IA were observed. The relative abundances of metabolites compared
with the internal standard (MP-D2) were monitored by multiple reaction monitoring analysis for 19 days after both the administration
routes. 相似文献
We study the unsteady rotary motion of a sphere immersed in a Stokes fluid. The equation of motion for the sphere leads to
an integro-differential equation, and we are interested in the asymptotic behavior in time of the solution. Preparing initially
the system (sphere + fluid) as a stationary state, we prove that the angular velocity of the sphere slows down with a law
t−3/2 if no other forces than the one exerted by the fluid act on the sphere, while if the sphere is subject also to an elastic
torque the asymptotic behavior of the angular position of the sphere is t−γ, with γ = 5/2 if the initial angular velocity is zero, γ = 3/2 otherwise. This behavior is due to the memory effect of the surrounding fluid. We discuss briefly other initial preparations
of the system. 相似文献
There are many objects for which the attenuation varies significantly as they are rotated during computerized X‐ray tomography, for example plate samples. This can lead to significant ring artefacts in the subsequent tomographic reconstructions. In this paper a new method is presented that can successfully suppress such ring artefacts and is applicable to both parallel and cone‐beam geometries. Rapid correction is achieved via an analytical formula which involves only a matrix‐vector multiplication, for which the matrix is known and depends on a regularization parameter. The efficacy of the method is demonstrated for a paleontological sample (calcified shark cartilage) and a carbon–carbon composite/Ti–SiC metal matrix composite test sample. 相似文献
The study compares standard addition (SA), stable isotope dilution assay (SIDA) and multiple headspace extraction (MHE) as methods to quantify furan and 2-methyl-furan in roasted coffee with HS-SPME-GC-MS, using CAR-PDMS as fibre coating, d(4)-furan as internal standard and in-fibre internal standardization with n-undecane to check the fibre reliability. The results on about 150 samples calculated with the three quantitation approaches were all very satisfactory, with coefficient of variation (CV) versus the U.S. Food and Drug Administration (FDA) method, taken as reference, almost always below the arbitrarily-fixed limit of 15%. Furan was detected in the 1-5 ppm range, 2-methyl-furan in the 4-20 ppm range. Moreover, experimental exponential slopes (Q) and linearity (r) of both furan and 2-methyl-furan MHE regression equation on 50 samples were very similar thus making possible to use the same average Q value for all samples of the investigated set and their quantitation with a single determination. This makes this approach very rapid and competitive in-time with SA and SIDA. A non-separative method (HS-SPME-MS) was also developed in view of possible application on-line monitoring of furan and 2-methyl-furan in a pilot-plant with the aim of optimizing the roasting process to reduce these compounds to a minimum. Sampling times of 20 and 5 min were tested, the latter enabling total analysis time to be reduced to about 9 min. The results on 105 samples with both SIDA and MHE approaches were again highly satisfactory most of the samples giving a CV% versus the conventional methods below 20%. In this case too average Q values for both furan and 2-methyl-furan were used for MHE. The separative method presented very good repeatability (RSD% always below 10%) and intermediate precision over three months (RSD% always below 15%); performance were similar for the non-separative method, with repeatability (RSD%) always below 12% and intermediate precision over three months (RSD%) always below 15%. The sensitivity of both separative and non-separative methods was also very good, LOD and LOQ being in the ppb range for both furan and 2-methyl-furan, i.e. well below the amounts present in the roasted coffee samples. 相似文献
A new method for the simultaneous determination of D- and L-lactic acid in human plasma has been developed using high-performance liquid chromatography (HPLC) with fluorescence detection. This method is based on the reaction of lactic acid with (2S)-2-amino-3-methyl-1-[4-(7-nitro-benzo-2,1,3-oxadiazol-4-yl)-piperazin-1-yl]-butan-1-one (NBD-PZ-Val) in the presence of O-(7-azobenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HATU) and N-ethyldiisopropylamine (DIEA) to produce fluorescent diastereomeric derivatives that were easily monitored fluorimetrically at λ(ex)=490 nm and λ(em)=532 nm. The separation was achieved by use of a C18 analytical column (Synergy Hydro 150 mm x 3 mm i.d., 4 μm). The calibration curve was linear over the on-column concentration range of 10-200 μmol/L for D-lactic acid and 0.5-4.0 mmol/L for L-lactic acid. The sensitivity was good with a limit of detection of 5.24 μmol/L for D-lactic acid and 0.15 mmol/L for L-lactic acid. The analytical method was successfully applied to human plasma samples from normal healthy subjects. 相似文献
A layer of macrocyclic calix[4]arene derivatives has been grafted on the internal surface of the mesochannels of the ordered mesoporous SBA-15 to develop highly efficient trap for heavy transition metal (HTM) ions. To ensure the successful anchoring of calix[4]arene derivatives on the surface of SBA-15, two different types of calix[4]arene derivatives, one with one trimethoxysilane functional group and another with two trimethoxysilane functional groups have been explored. XRD, N(2) adsorption and TEM results provide strong evidence that the mesoporous structure of the supporting materials retain their long range ordering throughout the grafting process. Solid-state NMR, TG and FT-IR spectroscopy indicate that both types of calix[4]arene derivatives can be well-anchored on the surface of the wall of SBA-15. Calix[4]arene derivative with only one trimethoxysilane functional group showed high grafting efficiency compared to that with two trimethoxysilane functional groups due to the intramolecular and intermolecular polycondensation between two trimethoxysilane functional groups. The HTM ions extraction capacity in aqueous solution of macrocycle functionalized SBA-15 nanohybrides for a series of HTM ions has been studied. The obtained materials demonstrated very high HTM ions extraction capacity up to 96% for Pb(2+) in aqueous solution. 相似文献