全文获取类型
收费全文 | 336867篇 |
免费 | 3688篇 |
国内免费 | 1288篇 |
专业分类
化学 | 185568篇 |
晶体学 | 5313篇 |
力学 | 14332篇 |
综合类 | 10篇 |
数学 | 34825篇 |
物理学 | 101795篇 |
出版年
2018年 | 2267篇 |
2017年 | 2208篇 |
2016年 | 4293篇 |
2015年 | 3348篇 |
2014年 | 4556篇 |
2013年 | 14477篇 |
2012年 | 10536篇 |
2011年 | 13253篇 |
2010年 | 8484篇 |
2009年 | 8615篇 |
2008年 | 12132篇 |
2007年 | 12298篇 |
2006年 | 11686篇 |
2005年 | 10811篇 |
2004年 | 9816篇 |
2003年 | 8644篇 |
2002年 | 8445篇 |
2001年 | 10499篇 |
2000年 | 8060篇 |
1999年 | 6317篇 |
1998年 | 4876篇 |
1997年 | 4917篇 |
1996年 | 4840篇 |
1995年 | 4389篇 |
1994年 | 4206篇 |
1993年 | 3980篇 |
1992年 | 4715篇 |
1991年 | 4559篇 |
1990年 | 4247篇 |
1989年 | 4296篇 |
1988年 | 4247篇 |
1987年 | 4202篇 |
1986年 | 3931篇 |
1985年 | 5573篇 |
1984年 | 5580篇 |
1983年 | 4577篇 |
1982年 | 4912篇 |
1981年 | 4750篇 |
1980年 | 4622篇 |
1979年 | 4722篇 |
1978年 | 4994篇 |
1977年 | 4813篇 |
1976年 | 4688篇 |
1975年 | 4543篇 |
1974年 | 4379篇 |
1973年 | 4524篇 |
1972年 | 2800篇 |
1968年 | 2497篇 |
1967年 | 2731篇 |
1966年 | 2361篇 |
排序方式: 共有10000条查询结果,搜索用时 21 毫秒
51.
A signal registration strategy from micropatterned immunosensors that converts antigen-antibody binding reactions into electrochemical signals was demonstrated. An array-type micropatterned gold electrode on a silicon wafer was fabricated, containing two electrode geometries of rectangular (100 microm x 500 microm) and circular (r. 50 microm) types, exhibiting electrochemical characteristics of bulk and micro-electrodes, respectively. Ferritin was employed as a model analyte for immunosensing because it has an advantageous molecular structure for functionalization to the sensing interface, and is regarded as a general marker protein for tumors and cancer recurrence. With the fabricated and ferritin-functionalized immunosensors, biospecific interactions were performed with antiferritin antiserum and secondary antibody samples, followed by electrochemical signaling via an immunoprecipitation reaction by the label enzyme. Under the optimized affinity-surface construction steps and reaction conditions, both types of microfabricated electrodes exhibited well-defined calibration results as a function of the protein concentration in antiserum samples. Furthermore, circular-type micropatterned immunoelectrodes exhibited voltammetric characteristics of microelectrodes, which is advantageous in terms of sensor operation under a fixed potential and low signal drift during the signaling reaction compared with the bulk-type electrodes. The results support that the employed signaling method with the proposed immunosensor configuration is fit for sensor miniaturization and integration to future biomicrosystems. 相似文献
52.
Beeranahally H Doreswamy Madegowda Mahendra Hirihally C Devarajegowda Venkatesh B Devaru Sridhar M Anandalwar Javaregowda S Prasad 《Analytical sciences》2004,20(2):407-408
The title compound was extracted from a natural product and its structure was characterized by an X-ray diffraction method. It crystallizes in the tetragonal space group P41 with cell parameters a = 15.832(10)A, c = 11.622(10)A, Z = 4; the final residual factor is R1 = 0.0769. The structure has both intra and intermolecular hydrogen bonds. 相似文献
53.
H. William Bosch Sre
o D. kapin Egon Matijevi 《Colloids and surfaces. A, Physicochemical and engineering aspects》2004,250(1-3):43-50
Controlled precipitation of the diagnostic imaging agent ethyl 3,5-di(acetylamino)-2,4,6-triiodobenzoate has been used to produce fine particles of various sizes, morphologies, and degrees of crystallinity, which depended on experimental conditions. In addition, two distinct polymorphic forms of the drug have been fully characterized by single crystal X-ray diffraction studies, and evidence for a third polymorph was also observed. Some of the so prepared dry particles were coated with a thin layer of silica. 相似文献
54.
55.
Thomas H. Pate 《Linear and Multilinear Algebra》2003,51(3):263-278
If 1≤k≤n, then Cor(n,k) denotes the set of all n×n real correlation matrices of rank not exceeding k. Grone and Pierce have shown that if A∈Cor (n, n-1), then per(A)≥n/(n-1). We show that if A∈Cor(n,2), then , and that this inequality is the best possible. 相似文献
56.
I. P. Kuranova K. M. Polyakov E. A. Smirnova W. E. Höhne V. S. Lamzin R. Meijer 《Crystallography Reports》2003,48(6):953-958
Crystals of Saccharomyces cerevisiae inorganic pyrophosphatase suitable for X-ray diffraction study were grown by cocrystallization of the enzyme with cobalt chloride and imidodiphosphate. Saccharomyces cerevisiae is a metal-dependent enzyme which catalyzes hydrolysis of inorganic pyrophosphate to orthophosphate. The three-dimensional structure of this enzyme was solved by the molecular-replacement method and refined at 1.8 Å resolution to an R factor of 19.5%. Cobalt and phosphate ions were revealed in the active centers of both identical subunits (A and B) of the pyrophosphatase molecule. In subunit B, a water molecule was found between two cobalt ions. It is believed that this water molecule acts as an attacking nucleophile in the enzymatic cleavage of the pyrophosphate bond. It was demonstrated that cobalt ions and a phosphate group occupy only part of the potential binding sites (two chemically identical and crystallographically independent subunits have different binding sites). The arrangement of ligands and the structure of the nucleophile-binding site are discussed in relation to the mechanism of action of the enzyme and the nature of the metal activator. 相似文献
57.
C.H. Chen 《Journal of sound and vibration》2003,263(4):797-813
A neural-network-based method is offered to determine the flutter derivatives of section models under smooth and turbulent flows. The approach uses the observed dynamic responses to train an appropriate neural network. Subsequently, the modal parameters of the model for different mean velocities of wind flow are directly estimated using weight matrices in the neural network. The flutter derivatives can then be determined accurately. The validity of the present method is verified through numerical studies. Finally, the procedure is employed to process experimental data from an inverted-U-type section model, obtained from wind tunnel tests. 相似文献
58.
59.
Double-diffusive convection due to a cylindrical source submerged in a salt-stratified solution is numerically investigated
in this study. For proper simulation of the vortex generated around the cylinder, a computational domain with irregular shape
is employed. Flow conditions depend strongly on the thermal Rayleigh number, Ra
T
, and the buoyancy ratio, R
ρ. There are two types of onset of instability existing in the flow field. Both types are due to either the interaction of
the upward temperature gradient and downward salinity gradient or the interaction of the lateral temperature gradient and
downward salinity gradient. The onset of layer instability due to plume convection is due to the former, whereas, the onset
of layer instability of layers around the cylinder is due to the latter. Both types can be found in the flow field. The transport
mechanism of layers at the top of the basic plume belongs to former while that due to basic plume and layer around the cylinder
are the latter. The increase in Ra
T
reinforces the plume convection and reduces the layer numbers generated around the cylinder for the same buoyancy ratio.
For the same Ra
T
, the increase of R
ρ suppresses the plume convection but reinforces the layers generated around the cylinder. The profiles of local Nusselt number
reflects the heat transfer characteristics of plume convection and layered structure. The profiles of averaged Nusselt number
are between the pure conduction and natural convection modes and the variation is due to the evolution of layers.
Received on 13 September 1996 相似文献
60.