STEM Teachers' Planned and Enacted Attempts at Implementing Engineering Design‐Based Instruction

This study investigates grades 5 and 6 science, technology, engineering, and mathematics (STEM) teachers' planned and actualized engineering design‐based instruction, the instruments used to characterize their efforts, and the implications this work has for teachers' implementations of an integrated approach to STEM education. Participants included 23 STEM teachers from six schools (three rural, two suburban, and one urban). Data were gathered via lesson implementation plans and classroom observations. Teachers demonstrated strength in planning for standards‐ and engineering design‐based lessons, incorporating engineering practices within their respective implementation plans, and aligning their plans with content and design process standards. Missing from their plans was attention to science concepts and their placement, use, and application within a design task. Classroom observations indicated that the teacher participants gave priority to “front loading,” the design process by concentrating more of their instructional time on problem identification and planning and less time on testing designs, communicating performance results, and redesigning. Measures utilized in this study provided insight into the content of teachers' planning and subsequent instruction and suggest potential for capturing content planning in the context of classrooms in which teachers are attempting to integrate novel curriculum, such as the new standards for engineering practices.     

Pre-concentration of trace elements in short chain alcohols using different commercial cation exchange resins prior to inductively coupled plasma-optical emission spectrometric detection

Chelex-100, Dowex 50W-x8 and Dowex MAC-3 exchange resins were investigated for separation and pre-concentration of trace amounts of Cd, Cr, Cu, Fe, Mn, Pb, Ti and Zn in alcohols with respect to retention and desorption characteristics. Dowex 50W-x8 was found to be the best sorbent with percentages recoveries >95%. In addition, Chelex-100 appeared to be suitable for the pre-concentration of Cu, Fe and Zn, whereas Dowex MAC-3 was selective for Cu and Fe. Therefore, Dowex 50W-x8 was used for further investigations. The relative standard deviations <4% (n = 20), limits of detection and quantification were 0.1–1.2 μg L⁻¹ and 0.3–1.5 μg L⁻¹, respectively. The SPE method was validated against a certified reference material and the results were in agreement with certified values. The accuracy of the optimized method was verified by the recovery test in the spiked alcohol samples. The accuracy and spike recovery test for different metal ions were in the range 98–102% and 95–105%, respectively. The optimized method was applied to the separation and pre-concentration of metal ions in different commercial alcohol samples.     

An Interesting Rearrangement in the Cycloaddition of 1-(Methoxycarbony)Cyclopentadiene1

An attempted Diels-Alder reaction of 1-methoxycarbonylcyclopentadiene with cis-1,2-bis(phenylsulfonyl)ethylene gives a mixture of two sulfones, neither of which is the desired adduct. Instead a [1.5] sigmatropic rearrangement of the 1-methoxycarbonylcyclo-pentadiene precedes the 4 + 2 cycloaddition. Subsequent desulfonation also occurs in an unusual manner.     

On modelling the maximum workload allocation for parallel unrelated machines with setups

Consider a manufacturing process in which a group of machines (or people) perform a single operation on a number of different parts. The processing time depends on both the part and the machine. In addition, each machine requires significant setup time between processing different part types. The problem consists of obtaining a feasible allocation of parts to machines such that the makespan (i.e. greatest machine workload) is minimized. We present two equivalent 0–1 models. The first model arises by considering the assignment of individual parts to machines. It is amenable to Lagrangian decomposition techniques. The second model is more hierarchical in nature; it considers the two options of assigning an entire part type to a single machine, or of splitting the type across machines. The second model is more amenable than the first to branch-and-bound techniques. We report about our computational experience for finding lower bounds of the optimal solution by appending violated cuts and, ultimately, obtaining the optimal solution of real-life problems.     

A simultaneous analysis of the microwave,submillimeterwave, and infrared transitions between the ground and ν2 inversion-rotation levels of 15NH3

The submillimeterwave spectra of the pure inversion and inversion-rotation transitions in the ν₂ excited state (79 transitons) and the diode laser spectra of the ν₂ band (83 transitions) of ¹⁵NH₃ have been measured. A simultaneous least squares analysis has been carried out of these data together with previously published wavenumbers of the pure inversion transitions and inversion-rotation transitions in the ground state measured by the microwave and Fourier spectroscopy, and the ν₂ band transition frequencies obtained by the infrared-microwave two-photon technique. A theory of the Δk = ±3n interactions in the ground and ν₂ excited states of ammonia (?. Urban, V. ?pirko, D. Papou?ek, J. Kauppinen, S. P. Belov, L. I. Gershtein, and A. F. Krupnov, J. Mol. Spectrosc.88, 274–282 (1981)) has been used in the analysis. The “smoothed” values of the ν₂ band wavenumbers can be used for calibration purposes with better than 1 × 10^?3 cm^?1 precision.     

Synthesis, photochromic properties, and light-controlled metal complexation of a naphthopyran derivative

A light-controlled reversible binding switch based on photochromic 3H-naphtho[2,1-b]pyran is under development for studying cellular oscillatory calcium signals. The binding affinities of the closed and open forms of substituted naphthopyran 1 for Ca(2+), Mg(2+), and Sr(2+) in buffer were determined. The photochemically ring-opened form of the receptor exhibited increased affinity compared to the thermally stable closed form of the receptor. The binding affinity difference for Ca(2+) was approximately 77-fold at pH 7.6.     

Beyond the Capture of Circulating Tumor Cells: Next‐Generation Devices and Materials

Over the last decade, significant progress has been made towards the development of approaches that enable the capture of rare circulating tumor cells (CTCs) from the blood of cancer patients, a critical capability for noninvasive tumor profiling. These advances have leveraged new insights in materials chemistry and microfluidics and allowed the capture and enumeration of CTCs with unprecedented sensitivity. However, it has become increasingly clear that simply capturing and counting tumor cells launched into the bloodstream may not provide the information needed to advance our understanding of the biology of these rare cells, or to allow us to better exploit them in medicine. A variety of advances have now emerged demonstrating that more information can be extracted from CTCs with next‐generation devices and materials featuring tailored physical and chemical properties. In this Minireview, the last ten years of work in this area will be discussed, with an emphasis on the groundbreaking work of the last five years, during which the focus has moved beyond the simple capture of CTCs and gravitated towards approaches that enable in‐depth analysis.     

A Series of Collaborations between Various Pharmaceutical Companies and Regulatory Authorities Concerning the Analysis of Biomolecules Using Capillary Electrophoresis: Additional Instruments/Buffer

An international project team (including members from US, Canada and UK) was formed from a number of interested biopharmaceutical companies and regulatory authorities to conduct a cross-organisation collaboration exercise. The results of the first comparison with eight different organisations that used instruments of the same equipment model, the same reagents, and the same methodology has been reported previously [1]. This report represents the addition of other instruments using a different run buffer. The relative migration times were different, as expected, prohibiting a direct comparison between companies. The within-organisation variability was low for both relative migration time (<0.34% RSD% for all companies save one) and the peak area (<5% RSD% for all companies save one) when measuring the purity of a representative IgG sample. The apparent molecular weight of bovine serum albumin was measured with good precision (less than 10% RSD% across all companies) to the theoretical value when all data is utilized (67.5 kDa compared to 66.4 kDa). For a representative IgG sample, the three main components, IgG Light Chain, IgG Non-glycosylated Heavy Chain, and IgG Heavy Chain, could not be separated, specifically the IgG Non-glycosylated Heavy Chain and IgG Heavy Chain. When the IgG Non-glycosylated Heavy Chain and IgG Heavy Chain were combined for all organisations, the fractional peak area for the IgG Light Chain and IgG Non-glycosylated Heavy Chain + IgG Heavy Chain peak also showed excellent agreement, with less than 7.5 and 3.5% RSD%, respectively. The value of this exercise is in demonstrating the reliability of CE for the determination of apparent size of biopharmaceutical proteins. This underpins the appropriate use of such CE data in support of regulatory submissions.