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排序方式: 共有388条查询结果,搜索用时 15 毫秒
381.
382.
Dr. Soumya Jyoti Singha Roy Conor Loynd Delilah Jewel Sarah E. Canarelli Elise D. Ficaretta Quan A. Pham Prof. Dr. Eranthie Weerapana Prof. Dr. Abhishek Chatterjee 《Angewandte Chemie (International ed. in English)》2023,62(27):e202300961
We have developed a novel visible-light-catalyzed bioconjugation reaction, PhotoCLIC, that enables chemoselective attachment of diverse aromatic amine reagents onto a site-specifically installed 5-hydroxytryptophan residue (5HTP) on full-length proteins of varied complexity. The reaction uses catalytic amounts of methylene blue and blue/red light-emitting diodes (455/650 nm) for rapid site-specific protein bioconjugation. Characterization of the PhotoCLIC product reveals a unique structure formed likely through a singlet oxygen-dependent modification of 5HTP. PhotoCLIC has a wide substrate scope and its compatibility with strain-promoted azide-alkyne click reaction, enables site-specific dual-labeling of a target protein. 相似文献
383.
An operationally simple process has been developed for the synthesis of unsymmetrical amines and α-amino carbonyl derivatives in the absence of a catalyst, ligand, oxidant, or any additives. Contrary to known reductive amination methods, this protocol is amenable to substrates containing other reducible groups. This process effectively results in consecutive cleavage and formation of C−N bonds. DFT studies and Hammett analysis provide useful insight into the mechanism. The role of noncovalent interactions as a stabilizing factor have been examined in the protocol. A wide range of alkyl-bromides have been coupled efficiently with a variety of dimethyl anilines to get unsymmetric tertiary amines with yields up to 90%. This methodology was further extended to the synthesis of α-amino carbonyl derivatives with yields up to 93%. 相似文献
384.
Arianna O. Osgood Yunan Zheng Dr. Soumya Jyoti Singha Roy Dr. Nikolaos Biris Myer Hussain Conor Loynd Delilah Jewel Dr. James S. Italia Prof. Abhishek Chatterjee 《Angewandte Chemie (International ed. in English)》2023,62(19):e202219269
Site-specific incorporation of multiple distinct noncanonical amino acids (ncAAs) into proteins in mammalian cells is a promising technology, where each ncAA must be assigned to a different orthogonal aminoacyl-tRNA synthetase (aaRS)/tRNA pair that reads a distinct nonsense codon. Available pairs suppress TGA or TAA codons at a considerably lower efficiency than TAG, limiting the scope of this technology. Here we show that the E. coli tryptophanyl (EcTrp) pair is an excellent TGA-suppressor in mammalian cells, which can be combined with the three other established pairs to develop three new routes for dual-ncAA incorporation. Using these platforms, we site-specifically incorporated two different bioconjugation handles into an antibody with excellent efficiency, and subsequently labeled it with two distinct cytotoxic payloads. Additionally, we combined the EcTrp pair with other pairs to site-specifically incorporate three distinct ncAAs into a reporter protein in mammalian cells. 相似文献
385.
Deepshikha Yadav Shilpi Verma Jyoti Choudhary Harleen Kaur Pratibha Tiwari Sandeep Singh Pallavi Kushwaha Premshankar K. Dubey Pranjal Chandra Surinder P. Singh 《Electroanalysis》2023,35(4):e202200286
Folic acid (FA) deficiency is associated with several clinical conditions such as megaloblastic anemia, neuropsychiatric, and pregnancy-related syndromes, this makes FA an important metabolite to be monitored. We have fabricated an electrochemical biosensor based on gold nanoparticles decorated molybdenum disulfide nanosheets (AuNPs−MoS2NSs) nanocomposite as a transducer matrix for specific and rapid electrochemical detection of FA. Differential pulse voltammetry (DPV) studies displayed a rapid analytical response of the fabricated AuNPs−MoS2NSs/GCE sensor probe towards FA in a wide concentration range of 0.001–100 μM with a very low detection limit of 0.72±0.03 nM. The selectivity of the fabricated sensor probe has been examined in the presence of interferents such as dopamine, uric acid, ascorbic acid, glucose, and urea. The clinical potential of the fabricated biosensor was established by monitoring FA in human serum samples. The developed AuNPs−MoS2NSs/GCE sensor probe showed high reproducibility and stability, indicating its promise for FA detection in clinical settings. 相似文献
386.
387.
3D-printing has revolutionized various industries and scientific research by its substantial benefits such as fast prototyping, high accuracy, durability, and customized shapes. Fused deposition modeling has been used in the fabrication of 3D-printed nanocarbon electrodes. Utilization of these 3D-printed nanocarbon electrodes in the identification of organophosphates (OPs) such as parathion, methyl parathion, paraoxon, and fenitrothion, has not been reported. These compounds are highly toxic and used as chemical warfare agents and pesticides. Herein, we show that 3D-printing can be utilized for low-cost and diagnosis of priority nerve agents. 相似文献
388.
Harshita Shet Manisha Patel Jyoti M. Waikar Dr. Pavan M. More Dr. Yogesh S. Sanghvi Dr. Anant R. Kapdi 《化学:亚洲杂志》2023,18(1):e202201006
The dimethylamino functionality has significant importance in industrially relevant molecules and methodologies to install these efficiently are highly desirable. We report herein a highly efficient, room-temperature dimethylamination of chloroheteroarenes performed via the in-situ generation of dimethylamine using N,N-dimethylformamide (DMF) as precursor wiith a large substrate scope that includes various heteroarenes, purines as well as commercially relevant drugs such as altretamine, ampyzine and puromycin precursor. 相似文献