A definitive method for the determination of trace amounts of cobalt in biological materials by neutron activation analysis

A new, very accurate (definitive) method for the determination of trace amounts of cobalt in biological materials has been devised. The method is based on combination of neutron irradiation with quantitative and selective post-irradiation separation of cobalt from all accompanying radionuclides followed by measurement by -ray spectrometry. Column chromatography in which owing to addition of Co carrier the course of separation can be followed visually is the key element of the separation scheme. Several criteria have been formulated which must be simultaneously fulfilled in order to acknowledge the result as obtained by a definitive method. The high accuracy of the method has been demonstrated by the analysis of several certified reference materials of widely different Co contents.     

Enzyme and microbial sensors for phosphate, phenols, pesticides and peroxides

Biosensors employing a biocatalyst on a different level of integration have been developed for monitoring environmental pollution. These probes range from laboratory specimen to commercial detectors applied to analyzers. Recent developments on amperometric enzyme and microbial biosensors are presented here. A monoenzymatic bulk-type carbon electrode is described for biosensing organic hydroperoxides in aqueous solutions; peroxidase is immobilized within the electrode body and the direct electron transfer between electrode and enzyme is measured. Both, reversible and irreversible inhibitors of acetylcholinesterase have been quantified by using a kinetically controlled acetylcholine enzyme sequence electrode. The inhibitory effect of pesticides such as butoxycarboxime, dimethoate, and trichlorfon could be quantified within 6 min in molar concentrations. Different multi-enzyme electrodes have been developed for the determination of inorganic phosphate. These sensors represent examples of sequentially acting enzymes in combination with enzymatic analyte recycling. Using this type of amplification nanomolar concentrations can be measured.     

Determination of aromatic sulfonic acids in industrial waste water by ion-pair chromatography

A method for the identification and quantification of aromatic sulfonic acids in industrial waste water has been developed. The preparation of the samples comprises a clean-up step and an enrichment step, utilizing i) reversed-phase extraction and ii) ion-pair solid phase extraction. The aromatic sulfonic acids are separated by ion-pair chromatography with diode-array detection. An advantage of the developed ion-pair chromatographic method is the separation of linear alkylbenzenesulfonic acids (LAS), lignosulfonic acids and aromatic sulfonic acids in one chromatographic run. Also described is an optimized clean-up procedure for collected fractions of a preparative chromatographic run to identify monosulfonic acids with GC/MS after methylation. The detection limits for aromatic sulfonic acids in industrial waste waters are at the g/l level.     

Determination of a ternary mixture of penicillin-G sodium salt, penicillin-G procain salt and dihydrostreptomycin sulphate, by third-derivative spectrophotometry

Ternary mixtures of antibiotics, i.e. penicillin-G sodium salt, penicillin-G procain salt and dihydrostreptomycin sulphate salt, are assayed by 'zero-crossing' third-derivative spectrophotometry. Calibration plots follows Beer's law up to 40 mug/ml of penicillin-G sodium at 222.5 nm (r = 0.9997), 46 mug/ml of penicillin-G procain at 217 nm (r = 0.9999) and 36 mug/ml of dihydrostreptomycin sulphate at 211.5 nm (r = 0.9999), in the presence of one another. Detection limits at p = 0.05 level of significance were calculated to be, respectively, 0.66, 0.41 and 0.25 mug/ml. The procedure is rapid, simple, nondestructive and does not require solution of equations. The method was successfully applied for determining laboratory mixtures and commercial injections.     

Colorimetric method for determination of chlorine with 3,3',5,5'-tetramethylbenzidine

A new method is developed for the calorimetric determination in water of chlorine (free and combined) with 3,3′,5,5′-tetramethylbenzidine. The procedure proposed achieves a detection limit of 2 ng/ml and its sensitivity is greater than that of the methods used at present.     

On zeros of functions analytic in a half plane and completeness of systems of exponents

Sequences of zeros are described for functionsf, which, in the right half plane, are analytic and satisfy the condition ¦f(z)¦0(1) exp (¦z¦), 0<. A criterion of completeness of a system of exponents in a certain space of functions analytic in a half strip is established.Translated from Ukrainskii Matematicheskii Zhurnal, Vol. 46, No. 5, pp. 484–500, May, 1994.     

Inner Derivations and Primal Ideals of C*-Algebras

Let A be a C*-algebra. For a A let D(a, A) denote the innerderivation induced by a, regarded as a bounded operator on A,and let d(a, Z(A)) denote the distance of a from Z(A), the centreof A. Let K(A) be the smallest number in [0, ] such that d(a,Z(A)) K(A)||D(a, A)|| for all a A. It is shown that if A isnon-commutative and has an identity then either K(A) = , or K(A) = 1 / 3, or K(A) 1. Necessaryand sufficient conditions for these three possibilities aregiven in terms of the primitive and primal ideals of A. If Ais a quotient of an AW*-algebra then K(A) . Helly's Theorem is used to show that if A is aweakly central C*-algebra then K(A) 1.     

Magnetic field value in the weak energy release region of the solar corona

Spectrographic data from observations of the polarized weak steplike (with relatively sharp growth t 3 sec and slow decay t 10² sec) solar continuum radioemission are analyzed below. The main results are found using a sweeping spectrograph in the 8–12 GHz frequency range (sweep rate 1 sample per second and frequency resolution 120 MHz) and using the RT-22 radio telescope at the Crimean Astrophysical Observatory (angular resolution 4–6). In a series of events at the leading edge of the continuum a narrow-band frequency drifting emission termed blip-type is found. The degree of continuum circular polarization (p<10%) is low compared to that of the narrow-band component (p20%). Both components of the aforementioned events should be radiating on the second harmonic of the local plasma frequency in the context of a plasma generating mechanism. Based on the model proposed and using the observed degree of circular polarization of each component, we can estimate the strength of the magnetic field within the area of the source and the width of the angular spectrum from the radiating plasma turbulence. The results of analysis of the real microwave events are given below.Radiophysical Research Institute, Nizhnii Novgorod. Translated from Izvestiya Vysshikh Uchebnykh Zavedenii, Radiofizika, Vol. 37, No. 7, pp. 874–882, July, 1994.