Phage Displayed HBV Core Antigen with Immunogenic Activity

Hepatitis B is a major public health problem worldwide, which may lead to chronic liver diseases such as cirrhosis and hepatocellular carcinoma. The hepatitis B core antigen (HBcAg) is one of the major viral proteins, which forms the inner core of hepatitis B virus (HBV) particles. In this study, filamentous bacteriophage M13 was genetically modified to display the polypeptides of HBcAg in order to develop an alternative carrier system. HBcAg gene was inserted into the minor coat protein (pIII) gene of M13, and HBcAg was expressed on the phage surface as a whole protein. Antigenicity and immunogenicity of HBcAg were tested by immunizing BALB/c mice three times with HBcAg-displaying recombinant phages. After successful immunization, one of the mice with high antibody titer to HBcAg was selected for fusion, and four monoclonal antibodies specific for HBcAg were developed. This result showed that HBcAg-displaying recombinant bacteriophages are immunogenic and can potentially be used for the development of monoclonal antibodies.     

Synthesis,crystal structure and characterization of new transition metal compounds of bromophenols: Bis(2,4,6‐tribromophenolato) di(N‐methylimidazole)M(II) (M=Co,Cu)

Bis(2,4,6‐tribromophenolato)di(N‐methyl imidazole)M(II), where M stands for cobalt and copper metals, was synthesized via reaction of the corresponding metal sulphate and 2,4,6‐tribromophenolate in aqueous media in the presence of N‐methyl imidazole and sodium hydroxide. Although various crystallization procedures were applied only cobalt complex was obtained as single crystals. The Co(II) ion has a distorted octahedral enviroment involving two O atoms and two N atoms of the Bis(2,4,6‐tribromophenolato)di(N‐methyl imidazole) ligand. Powder x‐ray diffraction pattern of copper compound was used for cooper complex. For characterizations of complexes carbon, hydrogen and nitrogen elemental analysis, FTIR and UV spectroscopy, DSC thermal analysis and magnetic susceptibility measurements at room temperature were performed. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

New zeotype borophosphates with chiral tetrahedral topology: (H)0.5M1.25(H2O)1.5[BP2O8]·H2O (M = Co(II) and Mn(II))

Two new isostructural open‐framework zeotype transition metal borophosphate compounds, (H)_0.5M_1.25(H₂O)_1.5[BP₂O₈]·H₂O (M = Co(II) and Mn(II)) were synthesized by mild hydrothermal method. The structure of compounds were characterized by single‐crystal X‐ray diffraction which have ordered, alternating, vertex‐sharing BO₄, PO₄, and (MO₄)OM(H₂O)₂ groups with hexagonal, P 6₁ 2 2 (No 178) space group and unit cell parameters for Co a = 9.4960(6) Å, c = 15.6230(13) Å, for Mn a = 9.6547(12) Å, c = 15.791(3) Å, Z = 1 for both of them. TGA/DTA analysis, IR spectroscopy were used for characterization. Magnetic susceptibility measurements for both of the compound indicate strong antiferromagnetic interaction between metal centers. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

Sol-Gel Synthesis of Ceria-Zirconia-Based High-Entropy Oxides as High-Promotion Catalysts for the Synthesis of 1,2-Diketones from Aldehyde

Efficient Lewis-acid-catalyzed direct conversion of aldehydes to 1,2-diketones in the liquid phase was enabled by using newly designed and developed ceria–zirconia-based high-entropy oxides (HEOs) as the actual catalysts. The synergistic effect of various cations incorporated in the same oxide structure (framework) was partially responsible for the efficiency of multicationic materials compared to the corresponding single-cation oxide forms. Furthermore, a clear, linear relationship between the Lewis acidity and the catalytic activity of the HEOs was observed. Due to the developed strategy, exclusively diketone-selective, recyclable, versatile heterogeneous catalytic transformation of aldehydes can be realized under mild reaction conditions.     

Spectroscopic and crystal structure analysis of diamminebis(2,4,6‐triiodophenolato‐O) copper(II)

The crystal structure of [Cu(C₆H₂I₃O)₂(NH₃)₂] (CCDC 238896) has been determined by x‐ray diffraction. This monomeric centrosymmetric Cu(II) complex crystallizes in the monoclinic system. The CuO₂N₂ coordination sphere is trans ‐planar, [Cu–O: 1.943(5) Å and Cu‐N: 1.972(7)] with the fifth and sixth coordination sites occupied by I atoms from the phenoxide ions [Cu–I₁: 3.3552(8) Å] to form a tetragonally elongated octahedral structure for CuO₂N₂I₂ coordination. The complex molecules hold together in a one dimensional chain true [100] direction by intermolecular hydrogen bonds. Differantial scanning calorimeter, FTIR and magnetic susceptibility measurements were also performed in order to identify the title complex. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

Structure and electrochemical behaviour of Bis[N‐(4‐methylphenyl)salicylaldimine]copper(II) N,N′dimethylformamide solvate

The molecular model of mononuclear complex formed, through a reaction between N‐(4‐methylphenyl)salicylaldimine and Cu^II ion was determined using x‐ray diffraction method. The Cu^II coordination was found to have a distorted square‐planar structure and a cis coordination geometry was observed within the surrounding of the copper. The electro chemical behaviour of the mononuclear copper complex was studied with cyclic voltammetry.     

Phenolic Compounds from Scorzonera tomentosa L.

From the subaerial parts of S. tomentosa L. (Asteraceae), two new dihydroisocoumarins, compounds 1 and 2 , a new phthalide, 3 , and a new stilbene derivative, 4 , were isolated, together with four known compounds, (±)‐hydrangenol ( 5 ), (?)‐hydrangenol 4′‐O‐β‐glucoside ( 6 ), (±)‐hydramacrophyllol A ( 7 ), and (±)‐hydramacrophyllol B ( 8 ). All secondary metabolites were identified on the basis of physicochemical, spectroscopic, and mass‐spectrometric data. The known compounds 5 – 8 were isolated for the first time from this species.     

A blow up result for a fractionally damped wave equation

In this paper we prove a blow up result for solutions of the wave equation with damping of fractional order and in presence of a polynomial source. This result improves a previous result in [5]. There we showed that the classical energy is unbounded provided that the initial data are large enough.     

Spectrofluorimetric determination of nateglinide in pure and pharmaceutical preparations through derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole

A sensitive method was developed for the determination of nateglinide in pure and pharmaceutical preparations. The proposed method is based on the derivatization reaction between nateglinide and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole in borate buffer of pH 10. Fluorescent intensity of derivative was measured at 537 nm using an excitation wavelength of 464 nm. The described method was validated and the analytical parameters of linearity, limit of detection, limit of quantification, accuracy, precision (intra- and inter-day) and recovery were evaluated. The assay was linear over the concentration range of 50–500 ng/mL. The proposed method was applied to study of nateglinide in pure and pharmaceutical preparations.