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61.
Dendritic Glycopolymer as Drug Delivery System for Proteasome Inhibitor Bortezomib in a Calcium Phosphate Bone Cement: First Steps Toward a Local Therapy of Osteolytic Bone Lesions 下载免费PDF全文
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63.
Daniel Kogelnig Anna Regelsberger Anja Stojanovic Franz Jirsa Regina Krachler Bernhard K. Keppler 《Monatshefte für Chemie / Chemical Monthly》2011,253(3):769-772
Abstract
Extraction of Zn(II) from aqueous solution using polymer inclusion membranes based on poly(vinyl chloride) containing the phosphonium-based ionic liquid trihexyl(tetradecyl)phosphonium chloride (Cyphos? IL 101) is described. Zn(II) could efficiently be extracted from 5 M hydrochloric acid solution with membranes containing 30 wt% Cyphos IL 101, whereas back-extraction was successfully achieved with 1 M sulfuric acid. Prepared membranes were evaluated in terms of stability in air as well as in terms of leaching of the ionic liquid from the membranes in aqueous solution. 相似文献64.
A European interlaboratory collaborative study was conducted to validate a method for the quantitative determination of lipophilic
marine biotoxins based on high-performance liquid chromatography–tandem mass spectrometry. During this study, the diarrhetic
shellfish poisoning toxins okadaic acid, dinophysis toxin1 and 2 including their esters, the azaspiracids 1-3, pectenotoxin2,
and the yessotoxins were investigated at concentration levels near the limit of quantification and near the legal limit. Naturally
contaminated blue mussels, both raw and cooked and spiked extracts of clams and oysters were studied and results were obtained
for 16 test samples from 16 laboratories representing eight different countries. This article summarizes the study outcome
concerning validation key parameters like specificity, linearity, limit of detection, accuracy/recovery, and precision. Further,
influences of cooking of mussels before homogenization or hydrolysis on method robustness have been evaluated. 相似文献
65.
In this paper we are interested in optimizing proportional reinsurance and investment policies in a multidimensional Lévy-driven insurance model. The criterion is that of maximizing exponential utility. Solving the classical Hamilton-Jacobi-Bellman equation yields that the optimal retention level keeps a constant amount of claims regardless of time and the company’s wealth level.A special feature of our construction is to allow for dependencies of the risk reserves in different business lines. Dependence is modeled via an Archimedean Lévy copula. We derive a sufficient and necessary condition for an Archimedean Lévy generator to create a multidimensional positive Lévy copula in arbitrary dimension.Based on these results we identify structure conditions for the generator and the Lévy measure of an Archimedean Lévy copula under which an insurance company reinsures a larger fraction of claims from one business line than from another. 相似文献
66.
Three new metal-coordinating ligands, L(1), L(2), and L(3), have been prepared by appending o-, m-, and p-xylylguanidine pendants, respectively, to one of the nitrogen atoms of 1,4,7-triazacyclononane (tacn). The copper(II) complexes of these ligands are able to accelerate cleavage of the P-O bonds within the model phosphodiesters bis(p-nitrophenyl)phosphate (BNPP) and [2-(hydroxypropyl)-p-nitrophenyl]phosphate (HPNPP), as well as supercoiled pBR 322 plasmid DNA. Their reactivity toward BNPP and HPNPP is not significantly different from that of the nonguanidinylated analogues, [Cu(tacn)(OH(2))(2)](2+) and [Cu(1-benzyl-tacn)(OH(2))(2)](2+), but they cleave plasmid DNA at considerably faster rates than either of these two complexes. The complex of L(1), [Cu(L(1)H(+))(OH(2))(2)](3+), is the most active of the series, cleaving the supercoiled plasmid DNA (form I) to the relaxed circular form (form II) with a k(obs) value of (2.7 ± 0.3) × 10(-4) s(-1), which corresponds to a rate enhancement of 22- and 12-fold compared to those of [Cu(tacn)(OH(2))(2)](2+) and [Cu(1-benzyl-tacn)(OH(2))(2)](2+), respectively. Because of the relatively fast rate of plasmid DNA cleavage, an observed rate constant of (1.2 ± 0.5) × 10(-5) s(-1) for cleavage of form II DNA to form III was also able to be determined. The X-ray crystal structures of the copper(II) complexes of L(1) and L(3) show that the distorted square-pyramidal copper(II) coordination sphere is occupied by three nitrogen atoms from the tacn ring and two chloride ions. In both complexes, the protonated guanidinium pendants extend away from the metal and form hydrogen bonds with solvent molecules and counterions present in the crystal lattice. In the complex of L(1), the distance between the guanidinium group and the copper(II) center is similar to that separating the adjacent phosphodiester groups in DNA (ca. 6 ?). The overall geometry of the complex is also such that if the guanidinium group were to form charge-assisted hydrogen-bonding interactions with a phosphodiester group, a metal-bound hydroxide would be well-positioned to affect the nucleophilic attack on the neighboring phosphodiester linkage. The enhanced reactivity of the complex of L(1) at neutral pH appears to also be, in part, due to the relatively low pK(a) of 6.4 for one of the coordinated water molecules. 相似文献
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68.
Øystein Stakkestad Anja CV Larsen Anne-Katrine Kvissel Sissel Eikvar Sigurd Ørstavik Bjørn S Skålhegg 《BMC biochemistry》2011,12(1):7
Background
Protein kinase A type I (PKAI) and PKAII are expressed in most of the eukaryotic cells examined. PKA is a major receptor for cAMP and specificity is achieved partly through tissue-dependent expression and subcellular localization of subunits with different biochemical properties. In addition posttranslational modifications help fine tune PKA activity, distribution and interaction in the cell. In spite of this the functional significance of two forms of PKA in one cell has not been fully determined. Here we have tested the ability of PKAI and PKAII formed by expression of the regulatory (R) subunits RIα or RIIα in conjunction with Cα1 or Cβ2 to activate a co-transfected luciferace reporter gene, controlled by the cyclic AMP responsive element-binding protein (CREB) in vivo. 相似文献69.
70.
Loquet A Laage S Gardiennet C Elena B Emsley L Böckmann A Lesage A 《Journal of the American Chemical Society》2008,130(32):10625-10632
A two-dimensional proton-mediated carbon-carbon correlation experiment that relies on through-bond heteronuclear magnetization transfers is demonstrated in the context of solid-state NMR of proteins. This new experiment, dubbed J-CHHC by analogy to the previously developed dipolar CHHC techniques, is shown to provide selective and sensitive correlations in the methyl region of 2D spectra of crystalline organic compounds. The method is then demonstrated on a microcrystalline sample of the dimeric protein Crh (2 x 10.4 kDa). A total of 34 new proton-proton contacts involving side-chain methyl groups were observed in the J-CHHC spectrum, which had not been observed with the conventional experiment. The contacts were then used as additional distance restraints for the 3D structure determination of this microcrystalline protein. Upon addition of these new distance restraints, which are in large part located in the hydrophobic core of the protein, the root-mean-square deviation with respect to the X-ray structure of the backbone atom coordinates of the 10 best conformers of the new ensemble of structures is reduced from 1.8 to 1.1 A. 相似文献