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71.
Hashimoto R Fujitani N Takegawa Y Kurogochi M Matsushita T Naruchi K Ohyabu N Hinou H Gao XD Manri N Satake H Kaneko A Sakamoto T Nishimura S 《Chemistry (Weinheim an der Bergstrasse, Germany)》2011,17(8):2393-2404
Despite the growing importance of mucin core O-glycosylation in many biological processes including the protection of epithelial cell surfaces, the immune response, cell adhesion, inflammation, and tumorigenesis/metastasis, the regulation mechanism and conformational significance of the multiple introduction of α-GalNAc residues by UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (ppGalNAcTs) remains unclear. Here we report an efficient approach by combining MS and NMR spectroscopy that allows for the identification of O-glycosylation site(s) and the effect of O-glycosylation on the peptide backbone structures during enzymatic mucin domain assembly by using an isoform UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-T2 (ppGalNAcT2) in vitro. An electron-capture dissociation device in a linear radio-frequency quadrupole ion trap (RFQ-ECD) combined with a time-of-flight (TOF) mass spectrometer was employed for the identification of Thr/Ser residues occupied by α-GalNAc branching among multiple and potential O-glycosylation sites in the tandem repeats of human mucin glycoproteins MUC4 (Thr-Ser-Ser-Ala-Ser-Thr-Gly-His-Ala-Thr-Pro-Leu-Pro-Val-Thr-Asp) and MUC5AC (Pro-Thr-Thr-Val-Gly-Ser-Thr-Thr-Val-Gly). In the present study, O-glycosylation was initiated specifically at Thr10 in naked MUC4 peptide and additional introduction of α-GalNAc proceeded preferentially but randomly at three other Thr residues to afford densely glycosylated MUC4 containing six α-GalNAc residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15. On the contrary, O-glycosylation of naked MUC5AC peptide occurred predominantly at consecutive Thr residues and led to MUC5AC with four α-GalNAc residues at Thr2, Thr3, Thr7, and Thr8. The solution structures determined by NMR spectroscopic studies elicited that the preferential introduction of α-GalNAc at Thr10 of MUC4 stabilizes specifically a β-like extended backbone structure at this area, whereas other synthetic models with a single α-GalNAc residue at Thr1, Thr6, or Thr15 did not exhibit any converged three-dimensional structure at the proximal peptide moiety. Such conformational impact on the underlying peptides was proved to be remarkable in the glycosylation at the consecutive Thr residues of MUC5AC. 相似文献
72.
Zheng Y Hashidzume A Takashima Y Yamaguchi H Harada A 《Langmuir : the ACS journal of surfaces and colloids》2011,27(22):13790-13795
Macroscopic molecular recognition observations were realized using polyacrylamide-based gels modified with α-cyclodextrin (α-CD), β-cyclodextrin (β-CD), 1-naphthylmethyl (1Np), and 2-naphthylmethyl (2Np) moieties, which are denoted as αCD(x)-gel, βCD(x)-gel, 1Np(y)-gel, and 2Np(y)-gel, where x and y indicate the mol % of CD and Np moieties, respectively. The αCD(5)-gel did not adhere to either the 1Np(5)-gel or 2Np(5)-gel, whereas the βCD(5)-gel interacted with both to form alternating or checkered assemblies. Although the difference in the association constants of β-CD for the model polymers was small, the βCD(x)-gel successfully discriminated between 1Np(y)-gel and 2Np(y)-gel at the appropriate x and y. 相似文献
73.
Tsuda T Sakamoto T Nishimura Y Seino S Imanishi A Kuwabata S 《Chemical communications (Cambridge, England)》2012,48(13):1925-1927
Various metal nanoparticles including base metal were produced by a brief accelerated electron beam irradiation of 1-alkyl-3-methylimidazolium bis((trifluoromethyl)sulfonyl)amide room-temperature ionic liquid without a stabilizing agent, which is usually employed so as to prevent aggregation. 相似文献
74.
R Iino K Hayama H Amezawa S Sakakihara SH Kim Y Matsumono K Nishino A Yamaguchi H Noji 《Lab on a chip》2012,12(20):3923-3929
Active efflux of drugs, such as antibiotics, from a cell is one of the major mechanisms that cause multi-drug resistance in bacteria. Here we report a method to assess drug efflux activity in individual Escherichia coli cells enclosed and isolated in a directly accessible femtoliter droplet array with a fluorogenic compound. The inhibitory effect of a chemical compound on an exogenously expressed efflux pump system from pathogenic bacteria was easily detected at the single-cell level. We also present a proof-of-principle experiment to screen for the gene encoding a drug efflux pump by collecting individual droplets containing single cells in which the drug efflux activity was restored after introduction of the exogenous gene from pathogenic bacteria. Our approach will be a useful tool to screen novel pump inhibitors and efflux pump genes, and to overcome infectious diseases caused by multi-drug-resistant bacteria. 相似文献
75.
76.
Mitsui C Soeda J Miwa K Tsuji H Takeya J Nakamura E 《Journal of the American Chemical Society》2012,134(12):5448-5451
We here report naphtho[2,1-b:6,5-b']difuran derivatives as new p-type semiconductors that achieve hole mobilities of up to 3.6 cm(2) V(-1) s(-1) along with high I(on)/I(off) ratios in solution-processed single-crystal organic field-effect transistors. These features originate from the dense crystal packing and the resulting large intermolecular π-orbital overlap as well as from the small reorganization energy, all of which originate from the small radius of an oxygen atom. 相似文献
77.
Tanaka N Suto S Ishiyama H Kubota T Yamano A Shiro M Fromont J Kobayashi J 《Organic letters》2012,14(13):3498-3501
Two new structurally unique dimeric sesquiterpenoids, halichonadins K (1) and L (2), were isolated from an Okinawan marine sponge Halichondria sp. The structures of 1 and 2 were elucidated on the basis of spectroscopic analysis including a single crystal X-ray diffraction analysis and chemical conversion. Halichonadins K (1) and L (2) are homodimers of the eudesmane sesquiterpene linked with a piperidine ring through amide bonds. Halichonadin K (1) showed moderate cytotoxicity against KB cells. 相似文献
78.
Hideo Sawada Keisuke Umezawa Tomohiro Yokozeki Akihito Watanabe Takenori Otsu 《Experiments in fluids》2012,53(2):451-466
In order to understand the aerodynamic properties of Japanese arrows, several types of shafts without fletching and various types of Japanese arrows were examined in a low-speed wind tunnel equipped with the JAXA 60-cm magnetic suspension and balance system. The main component of drag acting on the shaft is viscous drag from the boundary layer. This viscous drag can be evaluated analytically. A simple new method to estimate the effects of fletching on the lift of the arrow was proposed in which we found that lift is proportional to the angle of attack of the arrow but not to the rotation speed or angle. Time history results for the lift on rotating arrows in the magnetic suspension and balance system indicate that this method effectively estimates the lift on an actual rotating arrow in flight. The lift and pitching moment were mainly generated by fletching, and the drag from the fletching was similar in magnitude to that of the arrow shaft. For Japanese bamboo arrows tested with a field point, the drag, lift, and pitching moment coefficients were evaluated to be in the ranges of 3.5?C3.7, 0.4?C0.8, and ?0.15 to ?0.25, respectively, at Reynolds numbers of an arrow in flight. 相似文献
79.
Aya Eizawa Kazuya Arashiba Akihito Egi Hiromasa Tanaka Kazunari Nakajima Kazunari Yoshizawa Yoshiaki Nishibayashi 《化学:亚洲杂志》2019,14(12):2091-2096
Molybdenum–iodide complexes bearing a PCP[1] ligand have been found to work as excellent catalysts toward ammonia formation under ambient reaction conditions among dinitrogen‐bridged dimolybdenum complexes and other molybdenum complexes bearing PNP and PCP[2] ligands. 相似文献
80.
Kiyama Akihito Endo Nanami Kawamoto Sennosuke Katsuta Chihiro Oida Kumiko Tanaka Akane Tagawa Yoshiyuki 《显形杂志》2019,22(3):449-457
Journal of Visualization - Realization of a needle-free drug delivery device can solve needle-caused problems. Recently, attention has been given to the use of a focused liquid jet instead of the... 相似文献