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Lambrechts SA Aalders MC Langeveld-Klerks DH Khayali Y Lagerberg JW 《Photochemistry and photobiology》2004,79(3):297-302
It is well established that for successful photoinactivation (PI) of gram-negative bacteria a cationic photosensitizer is required. This requirement suggests a charge-dependent interaction between the photosensitizer and the gram-negative bacterium, which may be influenced by the presence of ions in the suspending medium. The aim of the present study was to investigate the effect of cations Na+ and Ca2+ on the efficacy of the PI of the gram-negative Pseudomonas aeruginosa and the gram-positive Staphylococcus aureus. The bacteria were suspended in buffer containing either meso-tetra(N-methyl-4-pyridyl)-porphyrin or meso-mono-phenyl-tri(N-methyl-4-pyridyl)-porphyrin as photosensitizer and various concentrations of Na+ or Ca2+. The cell suspensions were exposed to a broadband light dose of 9 J/cm2. In buffer without added cations, P. aeruginosa and S. aureus were equally sensitive to PI. Addition of cations strongly decreased the sensitivity of both bacteria to PI, with the PI of P. aeruginosa being much more decreased than that of S. aureus, and Ca2+ being more effective than Na+. The decreased sensitivity was accompanied by a reduced binding of the photosensitizers to the bacteria. 相似文献
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Measurement of the branching ratio and asymmetry parameter for the Sigma +-->p gamma radiative decay
Timm S Albuquerque IF Bondar NF Carrigan R Chen D Cooper PS Lisheng D Denisov AS Dobrovolsky AV Dubbs T Endler AM Escobar CO Foucher M Golovtsov VL Gottschalk H Gouffon P Grachev VT Khanzadeev AV Kubantsev MA Kuropatkin NP Lach J Lang Pengfei Langland J Li Chengze Li Yunshan Luksys M Mahon JR McCliment E Morelos A Newsom C Pommot Maia MC Samsonov VM Schegelsky VA Shi Huanzhang Smith VJ Tang Fukun Terentyev NK Tkatch II Uvarov LN Vorobyov AA Yan Jie Wenheng Z Zheng Shuchen Zhong Yuanyuan 《Physical review D: Particles and fields》1995,51(9):4638-4660
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