Theoretical model and quantification of reflectance photometer

The surface morphology of lateral flow （LF） strip is examined by scanning electron microscope （SEM） and the diffuse reflection of porous strip with or without nanogold particles is investigated. Based on the scattering and absorption of nanogold particles, a reflectance photometer is developed for quantification of LF strip with nanogold particles as reporter. The integration of reflection optical density is to indicate the signals of test line and control line. As an example, serial dilutions of microalbunminuria （MAU） solution are used to calibrate the performance of the reflectance photometer. The dose response curve is fitted with a four-parameter logistic mathematical model for the determination of an unknown MAU concentration. The response curve spans a dynamic range of 5 to 200 μg/ml. The developed reflectance photometer can realize simple and quantitative detection of analyte on nanogold-labeled LF strip.     

精确标定光弹调制器的新方法

针对现有光弹调制器标定方法的不足,提出了一种精确标定光弹调制器的新方法。首先利用起偏器、波片、光弹调制器和检偏器构成标定光路,通过寻找探测信号基频分量的极大值进行粗略标定,使光弹调制器的峰值延迟量处在1.841rad附近。然后撤走波片形成光弹调制器的精确标定光路,在检偏器旋转90°前后获得探测信号的直流分量和二次谐波分量。最后利用这两种探测信号的直流分量和二次谐波分量精确地计算出光弹调制器的峰值延迟量。实验验证了此光弹调制器标定方法,实验结果表明其标定误差仅为0.7%。在此光弹调制器标定方法中,光弹调制器的标定精度和入射光强、电路参量无关。同时,标定要求的峰值延迟量小,非常适合于光弹调制器在其使用波长范围长波段的标定。