阻抑动力学荧光法测定痕量溴

在磷酸介质中 ,溴离子抑制溴酸钾氧化吡口罗红 B(PB) ,建立了测定痕量溴的阻抑动力学荧光分析法 ,并对反应机理作了初步探讨。在最优化实验条件下 ,方法的检出限为 0 .11μg/ L,线性范围 0 .36—4 .33μg/ L,该法应用于湖水、血清中溴的分析 ,结果令人满意     

吸收谱交迭的双组分混合液的荧光谱校正研究

荧光内滤效应以及样品池中荧光物质对激发光的吸收分布会直接影响荧光谱的强度和谱形,共同制约荧光分析法的应用。使用吸收谱及荧光谱存在交迭的双组分混合溶液,研究一种新的基于物理吸收模型的校正方法,以校正荧光内滤效应及吸收分布对荧光强度的影响。对三聚噻吩和五聚噻吩混合液的光谱研究表明,使用所述校正方法可以达到较为理想的校正效果, 误差小于5%。     

一种即时平面标定的二维视觉测量方法

在二维视觉测量过程中,测量平面与标定平面的重合度是影响测量精度的重要因素。现有的二维视觉测量方法将平面标定过程及测量过程分步进行,测量平面与标定平面存在离面位移,通常需要精密的约束装置对标定靶标及待测物进行校准操作。为简化二维视觉测量过程并抑制离面位移,提出一种即时平面标定的二维视觉测量方法,通过在待测物上直接投射标定的激光点阵,建立待测平面与成像平面的映射关系,再根据该映射关系对待测物表面的特征点进行测量计算。该方法仅需对系统参数进行一次标定,与无离面误差校正的传统二维视觉测量方法相比,提高了测量精度,简化了测量过程。     

多面转镜激光器扫频光学相干层析成像系统的全光谱重采样方法

在多面转镜激光器扫频光学相干层析成像系统中,激光器存在着输出光谱错位与扫频范围波动的问题.目前的重采样方法中,普遍利用互相关运算校正光谱错位,并进行大范围的截取,保证扫频范围的一致性,但这会导致成像信噪比与分辨率的降低.本文用马赫-曾德尔干涉仪(MZI)采集到的干涉信号对扫频范围波动的问题进行了详细的测量与分析,其中干涉信号的解缠相位曲线的非随机性和平行性,表明该类激光器输出光谱的波长分布具备一致性.在此基础上,提出了一种用最长扫频范围的MZI干涉信号,对样品干涉信号进行时域光谱对齐、然后进行一对多插值的重采样方法.实验与分析表明,该方法利用了所有的光谱信号,保证了样品干涉信号的能量利用率,能有效提高图像的信噪比与分辨率.     

高精度惯导系统重力扰动的阻尼抑制方法

重力扰动已经成为高精度长航时惯导系统的主要误差源之一。针对船用高精度惯导系统的重力扰动抑制问题,从舰船INS误差模型出发,推导了重力扰动在惯导系统中的传播特性。仿真结果表明垂线偏差将引起系统较大的舒拉振荡误差。为抑制重力扰动对系统的影响,引入常速度误差反馈阻尼网络和相位超前串联阻尼网络。分析了重力扰动在水平阻尼网络中的传递特性,实现了相应滤波器的设计。在此基础上完成了实验验证,海上试验结果表明,所引入的两种阻尼网络都能够阻尼掉重力扰动引起的舒拉振荡型导航误差,其中,相位超前串联阻尼网络效果更优,抑制率达到70%以上。     

Analysis of the induction of the myelin basic protein binding to the plasma membrane phospholipid monolayer

Myelin basic protein(MBP) is an essential structure involved in the generation of central nervous system(CNS)myelin.Myelin shape has been described as liquid crystal structure of biological membrane.The interactions of MBP with monolayers of different lipid compositions are responsible for the multi-lamellar structure and stability of myelin.In this paper,we have designed MBP-incorporated model lipid monolayers and studied the phase behavior of MBP adsorbed on the plasma membrane at the air/water interface by thermodynamic method and atomic force microscopy(AFM).By analyzing the pressure–area(π–A) and pressure–time(π–T) isotherms,univariate linear regression equation was obtained.In addition,the elastic modulus,surface pressure increase,maximal insertion pressure,and synergy factor of monolayers were detected.These parameters can be used to modulate the monolayers binding of protein,and the results show that MBP has the strongest affinity for 1,2-dipalmitoyl-sn-glycero-3-phosphoserine(DPPS) monolayer,followed by DPPC/DPPS mixed and1,2-dipalmitoyl-sn-glycero-3-phospho-choline(DPPC) monolayers via electrostatic and hydrophobic interactions.AFM images of DPPS and DPPC/DPPS mixed monolayers in the presence of MBP(5 n M) show a phase separation texture at the surface pressure of 20 m N/m and the incorporation of MBP put into the DPPC monolayers has exerted a significant effect on the domain structure.MBP is not an integral membrane protein but,due to its positive charge,interacts with the lipid head groups and stabilizes the membranes.The interaction between MBP and phospholipid membrane to determine the nervous system of the disease has a good biophysical significance and medical value.     

Image reconstruction tomography with only a method for laminar optical single Monte-Carlo simulation

Laminar optical tomography （LOT） is a new mesoscopic functional optical imaging technique. Currently, the forward problem of LOT image reconstruction is generally solved on the basis of Monte-Carlo （MC） methods. However, considering the nonlinear nature of the image reconstruction in LOT with the increasing number of source positions, methods based on MC take too much computation time. This letter develops a fast image reconstruction algorithm based on perturbation MC （pMC） for reconstructing the absorption or scattering image of a slab medium, which is suitable for LOT or other functional optical tomography system with narrow source-detector separation and dense sampling. To calculate the pMC parameters, i.e., the path length passed by a photon and the collision numbers experienced in each voxel with only one baseline MC simulation, we propose a scheme named as the trajectory translation and target voxel regression （TT＆TVR） based on the reciprocity principle. To further speed up the image reconstruction procedure, the weighted average of the pMC parameters for all survival photons is adopted and the region of interest （ROI） is extracted from the raw data to save as the prior information of the image reconstruction. The method is applied to the absorption reconstruction of the layered inhomogeneous media. Results demonstrate that the reconstructing time is less than 20 s with the X - Y section of the sample subdivided into 50 × 50 voxels, and the target size quantitativeness ratio can be obtained in a satisfying accuracy in the source-detector separations of 0.4 and 1.25 mm, respectively.     

光纤陀螺电路对陀螺温度性能影响的实验研究

针对干涉式光纤陀螺的电路在环境温度变化时影响陀螺输出的问题,设计了电路部分相关的温度实验,得到陀螺输出与电路温度变化之间的关系.根据陀螺输出和温度变化对陀螺输出进行补偿.结果显示,经补偿的陀螺零偏漂移减小为未补偿时的23%,零偏稳定性提高了7.32倍.     

大学物理课程思政教学设计与探索——以流体力学为例

通过对大学物理课程思政元素的分析与总结,以流体力学一章为例对理想流体模型、伯努利方程及其推论等知识点从“课程思政”教学设计方面进行了有益的探讨,推动了大学物理中流体力学课程思政的有效开展,为大学物理课程思政教学改革实践提供了一定的参考.     

基于Mathematica的弹簧摆内共振现象研究

利用Mathematica软件模拟弹簧摆的内共振现象,得出了弹簧摆的运动轨迹、振动曲线和能量曲线,计算机模拟的结果与实验结果相吻合.