酵母丙氨酸转移核糖核酸5′-半分子(1—35)的合成

本文报道了应用T_4RNA连接酶将酵母丙氨酸转移核糖核酸(tRNA_y~(Ala))5′-半分子中的三个寡核苷酸片段[—13(Ⅰ);14—22(Ⅱ);23—35(Ⅲ)]连接成5′-半分子的工作。由于寡核苦酸片段的纯度高,多核苷酸激酶和T_4RNA连接酶的质量好,采用连续反应的方法,简化了分离步骤,使产物的得率大大提高,二十二核苷酸的连接率是75％,三十五核苦酸的连接率是90％,以第一步反应原料为基数计算,最终产物的总得率是21％。经连接点和末端核苷酸分析,证明它的结构是正确的。将合成的5′-半分子与天然的3′-半分子在T_4RNA连接酶的催化下连接成人工半合成的完整tRNA_y~(Ala),具有接受[~3H]-丙氨酸和将[~3H]-丙氨酸转移到蛋白质中的生物活力。     

基于狭缝扫描的表面等离子体共振成像

利用表面等离子体共振技术进行电介质样品成像研究.采用高数值孔径显微物镜作为耦合元件,632.8nm He-Ne激光会聚激发金膜产生表面等离子体共振,通过狭缝光阑限制光束入射角,对金膜上的氮化硅光栅进行成像.反射光由放置在样品像方共轭面上的CCD摄像机接收,获得样品的表面等离子体共振像.通过扫描移动狭缝,得到入射角从44°至54°的扫描样品图像,从图像中提取样品各点的表面等离子体共振曲线,由计算机重构出样品的表面等离子体共振角谱灰度图.     

《化学教育》将我引向教研教改之路

十多年前,笔者精心设计的公开课"CO的性质实验"得到听课教师和专家的好评.于是,以<一氧化碳可燃性的实验改进>为题写成论文,带着试试看的心情第一次向<化学教育>投稿.     

Effects of atomic number Z on the energy distribution of hot electrons generated by femtosecond laser interaction with metallic targets

The effects of atomic number Z on the energy distribution of hot electrons generated by the interaction of 60fs, 130mJ, 800nm, and 7×10^17W/cm^2 laser pulses with metallic targets have been studied experimentally. The results show that the number and the effective temperature of hot electrons increase with the atomic number Z of metallic targets, and the temperature of hot electrons are in the range of 190-230keV, which is consistent with a scaling law of hot electrons temperature.     

Ax=b在广义正定矩阵类中的反问题求解

有许多类直接控制系统的绝对稳定性[1]涉及到这样一类线性方程组协Ax＝b的反问题：对于给定的x，b∈Rn，n阶实矩阵类Ⅱ（n），求解集Ⅰ（Ⅱ（n）；x，b）＝｛A∈Ⅱ（n）｜Ax＝b｝非空的条件．文[2]讨论了反问题Ⅰ（Ps（n）；x，b）≠（Ps（n）为正定降类）和Ⅰ（O（n）；x，b）≠（O（n）为正交阵类）的条件，文[3]进一步给出了Ⅰ（M-阵类；x，b）和Ⅰ（S-阵类；x，b）有解的条件．本文将研究这类反问题在更广的一类矩阵类─—广义正定矩阵[4，5]类中的求解，从而使这类反问题得到了较完满的解决．     

衍射微透镜列阵的研究与应用

本文研究了衍射微透镜列阵的设计与制作工艺方法，在此基础上研制出一系列衍射微透镜列阵，衍射效率均大于８５％，在深紫外、可见及红外波前传感器中得到了成功应用。     

UV photodegradation of polypropylene thick bars containing rutile-type TiO2 nanorods

Surface modified rutile-type titanium dioxide （CST） nanorods were used as a UV absorber in polypropylene （PP） thick bars in combination with the hindered amine light stabilizer （HALS） Chimassorb 944 （C944）. For all of the tested samples, the photodegradation was mainly limited in the region near the exposed surface, as proved by the carbonyl index and molecular weight. Compared with the typical HALS photostabilization system containing organic hindered phenol UV absorber Tinuvin~ 328 （T328）, the thickness of photodegradation region for PP/C944/CST was only a quarter to that for PP/C944 and PP/C944/T328, while the rates of reduction in molecular weight and increase in carbonyl index were much lower. Optical microscopic observation showed that the evolution of surface micro-cracks in PP/C944/CST was quite different from that in the other samples, while scanning electronic micrographs revealed that the depth of the micro-cracks in PP/C944/CST was much shorter than that in the others. It is therefore concluded that the protection of CST on PP thick bars is mainly attributed to the outstanding UV-shielding and cracks-blocking abilities.     

小型医用质子同步加速器的慢引出设计

 围绕北京医用质子同步加速器的方案, 研究设计了1/3整数共振的慢引出系统。利用该系统, 可获得较高的引出效率(大于95% )、较小的引出束流发射度(在静电切割器处, 积分发射度为0.15πmm·mrad)、较窄的引出动量分散(0.12% ) 以及较均匀的引出束流。