基于两相预柱的在线二维分离系统用于人肝组织中磷酸肽的规模化鉴定

将C18反相(RP)填充柱和磷酸基强阳离子交换(SCX)整体柱集成于一体的RP - SCX两相预柱,成功用于RPLC - MS/MS系统的自动进样和SCX分级,完成了磷酸肽在线多维分离平台的构建,可以减少样品的损失,极大地提高了系统的集成化水平和检测灵敏度.对人肝组织酶解液富集的磷酸肽进行规模化鉴定,在假阳率小于1％的情况下,共鉴定到3082条非重复的磷酸化肽段、3056个磷酸化位点和1332个磷酸化蛋白.对所鉴定到的磷酸化蛋白进行了功能分类和激酶分析.该实验结果对于了解蛋白质磷酸化在肝脏组织中发挥的生理学作用具有重要意义.     

“薄利多销”的统计建模与分析

针对2019年全国大学生数学建模竞赛的E题——"薄利多销"分析问题,首先介绍了问题的背景和问题的提法;然后给出了赛题的一个可行的解法,包括部分商品的成本价补充、打折力度的度量以及营业额、利润率与打折力度的相关分析与回归分析;最后对竞赛论文进行简要评述.     

Geomagnetic responses in high latitudes during the storm of July 15-16, 2000

The ionospheric equivalent currents in the high latitudes and the auroral electrojet system during the geomagnetic storm on July 15-16, 2000 are analyzed by using geomagnetic data from IMAGE chain. The large-scale vortices of equivalent currents are observed in the storm. The vortices on the dusk side of ionosphere correspond to four-celled pattern of plasma convection associated with NBZ, region I and region II field-aligned currents. Only one vortex can be found on the dawn side of ionosphere after interplanetary magnetic field (IMF) turns southward. In the initial phase of the storm, the center of eastward electrojets tends to shift equatorward. It arrives at 58.62o latitude of corrected geomagnetic coordinates (CGM). The westward electrojets are strong in the main phase. The center of westward electrojets in this period moves equatorward and may be beyond the southernmost station (56.45°) of the chain.     

特形脉冲调制中射频泄漏的影响及其补偿

模拟器件的非理想因素和电路分布参数等影响造成了特形脉冲调制时的射频泄漏;这些射频泄漏将对特形脉冲的激发轮廓产生缺陷.本文从特形脉冲的射频调制原理着手,分析了射频泄漏对选择激发轮廓的影响,并采用一种新的相位循环方法对它进行补偿.实验的结果表明这种补偿方法有一定的实用性.     

Studies on the Refolding of Egg White Lysozyme Denatured by Urea Using ＂Phase Diagram＂ Method of Fluorescence

The refolding of reduced and non-reducing egg white lysozymes in a urea solution was studied by a ＂phase diagram＂ method of fluorescence. The result showed that in the refolding of the reduced egg white lysozyme, an intermediate state of an egg white lysozyme exists at the urea concentrations in a final renaturation solution being about 4.5 mol/L, their refolding follows a three-state model; while in the refolding of the non-reducing egg white lysozyme, two intermediate states exist at the urea concentrations being separately 4.0 and 2.5 mol/L, and their refolding follows a four-state model. Through the comparison between the unfolding and refolding of an egg white lysozyme in the urea solution, it was found that both of the refolding of reduced and non-reducing egg white lysozyme molecules was irreversible to their unfolding in the urea solution. Finally, a suggested refolding was separately presented for the reduced and non-reducing egg white lysozymes in the urea solution.     

Single Crystalline SnO Nanosheets as Anode Material for Lithium Secondary Battery

Single crystalline SnO nanosheets with high aspect ratios have been synthesized by a simple solution-based chemical route aided with sonication. The dimension and thickness of the SnO nanosheets are controllable by adjusting the amount of cetyltrimethyl ammonium bromide （CTAB） added. The as-prepared SnO nanosheets have loosely packed {001 } surfaces and expanded lattice, thus rendering a high reversible capacity approaching the theoretical value of SnO as anode material in Li secondary battery.     

别洛索夫-扎鲍京斯基(B-Z)振荡反应——一个中级无机化学实验

非平衡非线性问题是自然科学领域中带有普遍性的问题。这个问题引起人们的重视只是近二十年的事,然而已取得了许多重要的进展。由于化学模型比较便于进行研究,因此人们以极大的兴趣瞩目于化学振荡的实验和理论研究。近两年来,在国内期刊上也发表了一些介绍化学振荡原理的文章,     

在线监测仪器国产化

国家发改委和国家环保总局支持的水和烟尘、烟气环境质量自动化在线监测仪器国产化项目不久前由北京邦达诚环境科技发展有限公司引进欧洲先进技术圆满完成。 近年来,我国环境保护部门通过购买国外仪器,在重点流域国控水质断面开展了水质在线监测。 国家环保总局鼓励国内企业引进国外先进技术进行国产化开发,邦达诚公司自筹资金引进了国外先进的水质和烟尘、烟气在线监测仪器制造技术,与地球物理研究所仪器厂共同合作,并结合我国水质和大气成分复杂、污染程度高、仪     

甾族化合物A环上的异构现象及NMR谱

在几个甾体生物碱中发现甾族化合物A环上存在有构象异构现象;应用NMR技术对该现象作了深入研究,还测定了互变异构体的活化自由能。这一现象的发现为甾族化合物的构象研究增添了新的内容。     

二甲氨基查耳酮修饰的β-环糊精在不同溶剂中的光物理行为

研究了不同过渡金属离子对以二甲氨基查尔酮修饰的β-环糊精在不同溶剂中的荧光猝灭问题.发现经过修饰的查尔酮在水溶液中对Cu2＋具有特征的识别功能.其猝灭速率常数比其他研究的金属离子大一个数量级以上.然而当该修饰环糊精处于非水溶剂如DMF中时Cu2＋离子特征识别功能不再存在, 而具有平面四方电子构型的Ni2＋则表现出最强的络合功能,这可能与化合物联结链中存在四个用亚乙基相联的络合位点易于生成平面四方结构有关.表明主/客体化合物在结构空间上的互补性,在它们的络合过程中具有重要意义.