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21.
For the first time we have shown here that the constituent from tectal extract (Te) which can support and promote the survival and growth of the retinal ganglion cell is a 30 kD protein. (i) Using MTT colorimetric microassay to measure the optical density for the survival and growth of the cultured retinal neuron, it was found that the optical densities for the experimental cultures with either Te, or its 10-30 kD fraction, or its ≥30 kD fraction were 2-4 times that of the control culture without Te (P<0.01). This indicated that experimental cultures were more active in growth, (ii) The retinal neurons cultured on Te Phast gels showed that large retinal neurons (>18 μm) grew only on the gel region containing the 30 kD protein. The retrograde prelabelled with horseradish peroxidase (HRP) for retinal ganglion cells indicated that these surviving neurons grown on Te Phast gel were HRP positive, i.e. retinal ganglion cells, (iii) The retinal explants cultured with 30 kD gels at a close distance of 1 mm ap 相似文献