Optimization of the mobile phase conditions for the purification ofTaql endonuclease

Summary Taql endonuclease was purified by high performance ion exchange liquid chromatography with linear gradient elution. Of the chromatographic media tested as mobile phases for the HPLC purification ofTaql endonuclease, sodium acetate (pH 5.0) and phosphate (pH 7.0) buffers were appropriate for use with cation exchange columns, and L-histidine (pH 6.0) and Tris (pH 8.0) buffers with anion exchange columns.     

A Robust Identification Technique for Time-Varying ARMA Processes Based on Variable Structure Systems Theory

In this paper, a novel method for extracting the values of the coefficients of time-varying ARMA processes is proposed. The approach discussed assumes solely that the orders of the numerator and the denominator polynomials are known. The algorithm is demonstrated to be stable in the sense of Lyapunov, furthermore, it is shown in the paper that the evolution in the parameter space takes place in a finite volume. The proposed method is cost effective and is based on the variable structure systems theory, which is well known with its robustness to uncertainties. In the simulation example, the coefficients of a second order ARMA process is extracted by the use of the algorithm presented. The results confirm the prominent features of the proposed technique.     

Generating function for rotation matrix elements

The rotation matrix elements are expressed in terms of the Jacobi, Hypergeometric, and Legendre polynomials in the literature. In this study, the generating function is presented for rotation matrix elements by using properties of Jacobi polynomials. In addition, some special values and Rodrigues' formula of rotation matrix elements are obtained by using the generating function. © 2011 Wiley Periodicals, Inc. Int J Quantum Chem, 2012