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排序方式: 共有141条查询结果,搜索用时 31 毫秒
21.
A novel two-dimensional electrophoretic system for the control of electroosmosis in capillary zone electrophoresis has been developed and evaluated for rapid separations of proteins. The system comprises uncoated and polyether-coated fused silica capillaries coupled in series. An equation relating the average electroosmotic flow velocity in the coupled capillaries to the intrinsic electroosmotic velocities of the connected segments and their corresponding lengths has been derived and verified experimentally. This approach has the advantage of enabling the electroosmotic flow to be tuned independently of the applied voltage. As a consequence, rapid protein analysis at relatively low field strength was achieved without sacrificing the high separation efficiencies obtained with surface-modified capillaries. 相似文献
22.
Ziad Omran 《Tetrahedron letters》2009,50(20):2434-130
We report herein the synthesis and the development of homo-bifunctional photo-cleavable sulfhydryl group cross-linkers that are able to react and then to photorelease two cysteines leading to the photoregulation of cross-linkers cleavage. 相似文献
23.
DeFlores LP Ganim Z Ackley SF Chung HS Tokmakoff A 《The journal of physical chemistry. B》2006,110(38):18973-18980
We investigate the influence of isotopic substitution and solvation of N-methylacetamide (NMA) on anharmonic vibrational coupling and vibrational relaxation of the amide I and amide II modes. Differences in the anharmonic potential of isotopic derivatives of NMA in D2O and DMSO-d6 are quantified by extraction of the anharmonic parameters and the transition dipole moment angles from cross-peaks in the two-dimensional infrared (2D-IR) spectra. To interpret the effects of isotopic substitution and solvent interaction on the anharmonic potential, density functional theory and potential energy distribution calculations are performed. It is shown that the origin of anharmonic variation arises from differing local mode contributions to the normal modes of the NMA isotopologues, particularly in amide II. The time domain manifestation of the coupling is the coherent exchange of excitation between amide modes seen as the quantum beats in femtosecond pump-probes. The biphasic behavior of population relaxation of the pump-probe and 2D-IR experiments can be understood by the rapid exchange of strongly coupled modes within the peptide backbone, followed by picosecond dissipation into weakly coupled modes of the bath. 相似文献
24.
25.
Heteroarenes such as (aza and oxa)borines are increasingly important as synthetic targets and reagents. We map the intramolecular cyclization of saturated heterocyclic chains through dative bonding. A related set of planar unsaturated aza-, oxa-, and fluora-rings that feature dative σ bonding enhanced by π delocalization are identified. The systems have, in general, the formulae A′(CH2)mD′ and A′(CH)mD′, where m?=?3 and 4, and A′ and D′ are acceptor and donor sites, respectively. In each case, the ring isomers, achievable via A′←D' internal coordination (in the manner of Kekulé’s ouroboros), are more stable than chains. Unsaturated aromatic rings examined herein include a oxadiborine with a hypervalent oxygen center and a dioxadiborine. They are isoelectronic with an azaborine, which was synthesized more recently, and benzene. 相似文献
26.
Cemil Aydoan Aslhan Gkaltun Adil Denizli Ziad El‐Rassi 《Journal of separation science》2019,42(5):962-979
In recent years, the use of organic polymer monolithic capillary columns in separation science has gained popularity due to the fact that they are easy to fabricate and do not require retaining frits. These materials have been applied in different fields including foods, proteomics, and pharmaceuticals. The interest in food analysis still needs to develop in order to increase the sensitivity towards micro/nano‐scale food applications for food samples of < 5 μg (e.g., foodomics). In this regard, polymer monolithic capillary columns offer great separation capability in the food analytical separation science. We review the most important applications in food analysis using polymer monolithic capillary columns. In addition, several examples of the use of capillary separation methods combined with mass spectrometry detection in food analysis are summarized. 相似文献
27.
In vitro evaluation of potential complexation between bovine insulin and bovine serum albumin
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Hayder Al‐Domi Muhammed Alzweiri Imad Hamdan Ziad Jaradat 《Biomedical chromatography : BMC》2014,28(3):428-432
The objective of this study was to examine the possible binding of bovine insulin (BI) with bovine serum albumin (BSA) to form a new potential diabetogenic irreversible complex protein. Several preparations of BSA and BI were prepared. Both capillary electrophoresis and spectrophotometric analysis were undertaken to test the possibility of complexation between BI and BSA. HPLC was used to test whether the potential complex of BI and BSA is reversible or irreversible. The optimum deviation between the real and calculated absorbances was observed at a BI/BSA ratio of 2. Moreover, the migration time of BI decreased substantially with increasing ratio of BI to BSA until it became almost constant at equal molar ratio of BI/BSA. While the majority of the 2:1 BI–BSA sample detached during the HPLC analysis, which confirms the reversible character of BI–BSA binding, the HPLC chromatogram also emphasizes the formation of an irreversible complexation between the two proteins. This study provides evidence of the formation of reversible and irreversible new BI–BSA complexes under physiological conditions. This highlights the importance of examining the possible diabetogenicity of BI–BSA complex in genetically susceptible people. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
28.
Damaj Z Cisnetti F Dupont L Henon E Policar C Guillon E 《Dalton transactions (Cambridge, England : 2003)》2008,(24):3235-3245
A new family of copper(I) complexes with "glycoligands" containing a central saccharide scaffold, with 2-picolyl ether groups or 2-picolylamine or N-imidazolylamine groups, has been prepared and characterized. For this purpose, the following tetradentate ligands have been synthesized: methyl 2,3-di-O-(2-picolyl)-alpha-D-lyxofuranoside (L1), 1,5-anhydro-2-deoxy-3,4-di-O-(2-picolyl)-d-galactitol (L2), 5-(amino-N-(2-salicyl))-5-deoxy-1,2-O-isopropylidene-3-O-(2-picolyl)-alpha-D-xylofuranose (L3), and 5-(amino-N-(2-salicyl))-5-deoxy-1,2-O-isopropylidene-3-O-(methylimidazol-2-yl)-alpha-D-xylofuranose (L4). The ligands and the complexes were characterized by elemental analysis, IR, 1H and 13C NMR spectroscopies, ESI mass spectrometry, and cyclic voltammetry. Collaterally with the experimental work, HF-DFT(B3LYP/6-31G*) computations were performed to obtain additional structural information. The Cu(I) complexes are found to be pentacoordinated. The redox properties and the O2-reactivity of the Cu(I)Ln complexes have been studied. Reactions of Cu(I) complexes with dioxygen in ethanol yield stable Cu(II) complexes as confirmed by UV-visible spectrophotometry and EPR spectroscopy. 相似文献
29.
In this second part of the series of investigations involving the postpolymerization modification of a hydroxy monolith (OHM) capillary column, the surface hydroxyl groups were reacted with epoxy biphenyl thus yielding the so‐called Biphenyl OHM capillary column. The modification involved the epoxy ring opening of the 2‐biphenylyl glycidyl ether catalyzed by BF3 and its subsequent reaction with the hydroxyl groups on the OHM precursor surface. The Biphenyl OHM capillary column thus obtained exhibited the typical reversed phase behavior by primarily hydrophobic interactions vis‐à‐vis the homologous series of alkyl benzenes and in addition by π–π interactions toward nitroalkane homologous series via their π‐electron rich nitro groups. This dual retention mechanism was very distinctly observed with a set of PAH solutes in the sense that the k values of the PAH solutes were comparable to those obtained on a more non polar stationary phase, namely the Epoxy OHM C‐16 reported in the preceding article. Other aromatic solutes showed the dual retention mechanism on the Biphenyl OHM capillary including phenols, anilines derivatives, and phenoxy acid herbicides. The Biphenyl OHM capillary exhibited good reproducibility from run‐to‐run, day‐to‐day, and column‐to‐column. 相似文献
30.