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101.
102.
Chitosan (CS) is expected to be an ideal gene carrier for its high biosafety. In this work, CS with low molecular weight were prepared through the γ-ray radiation on the acetic acid solution of CS. The CS chains were scissioned under the γ-ray radiation, and the molecular weight (MW) of CS decreased with the absorbed dose. When the absorbed dose was above 30 kGy, the molecular weight of CS decreased about an order of magnitude. The γ-ray-radiation-scissioned CS can e ectively bind with plasmid (pEGFP) through complex coacervation method, forming pEGFP/ γ-ray-radiation-scissioned CS complex particles with a size of 200-300 nm. The complex particles have good stability and little cytotoxicity. The in uitro gene transfection efficiencies of the pEGFP/ γ-ray-radiation-scissioned CS complex particles were investigated by fluorescence microscope and flow cytometry. The results showed that the gene vectors using γ-ray-radiation-scissioned CS as the carrier will possess better gene transfection efficiency than those using natural high-MW CS as the carrier. The higher the absorbed dose, the smaller the MW of CS and the better transfection efficiency of the corresponding gene vector. This work provides a green and simple method on the preparation of CS-based gene vectors with high efficiency and biosafety. 相似文献
103.
为快速准确检测球粉板表面缺陷,对球粉板表面光学特性不一致性和缺陷类型的多样性等关键问题进行了分析,以利于有效地解决关键问题;论文提出采用基于模板图片创建矫正模板来解决被测板材表面光学特性变动的均化问题、自适应萃取二值化阈值解决二值化阈值整定问题,以提高软件的执行鲁棒性;通过自定义算法实现了细线型缺陷修补,有效地提高了划痕类缺陷检测的精度和可靠性;提出了筛查模型实现了符合缺陷检测精度要求的噪点筛除;通过大量现场实验验证了本系统能够正确高效实现球粉板表面缺陷检测定位标识和面积测量功能,以及达到了目标检测精度;本系统能很好地胜任球粉板在线表面缺陷检测;实验证明检测系统非常高效精准,极大地提高了生产线自动化能力。 相似文献
104.
Jian Shen Huizhong Zeng Zhihong Wang Shengbo Lu Huidong Huang Jingsong Liu 《Applied Surface Science》2006,252(22):8018-8021
Polycrystalline Pb(Zr0.55Ti0.45)O3 thin film was deposited on Pt/Ti/SiO2/Si(1 0 0) by radio-frequency-magnetron sputtering method, and the writing of charge bits on the surface of PZT thin film was studied by Kelvin probe force microscopy. It is found that the surface potential of the negative charge bits are higher than those of the corresponding positive ones. When ferroelectric polarization switching occurs, the potential difference becomes even more remarkable. A qualitative model was proposed to explain the origin of the asymmetric charge writing. It is demonstrated that the internal field in the interface layer, which is near the ferroelectric/electrode interface in ferroelectric film, is likely to be the cause for the occurrence of this phenomenon. 相似文献
105.
The effects of the PbO volatilization, excess Pb content of PbZr0.52Ti0.48 (PZT) precursor, PbTiO3 (PT) seeding layers and annealing condition on the microstructures, surface morphologies, preferred orientation and ferroelectric properties of PbZr0.52Ti0.48 films were systematically investigated. PZT films with a variety of excess Pb (0-20%) were spin-deposited on Si(1 0 0) and Pt(1 1 1)/Ti/SiO2/Si(1 0 0) substrates by sol-gel technique. The films composition, Pb/Zr/Ti/O atom rate and Pb loss were semiquantitative analyzed by X-ray photoelectron spectrometer (XPS). When the excess Pb of PZT precursor was 10%, the Pb/Zr/Ti/O atomic rate of the fabricated films was very close to the designed rate of 1:0.52:0.48:3. The XRD and AFM investigations confirmed that PT seeding layer promoted the PZT films perovskite phase transformation and grains growth with (1 1 0) plane preferred orientation, accordingly lowered perovskite phase crystallization temperature and reduced Pb loss. The PZT films annealed in O2 flow demonstrated better microstructure and ferroelectric properties comparing with films annealed in air by double remnant polarization increase and 8% coercive field increase. The underlying mechanism was also investigated. 相似文献
106.
Nazikian R Berk HL Budny RV Burrell KH Doyle EJ Fonck RJ Gorelenkov NN Holcomb C Kramer GJ Jayakumar RJ La Haye RJ McKee GR Makowski MA Peebles WA Rhodes TL Solomon WM Strait EJ Vanzeeland MA Zeng L 《Physical review letters》2006,96(10):105006
Evidence is presented for a multitude of discrete frequency Alfvén waves in the core of magnetically confined high-temperature fusion plasmas. Multiple diagnostic instruments confirm wave excitation over a wide spatial range from the device size at the longest wavelengths down to the thermal ion Larmor radius. At the shortest scales, the poloidal wavelengths are comparable to the scale length of electrostatic drift wave turbulence. Theoretical analysis confirms a dominant interaction of the modes with particles in the thermal ion distribution traveling well below the Alfvén velocity. 相似文献
107.
智能化掺铒光纤放大器 总被引:3,自引:0,他引:3
介绍一种智能化的掺铒光纤放大器(EDFA)技术.基于掺铒光纤的光放大特性与EDFA内外部工作参量(如构成EDFA的元器件参量、泵浦激光参量、输入/输出光信号参量等)的关系,采用智能化处理技术使EDFA能按照外界条件,自动地调整自身工作状态,使之符合应用系统的需要.智能化掺铒光纤放大技术可以使EDFA的应用更灵活,既可用作前置放大,也可用作功率放大或线路放大,同时还带来一些新的特点和优越性能.实验制作的智能化EDFA可以在输入信号小到-40dBm或大到+10dBm即约50dB的宽动态范围内正常工作. 相似文献
108.
A multi-exposure of color fringes method has been developed to improve the capture speed of a conventional color CCD camera. In the method, four groups of projected fringe patterns encoded with different colors and different directions are stored in one CCD frame. Therefore the capture frequency of the conventional CCD can be improved to 200 Hz. It is available to measure the insect wings with low beating frequency, such as dragonfly, moth, or butterfly, whose beating frequency is about 30–40 Hz. We have used the method to measure the beating motion of a moth successfully. 相似文献
109.
Electronic absorption spectra and photoacoustic spectra of four copper(II)-amino acid complexes of formulas Cu(L-met)˙(1). Cu(DL-met)˙(2). Cu(L-ala)x(3) and Cu(DL-ala)˙-H2O(4)(L-met=L-methioninato;DL-met=DL-methioninato;L-ala=L-alaninato DL-ala=DL-alaninato) were recorded and the results are discussed quantitatively with respect to llgand field theory and the radical wave function of a non-free copper(II) ion. The relationship between the maximum absorption peak in PAS and magnetic properties is also discussed. Lastly, a qualitative explanation on the senquence of splitting energy in going from regular octahedral to strongly elongately and slightly tetragonally distorted octahedral around each copper(II) center is proposed. 相似文献
110.