Preparation of anti-human cardiac troponin I immunomagnetic nanoparticles and biological activity assays

Maghemite nanoparticles (MNPs) were synthesized by chemical coprecipitation and coated with meso-2,3-dimercaptosuccinic acid (HOOC-CH(SH)-CH(SH)-COOH or DMSA). The morphology and properties of the nanoparticles were characterized by TEM, XRD, Zeta Potential Analyzer and VSM. Subsequentially, the anti-human cardiac troponin I (cTnI) immunomagnetic nanoparticles (IMNPs) were prepared by grafting anti-human cTnI antibodies on the surface of DMSA-coated MNPs using the linker of EDC (1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride). The conjugation amount of the antibodies and the activity of IMNPs was evaluated by enzyme linked immunosorbent assay (ELISA) and Western blotting. The results show that the physical and chemical adsorption occurred at the same time, but the former was unstable and apt to desorb, and the maximum conjugation amount of antibody was about 96 μg on the 0.1 mg MNPs by covalent bond. The stability was also investigated, and after 300 days the antibodies on the IMNPs remained the biological activity.     

The Cohomology of Line Bundles on Non-primary Hopf Manifolds

The purpose of the present paper is to give an elementary method for the computation of the cohomology groups Hq（X,Ω^p X（L））, （0 ≤q ≤ n） of an n-dimensional non-primary Hopf manifold X with arbitrary fundamental group. We use the method of Zhou to generalize the results for primary Hopf manifolds and non-primary Hopf manifold with an Abelian fundamental group.     

RSFQ的初步研究

RSFQ应用于高速电子计算机是近年来活跃在低温超导电子学领域的新兴课题。文中从约瑟夫逊效应的等效模型出发 ,阐述了 RSFQ电路的基本基理及基于节点约瑟夫逊相位的数值模拟方法 ,并把它用于一个 RSFQ单向缓冲器的瞬态分析 ,给出了其相位和电压波形     

THE NONLINEAR SCHR?DINGER EQUATION AND THE CROSS-PHASE MODULATION IN ERBIUM-DOPED FIBER AMPLIFIERS

The nonlinear Schr$\ddot{o}$dinger equation(NLSE) in erbium-doped fiber(EDF) was obtained. The cross-phase modulation (XPM) in the erbium-doped fiber amplifiers (EDFA) was studied based on this NLSE and the rate equations. A more generalized form of the propagation equation in EDFA was obtained which included the phase shifts the EDFA induced. An analytical expression was given to the XPM in the EDFA. It was found that the XPM in the EDFA dose not change very much with the wavelength except at the neighboring wavelengths (around 1531 nm) where the absorption and emission cross-sections of the erbium ions reach their maxima, and the XPMs have opposite signs on the two sides around 1531 nm. Furthermore, it was found that the XPM increases with the increase of the length of EDF.     

用于活体人眼视网膜观察的自适应光学成像系统

利用自适应光学技术,研制了两套活体人眼视网膜高分辨力成像系统,在实时校正人眼波前误差的基础上,实现活体人眼视网膜细胞尺度的高分辨力成像。这两套系统分别采用19和37单元小型压电变形反射镜作为波前校正元件,哈特曼-夏克(Hartmann-Shack)波前传感器测量波前误差,用眼底反射的半导体激光作为波前探测的信标。在用计算机控制自适应光学系统实现人眼波前误差校正后,触发闪光灯照明视网膜,用CCD相机记录视网膜的高分辨力图像。校正后的残余波前误差的均方根值已分别小于1／6和1／10波长,相当于视网膜上成像分辨力分别为3．4μm和2．6μm,接近衍射极限。试验表明37单元系统的成像质量更好。     

油资源运移聚集并行数值模拟和分析

多层油资源运移聚集并行数值模拟,其功能是重建油气盆地的运移聚集演化史,对于油资源的评价,确度油藏位置,估计油藏贮量均具有重要的价值.从生产实际出发,提出数学模型,构造大规模精细并行计算的耦合算子分裂隐式迭代格式,设计了并行计算程序和并行计算的信息传递和交替方向网格剖分方法.并对不同的网格步长进行了并行计算和分析.对滩海地区进行精细数值模拟,计算结果在油田位置等方面和实际情况相吻合.对模型问题(非线型性耦合问题)进行数值分析,得到最佳阶L²误差估计,解决了这一计算渗流力学和石油地质的困难问题.     

A finite element parallel algorithm of the parametric variational principle for elastic contact problems

Basetl on the finite element solution of the parametric varialional principle of elastic con/del problem, a corresponding parallel algorithm has been created bv utilizing the specialities of parallel computer and the architecture of concurrent processing in this paper. In this algorithm. the parallelisms have heen realized in the processes of creation and assembly of stiffness matrix, of the static condensation, of the solution of stresses and in many other aspects. The programme of this algorithm has been realized on ELXSI-6400 parallel computer of Xi'an Jiaotong University. The results of computation show that the computational time can be saved efficiently and it is an effective parallel algorithm for the analyses of contact problems.     

一种迭代格式的有限元并行算法*

本文提出了一种求解有限元方程的迭代格式的并行算法.该方法在线性代数方程迭代解法的基础上,引进并行运算步骤;并且运用加权残数方法,通过选择适当的权函数,推导了该并行算法的有限元基本格式.该方法在西安交通大学BLXSI-6400并行计算机上程序实现.计算结果表明它能有效地提高运算速度,减少计算时间,是一种有效的求解大型结构有限元方程的并行算法.     

弹性接触问题参数变分原理的有限元并行算法*

本文基于弹性接触问题的参数变分原理的有限元解法,利用并行计算机的特性和并行处理结构,建立了相应的并行算法.该算法从刚度阵的生成和组集,静凝聚过程,求应力过程等多方面实现了并行化.该算法在西安交通大学ELXSI-6400并行计算机上程序实现,计算结果表明能有效地节省计算时间,是一种分析接触问题的有效的并行算法.     

六配位与八配位Oh对称晶场中Mn2+光谱及基态立方零场分裂研究

CaF₂晶体掺入Mn²⁺后,Mn²⁺取代了Ca²⁺,受到8个邻近的F^-所产生的八配位立方O_h对称晶场作用,Mckeever等计锋了Mn²⁺的吸收谱,其基态立方零场分裂参量α已有实验观测值。