Antiproliferation- and Apoptosis-Inducible Effects of a Novel Nitrated [6,6,6]Tricycle Derivative (SK2) on Oral Cancer Cells

The benzo-fused dioxabicyclo[3.3.1]nonane core is the central framework in several natural products. Using this core, we had developed a novel nitrated [6,6,6]tricycle-derived compound containing an n-butyloxy group, namely, SK2. The anticancer potential of SK2 was not assessed. This study aimed to determine the antiproliferative function and investigated possible mechanisms of SK2 acting on oral cancer cells. SK2 preferentially killed oral cancer cells but caused no harmful effect on non-malignant oral cells. After the SK2 exposure of oral cancer cells, cells in the sub-G1 phase accumulated. This apoptosis-like outcome of SK2 treatment was validated to be apoptosis via observing an increasing annexin V population. Mechanistically, apoptosis signalers such as pancaspase, caspases 8, caspase 9, and caspase 3 were activated by SK2 in oral cancer cells. SK2 induced oxidative-stress-associated changes. Furthermore, SK2 caused DNA damage (γH2AX and 8-hydroxy-2′-deoxyguanosine). In conclusion, a novel nitrated [6,6,6]tricycle-derived compound, SK2, exhibits a preferential antiproliferative effect on oral cancer cells, accompanied by apoptosis, oxidative stress, and DNA damage.     

电站锅炉中粉煤灰与SiC耐火材料结渣性研究

本文主要研究含Cr2O3的硅酸锆结合SiC耐火材料的抗粉煤灰侵蚀规律,将两种不同的粉煤灰平铺在SiC耐火材料上,作用温度为1250～1450 ℃,保温时间为40小时,然后用视频光学显微镜和XRD衍射图谱来确定其显微结构和物相组成,初步实验结果表明SiC质耐火材料具有较好的抗煤灰侵蚀性能.     

2005年分析化学与化学分析国际会议(Ⅱ)

本届大会的论文续报如下： （38）乌克兰Chmilenko FA等,合成的和天然的聚合物对偶氮类染料分析性能的影响;（39）乌克兰Fedenko V S,基于显色反应的花青素与金属离子络合的研究;（40）俄罗斯Finkelstein A L等。在X射线荧光光谱技术中所用的粉末状和粉浆状材料的一般描述;（41）俄罗斯Kartsova L A等,应用毛细管区带电泳技术测定绿茶浸出液中多酚对映体;     

Determination of Polycyclic Aromatic Hydrocarbons in Traditional Chinese Medicine Raw Material,Extracts, and Health Food Products

The four polycyclic aromatic hydrocarbon markers (PAH4) of benzo[a]anthracene (BaA), chrysene (Chr), benzo[b]fluoranthene (BbF), and benzo[a]pyrene (BaP) are indicators showing polycyclic aromatic hydrocarbon (PAH) contamination levels in Chinese medicine raw materials (CMRMs), extracts and health food products; Samples of herbal medicine, herbal extracts, and food supplements were extracted with n-hexane, then cleaned up sequentially on Florisil and EUPAH solid-phase extraction (SPE) columns. A gas chromatography–mass spectrometry method for the determination of four polycyclic aromatic hydrocarbon markers in Chinese medicine raw material, extracts, and health food products was established; In spiked-recovery experiments, the average recovery was about 78.6–107.6% with a precision of 2.3–10.5%. The limit of quantification (LOQ) and limit of detection (LOD) of the PAH4 markers in this method were 2.0 μg/kg and 0.7 μg/kg, respectively. When the developed method was utilized to determine PAH4 contents in 12 locally available health food products, 3 samples contained over 10.0 μg/kg BaP, and 5 samples contained over 50.0 μg/kg PAH4. The European Union (EU) limits for BaP and PAH4 are 10 and 50.0 μg/kg, respectively; therefore, more attention must be drawn to the exposure risk of BaP and PAH4 in CMRMs, their extracts, and health food products. According to the risk assessment based on the Margin of Exposure (MOE) method, it is recognized that the products mentioned in this study pose a low risk.     

Novel redox-active ionic thermotropic liquid crystalline complexes of polyelectrolyte and ferrocenyl surfactants

Redox-active polyelectrolyte-surfactant complexes(PSC) were prepared via the ionic self-assembly of sodium poly(styrenesulfonate) (PSS) and ferrocenyl surfactant,(11-ferrocenylundecyl)trimethylammonium bromide(FTMA) in aqueous solution. The PSS-FTMA complex exhibited an ordered interdigitated monolayer mesomorphous structure with the long period of d = 3.13 nm,and was in the ionic thermotropic liquid crystal SmA state at room temperature.Interestingly,in the solid complex, the ferrocenyl moieties formed H-aggregation showing an increase in theπ-π~* energy transfer of cyclopentadienes in the ferrocene moieties as known from the blue-shift in the UV spectrum.The complexes showed higher thermal stability compared with their components due to the ionic interaction.The PSS-FTMA film had a good redox reversibility,which promised to be used in electrochemical sensors.     

Effects of ultrasound on the desulfurization performance of hot coal gas over Zn-Mn-Cu supported on semi-coke sorbent prepared by high-pressure impregnation method

Zn-Mn-Cu/SC(U) sorbent was hydrothermally synthesized by ultrasound-assisted high-pressure impregnation method with semi-coke(SC)as support and the mixed solution of zinc nitrate,manganese nitrate and copper nitrate as active component precursors.The desulfurization performances of hot coal gas on the prepared sorbent at a mid-temperature of 500°C were tested in fixed-bed reactor.Morphology and pore structure of the prepared sorbent were also characterized by TEM,N2adsorption/desorption isotherms and XRD.For comparison,the sorbent of Zn-Mn-Cu/SC prepared by conventional high-pressure impregnation was also evaluated and characterized in order to study the effects of ultrasound treatment.Zn-Mn-Cu/SC(U) sorbent prepared by high-pressure impregnation under ultrasound-assisted condition showed a better desulfurization performance than Zn-Mn-Cu/SC.It could remove H2 S from 1000×10-6m3/m3 to 0.1×10-6m3/m3 at 500°C and maintained for 12.5 h with the sulfur capacity of 7.74%,in which both the breakthrough time and sulfur capacity were about 32% and 51% higher than those of Zn-Mn-Cu/SC sorbent.The introduction of ultrasound during high-pressure impregnation process greatly improved the morphology and pore structure of the sorbent.The ultrasonic treatment made particle size of active components smaller and made them more evenly disperse on semi-coke support,which provided more opportunities to contact with H2S in coal-based gases.However,there were no any difference in compositions and existing forms of active components on the Zn-Mn-Cu/SC and Zn-Mn-Cu/SC(U) sorbents.     

α-Arylation and Ring Expansion of Annulated Cyclobutanones: Stereoselective Synthesis of Functionalized Tetralones

α-Arylcyclobutanones display unique reactivity that makes them valuable synthetic intermediates and target molecules. We describe the preparation of α-aryl- and α-heteroarylcyclobutanones through a direct α-arylation reaction. Problematic fragmentations are avoided by the use of LiO^tBu, which promotes a rapid but reversible self-aldol reaction that slowly releases the enolate required for α-arylation. We also demonstrate the ring expansion of α-arylcyclobutanones, a process that is highlighted in the stereoselective synthesis of 1-methoxy coniothyrinone D.     

Towards Dynamic Control of Wettability by Using Functionalized Altitudinal Molecular Motors on Solid Surfaces

We report the synthesis of altitudinal molecular motors that contain functional groups in their rotor part. In an approach to achieve dynamic control over the properties of solid surfaces, a hydrophobic perfluorobutyl chain and a relatively hydrophilic cyano group were introduced to the rotor part of the motors. Molecular motors were attached to quartz surfaces by using interfacial 1,3‐dipolar cycloadditions. To test the effect of the functional groups on the rotary motion, photochemical and thermal isomerization studies of the motors were performed both in solution and when attached to the surface. We found that the substituents have no significant effect on the thermal and photochemical processes, and the functionalized motors preserved their rotary function both in solution and on a quartz surface. Preliminary results on the influence of the functional groups on surface wettability are also described.     

Polycarbonate nanoparticles spray coated on silicon substrate using micro-electromechanical-systems-based high-frequency ultrasonic nozzle

Mono-disperse polycarbonate (PC) nanoparticles 20 nm in diameter was spray coated on silicon substrate using a novel high-frequency ultrasonic nozzle. Specifically, Bisphenol-A polycarbonate with a molecular weight (M_w) of approximately 6.4 × 10⁴ g/mol was first dissolved in pyridine. The resulting solution was sprayed into surfactant-containing de-ionized (DI) water using a 300 kHz silicon-based multiple-Fourier horn nozzle (MFHN). As pyridine was extracted into the water, PC nanoparticles formed but remained dispersed. This suspension of PC nanoparticles was then sprayed onto a silicon substrate using a 500 kHz 3-Fourier horn nozzle. Scanning electron microscopy (SEM) of the dried substrate revealed that PC nanoparticles were spread uniformly with no aggregation.