Enhanced separation of purine and pyrimidine bases using carboxylic multiwalled carbon nanotubes as additive in capillary zone electrophoresis

This paper describes the enhanced separation of adenine (A), hypoxanthine (HX), 8-azaadenine (8-AA), thymine (T), cytosine (C), uracil (U) and guanine (G) by CZE dispersing carboxylic multiwalled carbon nanotubes (c-MWNTs) into the running buffer. The effect of important factors such as c-MWNT nanoparticle concentration, the acidity and concentration of running buffer, and separation voltage were investigated to acquire the optimum conditions. The seven purine and pyrimidine bases could be well separated within 16 min in a 35 cm effective length fused-silica capillary at a separation voltage of +8.0 kV in a 23 mM tetraborate buffer (pH 9.2) containing 8.0 x 10(-5) g/mL c-MWNTs. Under the optimal conditions, the linear ranges were of 2-250 microg/mL for A (R2 = 0.995), 3-200 microg/mL for U (R2 = 0.990) and G (R2 = 0.992), 3-250 microg/mL for T (R2 = 0.998), 2-200 microg/mL for C (R2 = 0.985) and 4-200 microg/mL for HX (R2 = 0.988) and 8-AA (R2 = 0.990). The detection limits were 0.9 microg/mL for A (S/N = 3), 2.4 microg/mL for U, 2.0 microg/mL for T, 1.5 microg/mL for C, 2.5 microg/mL for G and 3.0 microg/mL for HX and 8-AA. The proposed method was successfully applied for determining five purine and pyrimidine bases in yeast RNA.     

Transverse superresolution and focal shift with rotational tunable phase mask

This paper reports the imaging characteristics of an optical system can be modified by our designed polarization pupil mask. The novel rotational symmetric polarization pupil mask design based on combination of half-wave and quarter-wave plates is introduced for realizing the focal shift and extending focal depth of an optical system and the procedure for designing is presented. Numerical results show when an appropriate rotational symmetric polarization pupil mask is used as an apodizer in the optical imaging system, it not only can effectively achieve the continuously focal shift in a small range and extend focal depth of the optimized system, but also can evidently increase the transverse resolution of the optimized system at the genuine focal plane.     

Focusing of radially polarized beam with radial cosine phase wavefront

Radially polarized beam has gained much interest recently due to its properties and applications. In this article, the focusing properties of radially polarized beam with radial cosine phase wavefront are investigated theoretically. Results show that when the radially polarized beam with radial cosine wavefront phase is focused, the focal pattern differs considerably with frequency parameter in the cosine function term. In the high numerical aperture focusing system, focal shift occurs, and novel focal patterns evolve considerably, for instance, from only one peak to two or multiple overlapping peaks. In addition, peak intensity ratio of radially polarized component to longitudinal polarized component in the focal region fluctuates smoothly for low-frequency parameter, then drops sharply, and comes back remarkably with increasing frequency parameter. Simultaneously focal shift increases slowly, and then decreases, suddenly, focal shift sign changes that results from focal switch phenomenon, and then fluctuates.     

Simulation of an apodizer''s effect for high-density optical storage

The effect of an apodizer with two parallel taper refractive surfaces is theoretically investigated for highdensity optical storage. The apodizer may modulate an incident Gaussian beam into an annular beam. Simulation shows that with the increasing inner radius of the modulated beam, the focal spot shrinks obviously. The depolarization effect gets strong simultaneously, which induces the circular symmetry loss of the focal spot. In this process, pattern density of the orthogonal and longitudinal diffractive fields increases remarkably.     

Influence of Different Processing Treatments on the Detectability of Nucleic Acid and Protein Targets in Transgenic Soybean Meal

Influences of dry heating, wet heating, and extrusion on the degradation of DNA and protein from transgenic soybean meal (TSM) were analyzed using qualitative PCR, quantitative real-time PCR (qPCR), indirect enzyme-linked immunosorbent assay (iELISA), and Western blot. The 414-bp Lectin gene was thoroughly degraded after dry heating between 75 and 90 °C for 30 min, wet heating, and extrusion at 165 °C with 39 % moisture content. The 483-bp CP4-EPSPS gene was not detected after dry heating, wet heating, and extrusion at 120 °C with 39 % moisture content. The degradation ratios of both Lectin and CP4-EPSPS genes increased from 0.4 to 92.1 % and 6.1 to 84.0 % as temperatures rose from 90 to 165 °C. iELISA results of the extruded TSM showed that the CP4-EPSPS protein content was reduced from 4.19 to 0.54 % as temperatures rose from 90 to 150 °C and was not detectable at 165 °C. Western blot results also showed the degradation of CP4-CPSPS protein after extrusion. Our results showed that temperature played an essential role in DNA and protein degradation, and the content of genetically modified organism (GMO) products may be changed after processing and could not reflect the initial content of the products.     

A numerical study on ion concentration polarization and electric circuit performance of an electrokinetic battery

Ion concentration polarization (ICP) imposes remarkable adverse effects on the energy conversion performance of the pressure-driven electrokinetic (EK) flows through a capillary system that can be equivalently treated as a battery. An optimized dimensionless numerical method is proposed in this study to investigate the causes and the effects of the ICP. Results show that remarkable ICP phenomena are induced under certain conditions such as high applied pressure, high surface charge density, and small inversed Debye length at dimensionless values of 6000, ‒10, and 0.5. Meanwhile, different factors influence the ICP and the corresponding electric properties in different ways. Particularly for the overall electric resistance, the applied pressure and the surface charge density mainly affect the variation amplitude and the level of the overall electric resistance when varying the output electric potential, respectively. Differently, the Debye length affects the overall electric resistance in both aspects. Ultimately, the induced ICP leads to significant nonlinear current–potential curves.     

Design and construction of a static tester for blue ray optical storage

Blue ray optical storage is one of the important trends in the area of information storage, and attracts a number of researchers. Static tester for blue ray optical storage plays an important role in storage media research. We designed and constructed a static tester, in which modularization makes it very convenient to expand function. Employment of modulation/demodulation technique weakens test error so as to increase test precision. Focus move mode and the nano-positioners facilitate the relocation of recording marks. Only one laser with wavelength 406.7 nm is used. In this paper, system design and characteristics are represented in detail, and some experimental results are also given to show that the static tester can perform successfully, acting as research platform for blue ray optical storage.     

电流变液中的旋光现象

根据光波在介质中的传播理论，由麦克斯韦方程出发，对非均相结构电流变流导出在外电场与光场交互作用下旋光角ψ与入射线偏振光振动方向与外加电场方向间夹角θ及ζ（取决于电流变液材料本身及外加电场强度）的函数关系式。对不同浓度、不同场强下的SiO2电为液的旋光进行了测量，实验发现，同一场强作用下，旋光角ψ随浓度c的增大而增大；对于同一浓度，旋光角ψ随场强E增大而增大；在浓度c与场强E一定的情况下，旋光角ψ还随θ角而变化。实验结果与理论预测吻合较好。     

Construction of a Standard Reference Plasmid for Detecting GM Cottonseed Meal

A plasmid was constructed for quantification of genetically modified (GM) cottonseed meal in the gene-specific level. The Cry1Ab/c gene was connected with the Sad1 gene by fusion PCR. The fusion gene was cloned into the pMD®19-T Simple Vector. The plasmid DNA was then digested with a restriction endonuclease SmaI to reduce the characteristic differences between the plasmid DNA and genomic DNA. For a rough quantitative analysis of GM cotton meal contents, a rapid method for measurement of the copy numbers of the transgenic Cry and cotton endogenous Sad1 gene using a real-time PCR system with the plasmid DNA as a calibrator was established. The inter-run and intra-run coefficients of variation were less than 1.48% and 2.36%, respectively. The limits of detection and quantitation of the Cry and Sad1 genes were 9 and 91 copies of pMDCS, respectively. These results prove that the standard plasmid represents a valuable alternative to genomic DNA as a certified reference material for the quantification of GM cotton and is a useful tool to establish a feasible identification management for GM cottonseed meal content in the feed industry.