Detection of Bacterial Neutral Ceramidase in Diabetic Foot Ulcers with an Optimized Substrate and Chemoenzymatic Probes

Ceramidases (CDases) are important in controlling skin barrier integrity by regulating ceramide composition and affording downstream signal molecules. While the functions of epidermal CDases are known, roles of neutral CDases secreted by skin-residing microbes are undefined. Here, we developed a one-step fluorogenic substrate, S-B , for specific detection of bacterial CDase activity and inhibitor screening. We identified a non-hydrolyzable substrate mimic, C6 , as the best hit. Based on C6 , we designed a photoaffinity probe, JX-1 , which efficiently detects bacterial CDases. Using JX-1 , we identified endogenous low-abundance PaCDase in a P. aeruginosa monoculture and in a mixed skin bacteria culture. Harnessing both S-B and JX-1 , we found that CDase activity positively correlates with the relative abundance of P. aeruginosa and is negatively associated with wound area reduction in clinical diabetic foot ulcer patient samples. Overall, our study demonstrates that bacterial CDases are important regulators of skin ceramides and potentially play a role in wound healing.     

基于CAN总线的振动试验智能监控系统软件设计

振动试验是为了保障航天及民用产品可靠性的重要试验手段,传统的振动试验对功放的操作繁琐,效率低下,已经越来越无法适应新型振动试验的发展。提出一种基于CAN总线控制器构建的振动试验功率放大器远程监控系统,使用Visual C++进行编程,实现了在计算上对功率放大器的远程操作,解放了操作人员,同时由于计算机的开放性,系统可以扩展出运行参数实时显示,配置本地存储、保护互锁、开机自检等功能,最后通过实验验证表明系统软件运行可靠,达到了预期的设计目的。     

Deposition of ZnO thin films on (100) γ-LiAlO2 substrate

Optical properties for ZnO thin films grown on (100) γ-LiAlO2 (LAO) substrate by pulsed laser deposition method were investigated. The c-axis oriented ZnO films were grown on (100) γ-LiAlO2 substrates at the substrate temperature of 550 C. The transmittance of the films was over 85%. Peaks attributed to excitons were shown in absorption spectra, which indicated that thin films had high crystallinity. Photoluminescence spectra with the maximum peak at 540 nm were observed at room temperature, which seemed to be ascribed to oxygen vacancy in the ZnO films caused by diffusion of Li from the substrates into the films during the deposition.     

Field emission properties and synthesis of carbon nanotubes grown by rf plasma-enhanced chemical vapor deposition

Plasma-enhanced chemical vapor deposition (PECVD) method was employed to grow the Fe-catalyzed carbon nanotubes (CNTs). The grown CNTs with a uniform diameter in the range of about 10-20 nm and the typical lengths beyond 1 μm resulted in a very high aspect ratio. The Raman and TEM results showed that the grown CNTs contained a large amount of carbonaceous particles and crystal defects, such as pentagon-heptagon pair defects. XPS measurement indicated that the CNTs had CH covalent bonds. Field emission characteristics exhibited the low turn-on threshold field of 2.75 V/μm and the maximum emission current density of 7.75 mA/cm² at 6.5 V/μm. The growth mechanism of CNTs and the effects of hydrogen plasma on their structure were discussed.     

类铜离子的电子碰撞参数及其规律

用准相对论扭曲波方法系统地计算了Ｂａ、Ｎｄ、Ｇｄ、Ｙｂ、Ａｕ、Ｐｂ类铜离子组态能级之间的电子碰撞激发强度Ω（ｎｌ－ｎｌ），３≤ｎ≤７，４≤ｎ≤７，同时给出了高能极限的碰撞强度和外推到阈值的碰撞强度，用最小二乘样条方法拟合了全能域碰撞强度及热平均速率系数。对于一个激发过程，用１０个参数可以得到碰撞电子在任意能量下的碰撞强度以及任意温度下的速率系数。     

一种修正的HS共轭梯度法及全局收敛性

<正>1引言考虑无约束极小化问题:(?),(1)其中f(x)连续可微,其梯度函数用g(x)表示.共轭梯度法求解(1)的常用迭代格式为:x_(k+1)=x_k+α_kd_k,(2)(?)(3)其中g_k=▽f(x_k),α_k≥0是由某种线搜索得到的步长因子;d_k为搜索方向,β_k为标量,β_k的不同选择产生了不同的共轭梯度法.著名的β_k公式有:     

综合评判的神经网络方法

综合评判的方法已经广泛运用到信息，决策，管理和控制等领域，但是它的两大难点－权重和单因素评价矩阵的确定越来越来明显地展现在人们面前，本文试图用神经网络方法来解决这两个难点，并从理论上证明了这种方法的收敛性。     

Ce掺杂6H-SiC电子结构和光学性质的第一性原理研究

采用基于密度泛函理论的第一性原理计算方法,对未掺杂及Ce掺杂6H-SiC的电子结构和光学性质进行理论计算.计算结果表明,未掺杂6H-SiC是间接带隙半导体,其禁带宽度为2.045 eV,掺杂Ce元素,带隙宽度下降为0.812 eV.未掺杂6H-SiC在价带的低能区,Si-3s、C-2s电子轨道对态密度的贡献较大,在价带的高能区,主要是由Si-3p、Si-3s、C-2p态组成.掺杂后Ce原子的4f轨道主要贡献在导带部分,掺杂后电导率提高.未掺杂时,只有一个介电峰,是价带电子跃迁到导带电子所致,掺杂后有两个介电峰,第一个介电峰是由于导带电子跃迁到Ce原子4f轨道上产生,第二个峰是价带电子向导带电子跃迁产生.未掺杂6H-SiC,在能量为10.31 eV处吸收系数达到最大值,掺杂后在能量为6.57 eV处,吸收系数达到最大值.     

计算机在转动惯量实验数据处理中的应用

本文具体介绍了计算机辅助处理转动惯量实验中数据结果的应用,提出了一种方便、快速、准确地验证数据结果的方法。