铕敏化荧光法测定甲磺酸帕苏沙星

稀土Eu3+能与甲磺酸帕苏沙星形成络合物,并发出Eu3+的特征荧光.加入表面活性剂SDBS能大大增强体系的荧光强度,由此建立了表面活性剂敏化的Eu3+荧光探针测定甲磺酸帕苏沙星的方法.最佳测定条件为最大激发和发射波长分别是332和618 nm;pH=7.5;Eu3+浓度为1.2×10-5 mol/L,SDBS浓度为3.0×10-5 mol/L.甲磺酸帕苏沙星浓度在3.0×10-8～2.0×10-6 mol/L范围内与荧光强度线性关系良好,r=0.9993;检出限为1.0×10-9 mol/L.该方法用于甲磺酸帕苏沙星注射剂和人体血清以及尿样中的甲磺酸帕苏沙星的含量测定,结果令人满意.     

水/油型微乳液中球形及棒状钛酸锶钡纳米粒子的控制合成

Barium strontium titanate nanoparticles with spherical and rod-like morphologies were synthesized in water/Triton X-100/n-hexanol/cyclohexane quaternary reverse microemulsion solution. The influences of the molar ratio of water to surfactant (ω₀) and the concentration of reactants on the morphology and size of barium strontium titanate nanoparticles were studied. The structure, compositions and morphology of the prepared products were characterized by XRD, SAED, ICP, EDS and TEM. The results show that the obtained Ba_0.7Sr_0.3TiO₃ spherical nanoparticles with diameters of 20～100 nm and the Ba_0.7Sr_0.3TiO₃ nanorods with diameters of 70～120 nm and lengths up to 600～800 nm are a single crystal, with a cubic phase. The molar ratio for barium, strontium and titanate in products is about 0.7:0.3:1.     

吲哚-3-乙酸与部分过渡金属离子M(Ⅱ)二元配合物的pH电位法研究

在25℃,I=0.10 mol/L KNO3的实验条件下,用pH电位法测定了吲哚-3-乙酸(IAA)在甲醇-水混合溶剂中的质子化常数,讨论了有机溶剂对质子化常数的影响,结果表明在实验范围内溶剂甲醇含量对质子化常数的影响呈线性关系.确定了二元体系M(Ⅱ)-IAA(M=Fe、Co、Ni、Cu、Zn和Cd)在甲醇含量为50%(V/V)的混合溶剂中主要存在的配合物物种,并测定了配合物的稳定常数.实验数据的处理运用计算机程序Hyperquad、Glee和Hyss完成.     

基于支持向量机的羰基化合物红外光谱研究

设计了一个4层分等级分类系统，通过支持向量机技术对羰基化合物的红外光谱成功地进行了信息分类和提取，并与人工神经网络进行比较，结果表明：支持向量机对羰基类化合物红外光谱分类效果优于人工神经网络；另外详细探讨了表征酰胺类化合物的特征光谱峰片段对识圳伯仲叔酰胺的影响，证明了酰胺的N-H伸缩振动峰对识别伯仲叔酰胺贡献最大，为构建红外光谱智能解析系统提供了定量依据。     

Synthesis and Structural Characterization of cis- and trans-bis(4,4′-dimethyl-2,2′-bipyridine)cadmium(II) Nitrates Prepared in a One-pot Reaction

The cis and trans isomers of [Cd(dmbpy)₂](NO₃)₂] (dmbpy = 4,4′-dimethyl-2,2′-bipyridine) have been synthesized simultaneously via a one-pot reaction of Cd(NO₃)₂ · 4H₂O with the dmbpy ligand in a MeOH/H₂O mixture; they can be obtained individually by means of the mechanical separation due to their different growing habit of the single-crystals. Different π-stacking interactions are observed in the cis and trans isomers forming different one-dimensional networks. The outcome of the DFT calculations agrees well with the experimental results.     

不同水煤浆分散剂与煤之间的相互作用规律研究Ⅹ分散剂在煤粒表面上的吸附作用特征

研究了6种水煤浆分散剂在14种不同变质程度煤上的吸附作用特征。结果表明,多数分散剂在煤粒表面达到单层饱和吸附后,又形成多层吸附,单层饱和吸附量与煤的变质程度、比表面积以及分散剂的性质有关。在相同粒度分布下,煤的变质程度越低,表面含氧亲水官能团的比例越高,孔隙率越高,比表面积越大,这对增大吸附量有利。煤的变质程度越高,其表面疏水区面积的比例越高,分散剂通过疏水基团紧密吸附在煤表面的比例越大,这对增加高阶煤的吸附量有利。对不同煤,是变质程度还是比表面积为吸附分散剂的主控因素,主要依赖于分散剂的结构与性质。对同种煤,疏水与亲水基团呈线型分立分布的分散剂,吸附量明显高;而疏水与亲水基团呈线型间隔分布的分散剂,吸附量明显小。     

Synthesis and Expression of a Gene From Kringle-2 Domain of Tissue Plasminogen Activator in E. coli

The DNA fragment corresponding to the tissue plasminogen activator (tPA) sequence 174-262 (Kringle-2 domain) has been synthesized by using the solid phase phosphotriester method. The Kringle-2 domain of human tPA was expressed in Escherichia colt by secretion into the periplasmic space using the Lpp-Lac promoter and PIN-Ⅲ OmpA2 signal sequence. About two thirds of the expression product was secreted into the periplasmic space , and purified with ammonium sulfate fractionation, affinity chro-matography on Lysine-Sepharose, and FPLC-Mono Q exchange chromatography. The amino acid composition observed from the Kringle-2 purified from E. coli is identical with that expected for the 174-262 fragment of human tPA. Radio binding assay shows that the recombinant Kringle-2 domain possesses the activity of fibrin binding.     

粉末化学镀法制备的NiB／TiO2非晶态合金催化剂对环丁烯砜加氢反应的催化性能

 采用诱导沉积法及粉末化学镀法分别制备了纯态NiB及负载型NiB／TiO2非晶态合金催化剂．用XRD，ICP，SEM，TEM和DSC等手段对催化剂的物性及TiO2载体与NiB非晶态合金之间的相互作用进行了表征，考察了非晶态合金的结构、组成、形貌和热稳定性，并将其用于环丁烯砜加氢反应中．结果表明，相对于NiB而言，NiB／TiO2催化剂具有优良的热稳定性和催化活性，这缘于NiB和TiO2载体之间的相互作用及载体的分散作用．     

Studies on Artificial Imitation of Glutathione Peroxidase by Chemical Mutation

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亚磺化脱卤试剂在有机合成中的应用

本文综述了亚磺化脱卤试剂在有机合成中的应用。特别是氟烷基化反应中的应用。