Determination of diamine oxidase by a kinetic method with 2,2''-azino-di(3-ethylbenzthiazoline-6-sulfonate)

Hydrogen peroxide, the product of diamine oxidase-catalyzed putrescine or cadaverine oxidation, formed in proportion to the enzyme activity, is measured spectrophotometrically by using the above sulfonate (ABTS) and peroxidase. Only one reagent solution containing 3 mmol of putrescine or 10 mmol of cadaverine, 4 mmol of ABTS and 3000 U of peroxidase per litre of 0.2 mol l^-1 Tris—0.1 mol l^-1 HCl buffer pH 7.5 is needed. Absorbance changes are measured at 410 nm over the first 3 min of the reaction. This initial oxidation rate of the chromogen enables diamine oxidase activity up to 230 U l^-1 to be determined.     

Argentometric determination of iodide and cyanide using a depolarisation end-point

It has been shown that the depotarisation end-point technique, based on the phenomenon of a sudden out-of-balance current, produced by the establishment of a new redox potential at the indicator electrode in a pre-balanced circuit, can also be applied to precipitation titrations. The use of a platinum indicator electrode instead of one made of silver, and determination in very dilute solution, yield better results than those obtained using the classical potentiometric method.     

Thermal analysis and kinetics of the copper-lead matte roasting process

In this paper, the results of copper-lead matte investigations are presented. Investigated copper-lead matte is intermediate product of the lead production in TREPCA-Zvečan. In the first part of the paper characterization of starting material is presented, consisting of: chemical composition analysis (XRQ), scanning electron microscopy (SEM) and diffractometry (XRD). Thermal properties of investigated matte were determined using differential thermal analysis (DTA) at characteristic temperatures. Using results of induced analysis, mechanism of matte oxidation process was determined. In the second part of the paper kinetic parameters describing oxidative roasting of copper-lead matte are presented. For activation energy calculations, non-isothermal methods defined by Kissinger and Ozawa were used [1, 2]. Kinetic equation describing oxidation process was determined using Sharps method of reduced half time of reaction [3].     

Reduction of a simple ion at permeable film-coated stationary electrodes

Recently proposed chemically prepared electrodes are coated with a thin, permeable, insulating, inert film which does not react with the depolarizer, does not allow depolarization on its surface and does not change the standard constant of the depolarization rate. It only changes the diffusion coefficient of a certain ion near the surface of the electrode. In this article, the theory of a reversible reduction of a simple ion at a film-coated stationary planar electrode is developed. If the film thickness is comparable with a diffusion layer thickness, considerable changes on the i-t curves can occur, but the position of the half-wave potential will remain constant.     

Density of states for intermediate valence and Kondo systems

We calculate the density of states for the nondegenerate Anderson model for various values ofu=U/ andn _f using the perturbation theory withu as the expansion parameter. Summing all the -independent self-energy diagrams, we use the Friedel sum rule and Ward identities to express the physical quantities in terms of the remaining -dependent part of the self-energy, which we evaluate to the 2nd order. The results for the spin and charge susceptibilities obtained in such a way compare rather well with the Bethe-ansatz results. The density of states exhibits different features in different parts of the parameter space. In Kondo region (u>1,n _f 1, i.e., – _f ~U/2), we obtain a many-body resonance (half-width T _K ) around the Fermi level and two broad peaks () at about _f +n _f U and _f +U. In the VF region (u>1, and | _f |) we obtain only two peaks (), one at about _f and one between _f +n _f U and _f +U. The consequences regarding the shape of the photoemission and inverse photoemission spectra of Ce intermetallics are discussed.     

The elliptical gaussian wave transformation due to diffraction by an elliptical hologram

Realized as an interferogram of a spherical and a cylindrical wave, the elliptical hologram is treated as a plane diffracting grating which produces Fresnel diffraction of a simple astigmatic gaussian incident wave. It is shown that if the principal axes of the incident beam coincide with the principal axes of the hologram, the diffracted wave field is composed of three different astigmatic gaussian waves, with their waists situated in parallel but distinct planes. The diffraction pattern, observed on a transverse screen, is the result of the interference of the three diffracted wave components. It consists of three systems of overlapped second-order curves, whose shape depends on the distance of the observation screen from the hologram, as well as on the parameters of the incident wave beam and the hologram. The results are specialized for gratings in the form of circular and linear holograms and for the case of a stigmatic gaussian incident wave, as well as for the normal plane-wave incidence on the three mentioned types of hologram.     

Spektroskopische Untersuchungen der Metallkomplexe des 2,5-Dibenzoyl-3,4-dihydroxyselenophens

Zusammenfassung Eine Reihe von Metallkomplexen des 2,5-Dibenzoyl-3,4-dihydroxy-selenophens wurden spektroskopisch untersucht. Das Verhalten des Wismutkomplexes und die Möglichkeiten einer spektrophotometrischen Wismutbestimmung auf diesem Wege wurden eingehend studiert.

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Summary A spectroscopic examination was made of a series of metal complexes of 2,5-dibenzoyl-3,4-dihydroxyselenophen. The behavior of the bismuth complex and the possibilities of a spectrophotometric method of determining bismuth in this manner were studied thoroughly.

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Herrn Univ.-Prof. Dr.Hans Lieb zum 80. Geburtstag gewidmet.     

Contamination level of Danube river sediments

The paper presents a survey of the contamination level of the Danube river sediments along its course, between 849 km and 1162 km, during the period 1985–1988. Sampling was performed on several locations in the seasons with extreme hydrological conditions (spring, autumn).     

Surface recombination in breakdown time delay experiments: Effect of different cathode materials

The late afterglow in nitrogen with iron electrode is studied by the breakdown time delay method, i.e., by measuring the breakdown time delay t_d as a function of the afterglow time . It is proposed that the cause of the secondary electrons initiating the breakdown is the energy of the surface recombination of nitrogen atoms on the iron electrode. The gas-phase and macrokinetic diffusive models are used to describe the experimental breakdown time delay data. By fitting the theoretical curve to the experimental data: (1) it has been confirmed that the recombination on the molybdenum glass is of the second order and the value of the surface recombination coefficient is determined at 4 mbar; (2) it has been shown that the surface recombination on the iron electrode is of the second order, and the effective recombination coefficients are determined; (3) the analytical form of the recombination coefficient as a function of the adsorption characteristics of surfaces and the pressure of the parent gas has been derived. In addition, the orders of surface recombination on the molybdenum-, aluminum-, and gold-plated electrode were determined by the same method.