Molecular umbrella-assisted transport of an oligonucleotide across cholesterol-rich phospholipid bilayers

A series of molecular umbrella conjugates, derived from cholic acid, deoxycholic acid, spermidine, lysine, and 5-mercapto-2-nitrobenzoic acid, have been synthesized and found capable of transporting an attached 16-mer oligonucleotide (S-dT16) across liposomal membranes made from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyldglycerol (POPG), and cholesterol [POPC/POPG/cholesterol (65/5/30; mol/mol/mol, v/v/v)] at 37 degrees C. Those molecular umbrellas containing four choloyl (or deoxycholoyl) groups resulted in significantly faster rates of transport as compared to those containing only two such moieties. A model that accounts for these membrane transport processes is proposed.     

Phase-Averaged Vortex Train Flow Generated by Plasma DBD Actuator

It will be shown how plasma actuator can generate wall-jet-like flow or train of periodical vortices depending on the generator setting. For generation the high-frequency high-voltage AC is used. Low-frequency modulation of the supply voltage is required to generate vortices. Data acquisition will be performed using time-resolved PIV technique. Phase-averaging will be studied from two different perspectives. Firstly, sampling of phases will be ensured using trigger that is contained in the PIV software and, secondly, phase-averaged flow will be computed from two main modes of POD analysis. The generated flow patterns are to be applied for control of a boundary layer. (© 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)     

Dynamics of flow near a separation point

Dynamical behavior of a boundary layer separation is studied using the experimental approach. The dynamical nature of the phenomenon is demonstrated on a simple case of a flat–plate boundary layer in adverse pressure gradient. The Time–Resolved PIV technique was utilized for monitoring instantaneous structure of the separation region and its time development. Distinctive coherent structures and their dynamical behavior were identified using the Proper Orthogonal Decomposition method. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)