MALDI imaging mass spectrometry of lipids by adding lithium salts to the matrix solution

Mass spectrometry imaging of lipids using MALDI–TOF/TOF mass spectrometers is of growing interest for chemical mapping of organic compounds at the surface of tissue sections. Many efforts have been devoted to the best matrix choice and deposition technique. Nevertheless, the identification of lipid species desorbed from tissue sections remains problematic. It is now well-known that protonated, sodium- and potassium-cationized lipids are detected from biological samples, thus complicating the data analysis. A new sample preparation method is proposed, involving the use of lithium salts in the matrix solution in order to simplify the mass spectra with only lithium-cationized molecules instead of a mixture of various cationized species. Five different lithium salts were tested. Among them, lithium trifluoroacetate and lithium iodide merged the different lipid adducts into one single lithium-cationized species. An optimized sample preparation protocol demonstrated that the lithium trifluoroacetate salt slightly increased desorption of phosphatidylcholines. Mass spectrometry images acquired on rat brain tissue sections by adding lithium trifluoroacetate showed the best results in terms of image contrast. Moreover, more structurally relevant fragments were generated by tandem mass spectrometry when analyzing lithium-cationized species.     

Towards new molecular photocatalysts for CO2 reduction: photo-induced electron transfer versus CO dissociation within [Os(NN)(CO)2Cl2] Complexes

Optical excitation in the visible region of trans-(Cl)-[Os(bpy)(CO)(2)Cl(2)] (bpy=2,2'-bipyridine; C1) and trans-(Cl)-[Os(dmbpy)(CO)(2)Cl(2)] (dmbpy=4,4'-dimethyl 2,2'-bipyridine; C2) is known to induce the common CO dissociation reaction. However, the quantum yield of the reactions is less than 0.15, although C1 and C2 display pronounced photoluminescence in the visible region at room temperature with a lifetime of few tens of nanoseconds. Taking into account the characteristics of their emitting state, we have investigated the capability of C1 and C2 to act as a photosensitiser in redox reactions in different solvents (MeCN, PrCN and DMF). The efficient oxidation and reduction of both complexes under continuous irradiation in the presence of a sacrificial electron acceptor or donor is reported here. The photo-induced transformations and the nature of the resulting compounds were analysed by UV/Vis and IR spectroscopies and cyclic voltammetry. Photo-induced oxidation of C1 and C2 leads to the corresponding monocarbonyl oxidised species, whereas photo-induced reduction under argon leads mainly to the formation of the corresponding Os-bonded molecular wires P1 and P2 after exchange of two electrons associated with the loss of two chloro ligands. The chemical yield of the latter reaction (around 65%) becomes quantitative by adding [Ru(bpy)(3)](2+) as an external redox photosensitiser. This behaviour has been used to photocatalyse the two electron, two proton conversion of CO(2) to CO. Turnover numbers (TON) of 11.5 and 19.5 have been obtained respectively for C1 and C2 after 4.5 h of irradiation under CO(2) in DMF with triethanolamine as the electron donor. TON can be slightly increased by adding [Ru(bpy)(3)](2+) to the solution.     

Recherches théoriques sur la structure électronique de molécules contenant des atomes hybridés en sp

Résumé La structure de la molécule d'allène est étudiée à l'aide de la méthode LCAO améliorée. On met en évidence la nécessité d'introduire d'une part l'interaction des systèmes et avec les liaisons C-H et d'autre part un déplacement d'électrons dans les liaisons C-H. La distance interatomique C₁-C₂ est alors bien retrouvée et les charges nettes, presque nulles, sont en accord avec ce que suggère la réactivité chimique de la molécule. La transition permise A ₁ B ₂ est trouvée en bonne place; les trois autres étant un peu basses. Le problème de la réduction des intégrales faisant intervenir la couche 1s est abordé.

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The improved LCAO theory is used to study the structure of the allene molecule. We show that we must introduce on the one hand the interaction of the and systems with the C-H bonds and on the other hand an electrons displacement in the C-H bonds. The bond distance C₁-C₂ is then found in good agreement with the experimental value and the net charges, almost null, are in agreement with what suggests the chemical reactivity of the molecule. The allowed transition A ₁ B ₂ is found at the right place; the other three being a little low. The problem of the integrals reduction, making to intervene the 1s shell, is approached.

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Zusammenfassung Die Struktur von Allen wird mit Hilfe der verbesserten LCAO-Methode untersucht. Es wird gezeigt, daß eine Wechselwirkung zwischen - und -System mit den C-H-Bindungen und eine Elektronenverschiebung längs der C-H-Bindungen eingeführt werden müssen. Der C₁-C₂-Bindungsabstand stimmt mit der Erfahrung überein. Die Nettoladungen sind fast Null, wie die chemische Reaktivität zeigt. Der erlaubte Übergang A ₁ B ₂ wird gut plaziert angegeben; aber die drei anderen liegen ein wenig zu tief. Das Problem der Integralreduktion mit 1s-Atomorbitalen wird behandelt.

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Nous tenons à remercier ici M. le Professeur André Julg (Laboratoire de Chimie Théorique, Faculté des Sciences, Marseille) pour les nombreuses discussions que nous avons eues avec lui sur ce sujet, et qui a bien voulu relire le manuscrit.

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Les calculs numériques ont été effectués par nos soins sur l'ordinateur PB 250 du Centre de Calcul Numérique de la Faculté des Sciences de Marseille.     

Through-space intramolecular palladium rearrangement in substituted aryl complexes: theoretical study of the aryl to alkylpalladium migration process

DFT/B3LYP calculations have been carried out to study intramolecular 1,n palladium shifts (n = 3-5) between sp2 and sp3 carbon atoms in alkylarylpalladium systems. Such shifts, which also involve a concomitant exchange with a hydrogen atom of the alkylaryl ligand, are quite often a pivotal step of several organic transformations mediated by palladium complexes. We show that the intimate mechanism for the 1,3 shift corresponds to a Pd(IV) pathway, whereas a Pd(II) pathway is favored in the case of 1,5 migrations. In the case of 1,4 migrations, both mechanisms are competitive. The Pd(IV) pathway can involve either a true Pd(IV) intermediate (oxidative addition/reductive elimination mechanism) or a Pd(IV) transition state (oxidative hydrogen migration mechanism). The energy barrier is very high for the 1,3 palladium shift, making this process very unlikely, in contrast to the other ones which have enthalpy barriers ranging between 22.8 kcal mol-1 (for the 1,5 shift) and 31.9 kcal mol-1 (for the least favorable 1,4 shift studied here). All of these results are in line with our previous results for palladium shifts between two sp2 carbon atoms. In addition, the sp2 to sp3 shifts have been found to be rather exothermic owing to the possibility for the alkylaryl ligand in the product to achieve a eta3 coordination mode. This eta3 coordination mode results either from the shift itself (1,3 case) or from a subsequent rearrangement that comprises a chain-running mechanism within the alkyl chain bound to the metal (for n > 3).     

How does internal motion influence the relaxation of the water protons in Ln(III)DOTA-like complexes?

Taking advantage of the Curie contribution to the relaxation of the protons in the Tb(III) complex, and the quadrupolar relaxation of the 17O and 2H nuclei on the Eu(III) complex, the effect of the internal motion of the water molecule bound to [Ln(DOTAM)(H2O)]3+ complexes was quantified. The determination of the quadrupolar coupling constant of the bound water oxygen chi(Omicron)(1 + eta(Omicron)2/3)1/2 = 5.2 +/- 0.5 MHz allows a new analysis of the 17O and 1H NMR data of the [Gd(DOTA)(H2O)]- complex with different rotational correlation times for the Gd(III)-O(water) and Gd(III)-H(water) vectors. The ratio of the rotational correlation times for the Ln(III)-H(water) vector and the overall rotational correlation time is calculated tau(RH)/tau(RO) = 0.65 +/- 0.2. This could have negative consequences on the water proton relaxivity, which we discuss in particular for macromolecular systems. It appears that the final effect is actually attenuated and should be around 10% for such large systems undergoing local motion of the chelating groups.     

Synthesis and polymerization of racemic and optically active β-substituted β-propiolactones. II: β-monosubstituted and β-disubstituted monomers and polymers with different optical purities

β-(trichloromethyl)-β-propiolactone (CCl₃-PL), β-(trifluoromethyl,methyl)-β-propiolactone (CF₃, Me-PL) and β-(trifluoromethyl,ethyl)-β-propiolactone (CF₃,Et-PL) have been obtained by the reaction of ketene with chloral, 1,1,1-trifluoroacetone and 1,1,1-trifluorobutanone, respectively. Chiral catalysis lead to optically active monomers. The enantiomeric excess of the lactones has been measured by ¹H-NMR spectroscopy, in the presence of 2,2,2-trifluoro-1-(9-anthryl)ethanol or an europium chiral shift reagent. Polymerizations have been carried out in bulk or in toluene, at 60°C or 80°C, using mainly organometallic initiators. The Polymers become insoluble and crystalline at enantiomeric excesses over 80% for CCl₃-PL and 70% for CF₃,Me-PL. Melting temperatures were recorded from 238 to 268°C for poly(CCl₃-PL) and from 78 to 100°C for poly(CF₃,Me-PL), depending upon the molecular weight and the enantiomeric excess. The ¹³C-NMR specroscopy of poly(CCL₃-PL) indicates that the polymerization of the corresponding lactone leads to polymers of increasing degrees of isotacticity with the enantiomeric excess of the monomer.     

Application of a chemical equilibrium model to thermodynamic functions of transfer of alcohols from water to aqueous surfactants

In ternary aqueous solutions, hydrophobic solutes such as alcohols tend to aggregate with surfactants to form mixed micelles. These systems can be studied by meas of the functions of transfer of hydrophobic solutes from water to aqueous solutions of surfactant. These thermodynamic functions often go through extrema in the critical micellar concentration (CMC) region of the surfactant. A simple model based on interactions between surfactant and hydrophobic solute monomers, on the distribution of the hydrophobic solute between water and the micelles and on the shift in the CMC induced by the hydrophobic solute, can simulate the magnitude and trends of the transfer functions using parameters which are mostly derived from the binary systems. In order to check the model more quantitatively, volumes and heat capacities of transfer of alcohols from water to aqueous solutions of a nonionic surfactant, octyldimethylamine oxide, were measured. A quantitative agreement was achieved with three adjustable parameters. Good fits are also obtained for the transfers to the ionic surfactants, octylamine hydrobromide and sodium dodecylsulfate. When the equilibrium displacement contribution is small, the distribution constants and the partial molar properties of the alcohols in the micellar phase agree well with the parameters obtained with similar models.     

Influence of polarization and wavelength on two-photon excited luminescence of single gold nanospheres

The Brownian rotation of a nearly spherical gold particle capped with ligands can be observed in the correlation profile of the intensity of the two-photon excited luminescence. Here we report on a multi-parameter study of the luminescence properties, including spectral and polarization analysis of the signal at the single particle level. First, the data confirm the role of the radiative de-excitation of the surface plasmons in the luminescence process. Secondly, the results obtained at low power indicate that the capped particle in water can be approximatively described as a spherical rotor acting in the far-field as a point-like absorption and emission dipole of fixed directions. In addition, we show that the dynamics of the ligands, induced by the heat transfer from the particle to its environment, can be partly controlled by the choice of excitation wavelength.     

Simultaneous Determination of Nickel and Cobalt as Chelates with 1-(2-Pyridylazo)-2-naphthol Using an LC Switching Column Method

A simple liquid chromatographic method was developed for the separation and simultaneous determination of cobalt and nickel as chelates with 1-(2-pyridylazo)-2-naphthol (PAN). The method, using a switching column technique for the on-line purification and separation, enables to reach the sub-microgram per litre concentration level excluding off-line sample treatment with the exception of the derivatization reaction. Two small-sized columns packed with CN- and C₄-bonded stationary phases were selected and used considering their complementary behaviour with respect to chelated Co and Ni ions. The analysis was performed within 10 min using an optimised eluent (water–acetonitrile–methanol–tetrahydrofuran, 40:45:10:5, v/v/v/v) containing Tween 40 (10^?3 M) and acetate buffer (5 × 10^?3 M, pH 4.8). Detection was performed by UV-vis spectrophotometry (λ = 565 nm) permitting to reach quantification limits of 0.9 and 0.5 μg L^?1 for Co and Ni, respectively.     

Neutron scattering studies of K(3)H(SO(4))(2) and K(3)D(SO(4))(2): The particle-in-a-box model for the quantum phase transition

In the crystal of K(3)H(SO(4))(2) or K(3)D(SO(4))(2), dimers SO(4)???H???SO(4) or SO(4)???D???SO(4) are linked by strong centrosymmetric hydrogen or deuterium bonds whose O???O length is ≈2.50 A?. We address two open questions. (i) Are H or D sites split or not? (ii) Is there any structural counterpart to the phase transition observed for K(3)D(SO(4))(2) at T(c) ≈ 85.5 K, which does not exist for K(3)H(SO(4))(2)? Neutron diffraction by single-crystals at cryogenic or room temperature reveals no structural transition and no resolvable splitting of H or D sites. However, the width of the probability densities suggest unresolved splitting of the wavefunctions suggesting rigid entities H(L1∕2) -H(R1∕2) or D(L1∕2) -D(R1∕2) whose separation lengths are l(H) ≈ 0.16 A? or l(D) ≈ 0.25 A?. The vibrational eigenstates for the center of mass of H(L1∕2) -H(R1∕2) revealed by inelastic neutron scattering are amenable to a square-well and we suppose the same potential holds for D(L1∕2) -D(R1∕2). In order to explain dielectric and calorimetric measurements of mixed crystals K(3)D((1 - ρ))H(ρ)(SO(4))(2) (0 ≤ ρ ≤ 1), we replace the classical notion of order-disorder by the quantum notion of discernible (e.g., D(L1∕2) -D(R1∕2)) or indiscernible (e.g., H(L1∕2) -H(R1∕2)) components depending on the separation length of the split wavefunction. The discernible-indiscernible isostructural transition at finite temperatures is induced by a thermal pure quantum state or at 0 K by ρ.