Interaction of Stratum Corneum Components With Benzalkonium Chloride. Isothermal titration calorimetric study

The interactions of benzalkonium chloride (BC)with components of stratum corneum, a model system of intercellular lipids in human stratum corneum and homogenized rat stratum corneum were characterized in terms of thermodynamics at pH 7.5 and 37°C. BC was strongly bound to cholesterol and cholesterol sulfate with higher affinities (10⁵~10⁶ M^-1) than to any other components of the stratum corneum by hydrophobic interaction and ionic interaction, respectively. BC binding to the model system of intercellular lipids significantly decreased only in the absence of cholesterol. It is indicated that cholesterol and its derivatives play an important role in the penetration and/or accumulation of BC in stratum corneum. This revised version was published online in July 2006 with corrections to the Cover Date.     

First synthesis and characterization of isolable thioselenenic acid,triptycene-9-thioselenenic acid

Triptycene-9-thioselenenic acid was synthesized by hydrolysis of acetyl triptycene-9-thioselenenate, the structure of which was determined by spectroscopic data and X-ray diffraction analysis.     

Waiting time problems for a two-dimensional pattern

We consider waiting time problems for a two-dimensional pattern in a sequence of i.i.d. random vectors each of whose entries is 0 or 1. We deal with a two-dimensional pattern with a general shape in the two-dimensional lattice which is generated by the above sequence of random vectors. A general method for obtaining the exact distribution of the waiting time for the first occurrence of the pattern in the sequence is presented. The method is an extension of the method of conditional probability generating functions and it is very suitable for computations with computer algebra systems as well as usual numerical computations. Computational results applied to computation of exact system reliability are also given. Department of Statistical Science, School of Mathematical and Physical Science, The Graduate University for Advanced Studies This research was partially supported by the ISM Cooperative Research Program (2002-ISM-CRP-2007).     

On nonparametric tests for symmetry

Summary This paper is concerned with an extension of the problem of testing symmetry about zero of a distribution function. In order to obtain the asymptotic null distribution of test statistics for the problem, a limit theorem is proved, which indeed plays an essential role in the asymptotic theory of testing, problem for symmetry. The Institute of Statistical Mathematics     

Waiting Time Problems in a Two-State Markov Chain

Let F ₀ be the event that l ₀ 0-runs of length k ₀ occur and F ₁ be the event that l ₁ 1-runs of length k ₁ occur in a two-state Markov chain. In this paper using a combinatorial method and the Markov chain imbedding method, we obtained explicit formulas of the probability generating functions of the sooner and later waiting time between F ₀ and F ₁ by the non-overlapping, overlapping and "greater than or equal" enumeration scheme. These formulas are convenient for evaluating the distributions of the sooner and later waiting time problems.     

Synthesis,antimicrobial activity and QSAR studies of 2,5-disubstituted benzoxazoles

In this study, a new series of 2,5-disubstituted benzoxazoles was synthesized and their structures were elucidated by elemental analysis, MASS, ¹H-NMR, ¹³C-NMR and IR spectral data. Newly and previously synthesized 2,5-disubstituted benzoxazole derivatives were evaluated for antibacterial and antifungal activity against standard strains and their drug-resistant isolates. Microbiological results showed that the compounds presented a large spectrum of activity having MIC values of 250–7.8 µg mL^?1 against the tested microorganisms. Among the newly synthesized derivatives 3–22, compound 11 was the most active against Candida krusei out of all; however, it was one dilution less potent than standard drug fluconazole. In addition, all the new and previous compounds were more active than standard drugs ampicillin trihydrate and rifampicin against Pseudomonas aeruginosa and its gentamicin-resistant isolate. The 2D-QSAR (Quantitative Structure–Activity Relationship) analysis of a set of newly and previously synthesized benzoxazoles tested for growth inhibitory activity against methicillin-resistant Staphylococcus aureus (MRSA) was also performed by using multivariable regression analysis. The activity contributions for substituent effects of these compounds were determined from the correlation equation for predictions of the lead optimization.     

Carbohydrate recognition of symmetrical tripodal receptor type tris(2-aminoethyl)amine derivatives

Carbohydrate recognition of some bioactive symmetrical tripodal receptor type tris(2-aminoethyl)amine (TAEA) derivatives was investigated. In calorimetric experiments, the highest binding constant (Ka) of compound C (C₃₅H₄₉N₅O₄S) with methyl α-d-mannopyranoside was Ka = 858 M^?1 with 1:1 stoichiometry. Formation of hydrogen bonds in binding between symmetrical tripodal receptor type compound C and sugars was suggested by the large negative values of ?H° (=?34 to ?511 kJ mol^?1). In a comparison of each set of α- and β-anomers of some monosaccharides (methyl α/β-d-galactopyranoside, methyl α/β-d-glucopyranoside, and methyl α/β-l-fucopyranoside), compound C showed that the binding constant of β-anomer was larger than that of the corresponding α-anomer, indicating higher β-anomer selectivity. The calculated energy-minimized structure of the complex of compound C with guest methyl α-d-mannopyranoside is also presented. The experimental results obtained from this work indicated that symmetrical tripodal receptor type TAEA derivative C has a lectin-like carbohydrate recognition property.     

平行于异材界面的三维椭圆平片裂纹分析

讨论了拉伸载荷作用下平行于两相材料界面的椭圆平片裂纹问题．首先,使用有限部积分概念和两相材料界面完全接合时的点力基本解导出了一组以裂纹表面位移差为未知函数的超奇异积分方程组．该组方程表明,此时三种裂纹模型同时存在;其次,在数值求解该组方程的过程中,未知函数裂纹表面位移差被近似为位移差的基本密度函数与多项式之积．基本密度函数反映了裂纹前沿应力奇性性态;最后,以拉伸载荷为例,讨论了椭圆平片裂纹与界面的距离、裂纹形状比和不同材料组合对应力强度因子的影响,并以图表形式给出。