"Mass defect" tags for biomolecular mass spectrometry

We present a new class of "mass defect" tags with utility in biomolecular mass spectrometry. These tags, incorporating element(s) with atomic numbers between 17 (Cl) and 77 (Ir), have a substantially different nuclear binding energy (mass defect) from the elements common to biomolecules. This mass defect yields a readily resolvable mass difference between tagged and untagged species in high-resolution mass spectrometers. We present the use of a subset of these tags in a new protein sequencing application. This sequencing technique has advantages over existing mass spectral protein identification methodologies: intact proteins are quickly sequenced and unambiguously identified using only an inexpensive, robust mass spectrometer. We discuss the potential broader utility of these tags for the sequencing of other biomolecules, differential display applications and combinatorial methods.     

Conditionally specified models and dimension reduction in the exponential families

We consider informative dimension reduction for regression problems with random predictors. Based on the conditional specification of the model, we develop a methodology for replacing the predictors with a smaller number of functions of the predictors. We apply the method to the case where the inverse conditional model is in the linear exponential family. For such an inverse model and the usual Normal forward regression model it is shown that, for any number of predictors, the sufficient summary has dimension two or less. In addition, we develop a test of dimensionality. The relationship of our method with the existing dimension reduction theory based on the marginal distribution of the predictors is discussed.     

Associated primes and cofiniteness of local cohomology modules

Let be an ideal of Noetherian ring R and let s be a non-negative integer. Let M be an R-module such that is finite R-module. If s is the first integer such that the local cohomology module is non -cofinite, then we show that is finite. In particular, the set of associated primes of is finite. Let be a local Noetherian ring and let M be a finite R-module. We study the last integer n such that the local cohomology module is not -cofinite and show that n just depends on the support of M.The research of the first author was supported in part by a grant from IPM (No. 83130114).The second author was supported by a grant from University of Tehran (No. 6103023/1/01).     

Independent fuzzy events

An algorithm for the reconstruction of a symmetric matrix from the spectral data is given. All cases in which the number of solutions is finite are determined. These results are applied to a certain inverse eigenvalue problem which arises in molecular spectroscopy.     

MALDI‐TOF mass spectrometry,an efficient technique for in situ detection and characterization of actinomycins

An extensive study of actinomycins was performed using matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐TOF MS). Actinomycins represent a well‐known family of peptidolactone chromopeptides with potent cytostatic and antibiotic properties. Using five well‐characterized streptomycete strains, we introduced MALDI‐TOF MS as an efficient technique for rapid in situ detection of actinomycins in surface extracts of cells picked from agar plates. By this procedure, actinomycin complexes can be investigated with high sensitivity and accuracy in a minimum of time. These studies were complemented by mass spectrometric investigation of actinomycins obtained from culture filtrate extracts and purified by high‐performance liquid chromatography to detect yet unknown actinomycin species. By feeding experiments, C‐demethyl‐actinomycins from Streptomyces chrysomallus and Streptomyces parvulus as well as hemi‐actinomycins from Streptomyces antibioticus lacking one of the two pentapeptide lactone rings were isolated and characterized as novel variants for structure–activity relationship studies. Structural characterization of the investigated actinomycins was performed by post source decay MALDI‐TOF MS. The specific features of the fragmentation patterns of the protonated and cationized forms of selected actinomycins were investigated in detail. Copyright © 2014 John Wiley & Sons, Ltd.     

Simplicial join via tensor product

Let be a field, and M and N two finitely generated graded modules over standard graded -algebras A and B, respectively. We will study generalized, sequentially, almost, and approximately Cohen–Macaulay as well as clean, and pretty clean properties of the -module through the corresponding properties of M and N. The behavior of these properties with respect to the simplicial join of two simplicial (multi)complexes will be revealed as corollaries.     

Discrete adaptive fuzzy control for asymptotic tracking of robotic manipulators

This paper presents a novel discrete adaptive fuzzy controller for electrically driven robot manipulators. It addresses how to overcome the nonlinearity, uncertainties, discretizing error and approximation error of the fuzzy system for asymptotic tracking control of robotic manipulators. The proposed controller is model-free in the form of discrete Mamdani fuzzy controller. The parameters of fuzzy controller are adaptively tuned using an adaptive mechanism derived by stability analysis. A robust control term is used to compensate the approximation error of the fuzzy system for asymptotic tracking of a desired trajectory. The controller is robust against all uncertainties associated with the robot manipulator and actuators. It is easy to implement since it requires only the joint position feedback. Compared with fuzzy controllers which employ all states to guarantee stability, the proposed controller is very simpler. Stability analysis and simulation results show its efficiency in the tracking control.     

Design and construction of hot limiter biasing system for the tokamak

A hot limiter biasing system with a simplified fast switch circuit was designed, constructed, and installed on the IR-T1 tokamak, and then the negative voltage applied to a hot limiter inserted inside the tokamak fixed limiter and the plasma current, poloidal, and radial components of the magnetic fields, loop voltage, diamagnetic flux, and the ion saturation currents in the absence and presence of the biased limiter were measured. Results of measurements of biasing effects on the plasma equilibrium behavior and edge plasma rotation are compared and discussed.     

On (Strongly) Gorenstein Von Neumann Regular Rings

In this article, we introduce and study the rings over which every module is (strongly) Gorenstein flat, which we call them (strongly) Gorenstein Von Neumann regular rings.