Biospecific thermal analyzer coupled with a flowthrough immobilized enzyme column

A thermal analyzer consisting of a pair of glass thermistors was coupled with a flowthrough immobilized enzyme reactor for the determination of ATP concentration. Hexokinase was covalently bound to poly(chloromethyl-styrene-divinyl benzene) beads and packed in the reactor. The thermal analyzer was designed so as to respond to heat generation in the reactor. A maximum temperature change was exhibited within 1.5 min after the injection of a sample solution containing ATP together with glucose. Thermal decay occurred within 1 min. The thermal analyzer responded reproducibly to ATP at a constant concentration. Assays could be repeated at 3-min intervals. The effects of sample volume and flow rate on the response were examined with regard to an empirical equation. Concentration of ATP was determined with the thermal analyzer in the range of 0-10 mM. The thermal analyzer may be a simple device for the rapid determination of ATP concentration.     

Synthesis of thromboxane A2 analogs—2 : (±)-Thiathromboxane A2

The total synthesis of (±)-11a-methano- 9,11-thiathromboxane A₂(1), the sulfur analog of thromboxane A₂ is described     

TheA n /(1) face models

Presented here is the construction of solvable two-dimensional lattice models associated with the affine Lie algebraA _n ^/(1) and an arbitrary pair of Young diagrams. The models comprise two kinds of fluctuation variables; one lives on the sites and takes on dominant integral weights of a fixed level, the other lives on edges and assumes the weights of the representations ofsl(n+1, C) specified by Young diagrams. The Boltzmann weights are elliptic solutions of the Yang-Baxter equation. Some conjectures on the one point functions are put forth.     

A new method for the synthesis of α-arylalkanoic acids by the use of 1,2-rearrangement of the aryl group

A simple synthetic method of α-arylalkanoic acids was accomplished by the use of a novel 1,2-rearrangement of the aryl group and this method was applied to the syntheses of some biologically important substances.     

Interlayer water molecules in vanadium pentoxide hydrate. 8. Dynamic properties by quasi-elastic neutron scattering

The dynamics of water molecules in the layered vanadium pentoxide hydrate, V(2)O(5).nH(2)O, were studied by quasi-elastic neutron scattering (QENS) measurements. Heterogeneity of the dynamic properties was confirmed by alpha-relaxation model analysis. Translational diffusion of monolayer and double-layer water molecules is by site-to-site diffusion and is reduced relative to that of bulk water. Water molecules lose their mobility markedly and solidify with decreasing temperature. However, mobile water remains at 253 K. Rotational diffusion coefficients are unaffected by confinement and are very similar to the bulk values determined at temperatures in the range 253-298 K. The dynamic speed characterized by QENS is much faster than that expected from the data determined by deuterium NMR (DNMR) measurements at low temperatures.     

Synthesis of thromboxane A2 analogs—4 : (±)-Dithiathromboxane A2 sodium salt

The total synthesis of (±)-dithiathromboxane A₂ sodium salt 1 was accomplished in 26 steps from methyl 4,4-dimethoxyactoacetate.     

Synthesis of thromboxane A2 analogs—3 : (+)-Thiathromboxane A2

(+)-11a-methano-9,11-thiathiromboxane A₂(1) was synthesized from prostaglandin A₂ and prostaglandin E₂.     

Electrochemical typing of blood using affinity membranes

Human blood group substance of the ABO system was immobilized in a mebrane matrix. The membrane-bound blood group substance retained its binding ability against the corresponding antibody (agglutinin) in serum. The transmembrane potential changed drastically with the agglutination of the membrane-bound blood group substance. Electrochemical typing of blood was performed with sera, using a pair of membranes with immobilized A- and B-type blood group substances. The blood type was determined by measuring the transmembrane potential across these membranes before and after the agglutination reaction. A possible re-use of the membrane-bound blood group substance by treatment with galactose is discussed.     

Transient behavior of secondary emission of ZnTe under band-to-band excitation

Time characteristics of secondary emissions have been measured in ZnTe at 82K under the excitation into the fundamental absorption band by subnanosecond light pulses. The decay time of polariton luminescence has been determined to be about 100 ps. On the other hand, the time behavior of the intensity of the sharp 2LO-phonon line has been found to coincide with that of the exciting pulse. This result is consistent with the resonance Raman scattering mechanism.