Semiconductor nanowires (NWs) are gaining significant importance in various biological applications, such as biosensing and drug delivery. Efficient and controlled immobilization of biomolecules on the NW surface is crucial for many of these applications. Here, we present for the first time the use of the CuI‐catalyzed alkyne–azide cycloaddition and its strain‐promoted variant for the covalent functionalization of vertical NWs with peptides and proteins. The potential of the approach was demonstrated in two complementary applications of measuring enzyme activity and protein binding, which is of general interest for biological studies. The attachment of a peptide substrate provided NW arrays for the detection of protease activity. In addition, green fluorescent protein was immobilized in a site‐specific manner and recognized by antibody binding to demonstrate the proof‐of‐concept for the use of covalently modified NWs for diagnostic purposes using minute amounts of material. 相似文献
Summary: The feasibility of automated, high‐throughput contact‐angle measurements is demonstrated using a modified commercial contact‐angle measuring apparatus. Droplets are automatically dispensed, analyzed and aspirated. The system automatically measures more than 100 contact angles per hour using two test liquids, and calculates surface energies using the equation of state method. A library of poly(2‐oxazoline) homo‐ and block copolymers was investigated. Polymers with a 2‐nonyl‐2‐oxazoline block were found to have considerably lower surface energies.
Setup used here for characterization of the surface properties of a library of poly(2‐oxazoline) homo‐ and block copolymers. 相似文献
We present an application of the residual-based variational multiscale turbulence modeling (RBVMS) methodology to the computation of turbulent Taylor–Couette flow at high Reynolds number. We show that the RBVMS formulation globally conserves angular momentum, a feature that is felt to be important for flows dominated by rotation, and that is not shared by standard stabilized formulations of fluid flow. Weak imposition of Dirichlet boundary conditions is employed to enhance the accuracy of the RBVMS framework in the presence of thin turbulent boundary layers near solid walls. Calculation of conservative boundary forces and torques is also presented for the case of weakly enforced boundary conditions. NURBS-based isogeometric analysis is employed for the spatial discretization, and mesh refinement is performed to assess the convergence characteristics of the proposed methodology. Numerical tests show that very accurate results are obtained on relatively coarse grids. To the best of the authors’ knowledge, this paper is the first to report large eddy simulation computations of this challenging test case. 相似文献