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61.
Here we report an effective method for protein immobilization on a surface plasmon resonance (SPR) gold chip, describing the combination of cysteine- and oligomerization domain-mediated immobilization of enhanced green fluorescent protein (EGFP) as a model protein for the purpose of orientation-controlled surface density packing. In order to facilitate the oligomerization of EGFP, the dimeric and trimeric constructs derived from GCN4- leucine zipper domain were chosen for multimeric EGFP assembly. For orientation-controlled immobilization of the protein, EGFP modified with cysteine residues showing excellent orientation on a gold chip was used as a starting protein, as previously reported in our earlier study (Anal. Chem., 2007, 79, 2680-2687). Constructs of EGFP with oligomerization domains were genetically engineered, and corresponding fusion proteins were purified, applied to a gold chip, and then analyzed under SPR. The immobilized EGFP density on a gold chip increased according to the states of protein oligomerization, as dimeric and trimeric EGFPs displayed better adsorption capability than monomeric and dimeric forms, respectively. Fluorescence measurement corroborated the SPR results. Taken together, our findings indicated that the combination of cysteine- and oligomerization domain-mediated immobilization of protein could be used in SPR biosensor applications, allowing for an excellent orientation and high surface density simultaneously. 相似文献
62.
Youcef Bouizem Salah Boulaaras Bachir Djebbar 《Mathematical Methods in the Applied Sciences》2019,42(7):2465-2474
The paper deals with some existence results for an elliptic equation of Kirchhoff‐type with changing sign data and a logarithmic nonlinearity by direct variational method, Galerkin approach, and sub‐super solutions method. Our results are natural extension of Boulaaras' work in (Math Methods Appl Sci; 41(13):5203‐5210). 相似文献
63.
In this paper, we establish three iteration methods to compute solutions for a class of (weakly) singular two-point boundary value problems (xy′)′=f(x,y), where x(0,1) and <2. We obtain the sufficient conditions for existence of a unique solution on
. Finally, we given some numerical examples. 相似文献
64.
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66.
We give a polynomial time controlled version of a theorem of M. Hall: every real number can be written as the sum of two irrational numbers whose developments into a continued fraction contain only 1, 2, 3 or 4. Mathematics Subject Classification: 03D15, 03F60, 11A55, 68Q25. 相似文献
67.
A mathematical model is presented for the determination of optimal mix of asbestos fibres to satisfy a set of desired pipe properties which vary with pipe size, class, and material availability. The approach utilizes a linear/separable programming model which is aimed at minimizing the total cost of fibres used. The fibre properties, such as reinforcing strength, processability, and length as well as cost and stocks available are taken into account in the model. Also represented are the profile of the ideal mix relative to the required composite strength, processability, and ‘dust content’. Asbestos mixes designed using the model have been implemented in the production of asbestos/cement pipes in one of the asbestos/cement pipe manufacturing plants in the Gulf area. Optimum model mixes have demonstrated significant technical and economic merits over the conventionally designed mixes. 相似文献
68.
An indirect method is outlined for the spectrophotometric determination of small amounts of perchlorate. Perchlorate is quantitatively precipitated as nitron perchlorate in the presence of excess nitron. After separating off the precipitate, the excess reagent is determined in the supernatant liquid as nitron cobaltothiocyanate. The latter compound is extracted in 30% cyclohexanone in carbon tetrachloride and the absorbance of the extract measured at 625 nm. The method is simple, reproducible, and accurate to ±1%. The interferences of other anions were also investigated. 相似文献
69.
The action of dimethylformamide in phosphorus oxychloride on acylanilides gives 2-chloro-3-substituted quinolines, which may be dechlorinated to give 3-substituted quinolines in good yield. Similarly, N-nitrosodimethylamine in phosphorus oxychloride converts acylanilides into 2-chloro-3-substituted quinoxazolines. 相似文献
70.
R. Ben Hassen A. Ben Salah A. Kallel A. Daoud J. Jaud 《Journal of chemical crystallography》2002,32(11):427-430
CH3NH3CdBr3 crystallizes in the space group Ccm21, a = 13.693(1) Å, b = 7.906(1) Å, c = 6.886(1) Å, V = 745.46(15) Å3, Z = 4, D
x
= 3.42 Mg m–3. The structure of the title compound is one-dimensional consisting of infinite chains made of face sharing CdBr6 octahedra. The methylammonium groups are situated in the space between chains. The cations and anions are joined by hydrogen bonding. 相似文献