Systematic computer-aided interpretation of infrared and raman vibrational spectra based on the crise program

The CRISE computer program is used to correlate wavenumber regions and 6 structural elements containing carbon, hydrogen and oxygen on the basis of 2 standard files with 549 infrared and Raman spectra. The degree of correlation, including score percentages and interfering percentages, is established for different types of intervals in relation to various intensity thresholds. Specific regions (score 100%, interference 0%) proved to be rare, whereas pseudo-specific regions (score < 100%, interference 0%) are normally present. The usefulness of selective regions (score 100%, interference > 0%) is doubtful. The infrared and Raman results for a structural element can differ appreciably, yet neither technique is clearly superior for interpretative purposes.     

The application of chromatography to the analysis of pharmaceutical creams

Summary The application of GLC, HPLC and TLC to the analysis of pharmaceutical creams is discussed with special attention to sample clean-up. The results of the determination of hydrocortisone acetate, salicylic acid, benzoic acid, diethyl stilbestrol, chloramphenicol, diphenhydramine HCl, tretinoin and some cream base components by reversed phase HPLC are given.Presented at the 14th International Symposium on Chromatography London, September, 1982     

Dynamic imaging of single DNA-protein interactions using atomic force microscopy

Atomic force microscopy (AFM) imaging of static DNA-protein complexes, in air and in liquid, can be used to directly obtain quantitative and qualitative information on the structure of different complexes. For example, DNA length, the location of preferential binding sites for proteins and bending of DNA as a result of the complexation can all be measured. Recording consecutive AFM images of DNA and protein molecules under conditions that they are still able to move and interact, or dynamic AFM imaging, however, can reveal information on the dynamic aspects of the interactions between these molecules. Here, an overview is given of the technical challenges that need to be considered for successful dynamic AFM imaging studies of individual DNA-protein interactions. Necessary technical improvements to the AFM set-up and the development of new sample preparation methods are described in this paper.     

Glutathione Synthesis Is Not Involved in Protection by N-Acetyl cysteine Against UVB-lnduced Systemic Immunosuppression in Mice

The photophysics of 5,10,15,20-tetraarylethynylporphyrinatozinc(II) complexes, 1 and 2, are reported. Compared to 5,10,15,20-tetraphenylporphyrinatozinc(II) (ZnTPP), the UV/visible spectra of 1 and 2 have red-shifted B and Q bands, with the Q bands of increased intensity relative to the B band. FIuorescence quantum yields and lifetimes and triplet quantum yields and lifetimes are similar to ZnTPP. However, quantum yields for in vitro singlet-oxygen generation are much larger than for ZnTPP and for 2 the quantum yield is near unity. These findings suggest that the title compounds could be potential lead compounds as sensitizers for photodynamic therapy.     

Wave operators and similarity of certain non-normal one-parameter groups

This paper starts from a self-adjoint Schrödinger operator H(0) for three particles. If the interaction is dilation-analytic, H(0) has an analytic continuation H() (>0). G(t,) (–(±,a,) defined as strong limits, when t±, of t-dependent operators. The wave operators establish transformations under which the subgroups are similar to unitary groups. The scattering matrix determined by G(t,) is diagonal with respect to a.This work was supported in part by the National Science Foundation under grant DMS-8301096.