The Study of Anti-tumor Activity of Trichosanthin by Cyclic Voltammogram

IthasbeenreportedthatTrichosanthi11(TCS),isolatedfromrkiI-ilolt)ii,hasanti-tumorI-4andanti-vira1activities5-'.Someanti-tun1orremedies,whicharedesignedfromTCS,arebeingtestedanddeveloped'-.'.StudyingthebioIogicalphenomenonbyelectrochemicalmethodsisanewhotresearchspotemerginginrecentyears".Manyresultsshowedthatnotonlytheenzymesystemsuchassuccinatedehydrogenasedisplayedthediode-likebehaviorduringtheelectrontransferprocess",butalsothehigh1yorganizedorganeIle,forexample,mitochondria,andevenintac…     

苏丹Ⅰ与牛血清白蛋白的相互作用特征

利用荧光技术研究了在人体生理酸度条件下,苏丹Ⅰ与牛血清白蛋白相互作用,发现苏丹Ⅰ对牛血清白蛋白有较强的荧光猝灭作用,用Stern-Volmer和Line Weaver-Burk方程处理荧光猝灭数据,得到了反应的结合常数、结合热力学性质和结合位点等参数。根据热力学常数确定了该药物与血清白蛋白之间的作用力类型,在此基础上依据F rster非辐射能量转移机理探讨了苏丹Ⅰ与BSA相互结合时其供体-受体间的距离。     

LaPO4纳米微粒的制备及表征

Surface-modified LaPO4 nanoparticales were synthesized in the mixture solvent of water-ethanol with the surface modification age of dialkylphosphate. The conditions of synthesis and characterization were discussed. The results indicated that nanoparticle core of LaPO4 and surface-modified layer were formed. The surface-modified nanoparticles of LaPO4 can be dispersed in organic solvent and has better lubricating properties.     

液相色谱专家系统中柱系统推荐规则的建立和验证

把分子结构和分子、离子静电作用力作为影响溶质保留值和选择性的基本点,提出了液相色谱专家系统柱系统推荐的基本规则,由此可以从溶质所包含的基团或官能团的信息进行色谱柱系统推荐。为了验证这些规则的正确性,应用几类常用的或典型的样品从分子结构通道进行液相色谱柱系统推荐,获得满意的结果。     

Synthesis and Photophysical Properties of Hetero‐Bischelated Complexes of Rhodium(III) Containing Phenyl‐Pyridin‐2‐Ylmethylene‐Amine

Three new hetero‐bischelated rhodium (III) complexes of cis‐[Rh(PA)(L)Cl₂]Cl (where PA = phenylpyridin‐2‐ylmethylene‐amine; L = 2,2′‐bipyridine, 2,2′‐dipyridylamine and 1,10‐phenanthroline) have been successfully prepared and characterized. Each complex shows high intensity bands in the UV region, and these are assigned to spin‐allowed π‐π* transitions. The medium‐intensity absorption band profile in the lower energy region can be explained by convolution of spin‐allowed CT and d‐d* transitions. The emission spectra at low temperature (77 K) of these complexes in EtOH/MeOH (4:1 v/v) are virtually identical. They all exhibit a broad, symmetric, and structureless red emission with a microsecond lifetime and hence are assigned as the d‐d* phosphorescence.     

Quality evaluation of fingerprints of herbal medicine with chromatographic data

In this study, local least squares (LLS) and principal component analysis (PCA) were applied to deal with the disturbances in a data set of chromatographic fingerprints after necessary data transformations. It has been demonstrated that PCA with standard normal variate (SNV) transformation of data led to meaningful classification of 33 different Erigeron breviscapus herbal samples. The result was also corroborated by variance squares discriminant method. The quality of herbal objects was further evaluated, and the causes of this fact have been explained from a chemical point of view. At the same time, it implied an idea for qualitative evaluation of the herbal objects with a common class pattern of chromatographic fingerprints.     

Selenium Nanoparticles Prepared from Reverse Microemulsion Process

Selenium nanoparticles were prepared by a reverse microemulsion system. Sodium selenosulfate was used as selenium source. The results showed that hydrochloric acid concentration and reaction temperature had great influence on the morphology of products. The crystalline selenium nanowires and amorphous selenium nanorods were obtained in given condition.     

盐酸吡哆辛及其制剂的二阶导数差示脉冲极谱法定量研究

建立盐酸吡哆辛及其制剂的二阶导数差示脉冲极谱定量分析方法。盐酸吡哆辛在0.1mol/L氢氧化钠溶液-0.1moL/L磷酸二氢钾溶液-水(体积比为1.2:8.8:990)的混合溶液中,于-0.140V(vs·Ag/AgCl)处出现一良好的二阶导数差示脉冲极谱峰,盐酸吡哆辛浓度在3.6×10~(-4)～9.6×10~(-4)mol/L范围内与其峰幅值呈显著的线性关系(P<0.01),线性相关系数r=0.9997,检出限为9.0nmol/L。     

Measurements of FRET in a glucose-sensitive affinity system with frequency-domain lifetime spectroscopy

We report measurements of fluorescence resonance energy transfer (FRET) for glucose sensing in an established concanavalin A–dextran affinity system using frequency‐domain lifetime spectroscopy. A dextran (MW 2000000) labeled with a small fluorescent donor molecule, Alexa Fluor 568, was used to competitively bind to a sugar‐binding protein, concanavalin A, labeled with acceptor molecule, Alexa Fluor 647, in the presence of glucose. The FRET‐quenching kinetics of the donor were analyzed from frequency‐domain measurements as a function of both glucose and acceptor‐protein concentrations using a Förster‐type decay kinetics model. The results show that the frequency‐domain measurements and donor decay kinetics can quantitatively indicate changes in the competitive binding of 0.09 μM dextran to labeled concanavalin A at a solution concentration of 10.67 μM in the presence of glucose at concentrations ranging from 0 to 224 mg/dL.