Carbamoylation of B6 vitamins

The reaction of B₆ vitamins 1–3 with cyanate, in the presence of equivalent amounts of hydrochloric acid, yields different adducts according to the structure of the starting material. Regiospecific attack on the amino group or the phenolic hydroxy group was found for 2a,b and 3a,b , respectively. From the aldehydes 1a,b , the 2H-pyrido[3,4-e]-1,3-oxazin-2-ones 7a,b were obtained through an attack on both the phenolic and aldehyde group.     

Reactivity of 2-methyl-4-(1-pyrrolidinyl)-2H-1,2-benzothiazine 1,1-dioxide towards p-toluenesulphonyl azide

The 2-methyl-2H-1,2-benzothiazin-4-(3H)-one 1,1 dioxide ( 2 ), obtained according to a new, one-pot method, is transformed into the pyrrolidino enamine 3 . Reaction of p-toluenesulphonyl azide with 3 gives, via an unstable triazoline adduct which loses nitrogen, the two isomeric tosylamino derivatives 4 and 5 . The structures have been assigned by exhaustive nmr analysis and some aspects on their formation and chemical behaviour are discussed.     

A polarizable continuum model for molecules at diffuse interfaces

In this work we illustrate an extension of the polarizable continuum model to describe solvation effects on molecules at the interface between two fluid phases (liquid/liquid, liquid/vapor). This extension goes beyond the naive picture of the interface as a plane dividing two distinct dielectrics, commonly employed in continuum models. The main feature of the model is the use of a diffuse interface with an electric permittivity depending on the position. This characteristic clearly allows the study of simple interfaces as well as more complex membrane or multilayer structures. Moreover the smooth variation of the permittivity in the diffuse interface, in contrast to the sharp boundary between two regions, overcomes the numerical divergences due to charges placed at the boundary. The implementation of the model relies on the integral equation formalism, which allows one to calculate the reaction field acting on a molecule immersed in a dielectric environment once the proper Green's function is known. In the present case, such a Green's function is obtained numerically, allowing a large flexibility in the choice of the dielectric permittivity profile. The applications have been selected with the aim of illustrating the capabilities of the model; its present limitations are also discussed.     

Eptastigmine,nicotinamide and nicotinic acid determination using an inhibition enzyme sensor; application to pharmaceutical analysis

An enzyme inhibition biosensor, developed in our laboratory and previously used for the analysis of compounds with anticholinesterase activity (e.g. physostigmine, neostigmine, pyridostigmine nicotine and organophosphorus compounds) has now been tested for the analysis of another recently synthesized cholinesterase inhibitor, i.e. eptastigmine. In addition nicotinic acid and nicotinamide, although displaying weaker inhibition properties, were also tested in pharmaceutical products using the same inhibition enzyme sensor. The biosensor consisted of a hydrogen peroxide amperometric electrode coupled to a functionalised nylon membrane chemically bonding both the enzymes butyrylcholinesterase and choline oxidase; a butyrylcholine standard solution in glycine buffer acted as substrate. The response of the system to all the inhibitors considered was characterised completely and the analysis of several pharmaceutical formulations containing nicotinamide or nicotinic acid was also performed.     

Neutral organic lewis acids of π type

The interaction of some neutral acids of π type, bearing appropriate unsymmetrical substitutions at the C?C group with some selected bases (H₂O, NH₃, OH^?), is compared with that of the parent compound of a new set of neutral π acids, bearing symmetrical substitutions at the C?C group with the same bases. The analyses of the interaction energy, performed according to two decomposition schemes, with and without the counterpoise corrections, make clear the similarity of symmetrically and unsymmetrically substituted neutral organic acids.     

Metal-cinnamic acids interactions. Part III. Synthesis and study of copper(II) complexes of 3, 4- and 3, 5-dimenthoxycinnamic acid

Summary Copper(II) complexes derived from substituted cinnamic acids 3, 4-dimethoxycinnamic acid (3, 4-DMCH) and 3, 5-dimethoxycinnamic acid (3, 5-DMCH), of the formula [Cu(3, 4-DMC)₂]·H₂O (1), [Cu(3, 5-DMC)₂]·H₂O (2) were prepared. The magnetic properties of the complexes suggest dimeric structures typical of copper(II) acetate monohydrate-like complexes. X-band e.s.r. spectra of polycrystalline samples at low temperature are typical of triplet state systems S=1. Their ability to catalyze the aerial oxidation of 3, 5-di-t-butylcatechol was measured spectrophotometrically at 30°C. The complexes are models for oxidases.     

Reference-free NOE NMR analysis

Nuclear Overhauser Effect (NOE) methods in NMR are an important tool for 3D structural analysis of small molecules. Quantitative NOE methods conventionally rely on reference distances, known distances that have to be spectrally separated and are not always available. Here we present a new method for evaluation and 3D structure selection that does not require a reference distance, instead utilizing structures optimized by molecular mechanics, enabling NOE evaluation even on molecules without suitable reference groups.

A quantitative Nuclear Overhauser Effect (NOE) analysis approach that avoids the use of and internal reference distance to perform molecular configuration selection.     

Synthesis of some 2-(heteroarylamino)benzimidazoles and their cyclization with phosgene

2-(Benzimidazol-2-ylamino)pyridine (4a) , 2-(benzimidazol-2-ylamino)pyrazine (4b) , and 2-(benzimidazol-2-ylamino)thiazole (4c) underwent a ring-closure reaction on treatment with phosgene affording 6H-pyrimido-[1′,2′:5,4][1,3,5]triazino[1,2-a]benzimidazol-6-one (1a) , 6H-pyrazino[1′,2′:5,4][1,3,5]triazino[1,2-a]benzimidazol-6-one (1b) , and 5H-thiazolo[2′,3′:4,5][1,3,5]triazino[1,2-a]benzimidazol-5-one (1c) respectively. The structure of these hitherto unknown heterocyclic systems was confirmed by their ir and mass spectra.     

Reaction of tetrahydrobenz[a]acridinones with hydroxylamine hydrochloride. VII

Oximation of ortho-substituted phenylbenz[a]acridinones using hydroxylamine hydrochloride, sodium hydroxide and ethanol as the solvent gave always the benzoquinacridine N-oxide 2 . Oximation of para-substituted phenylbenz[a]acridinones, however, gave only the corresponding oximes. The structure of all products was corroborated by ir, ¹H and ¹³C-nmr and mass spectral data. Theoretical calculations support the experimental findings.     

A Layer‐by‐Layer Film of Chitosan in a Taste Sensor Application

Chitosan is alternated with sulfonated polystyrene (PSS) to build layer‐by‐layer (LBL) films that are used as sensing units in an electronic tongue. Using impedance spectroscopy as the principle method of detection, an array using chitosan/PSS LBL film and a bare gold electrode as the sensing units was capable of distinguishing the basic tastes – salty, sweet, bitter, and sour – to a concentration below the human threshold. The suitability of chitosan as a sensing material was confirmed by using this sensor to distinguish red wines according to their vintage, vineyard, and brands.

PCA Plot for red wine samples obtained from impedance measurements at 1 kHz for the sensor array comprising a 3‐bilayer chitosan/PSS film and a bare gold electrode.