TGF-β1 induction of the adenine nucleotide translocator 1 in astrocytes occurs through Smads and Sp1 transcription factors

Background  

The adenine nucleotide translocator 1 (Ant1) is an inner mitochondrial membrane protein involved with energy mobilization during oxidative phosphorylation. We recently showed that rodent Ant1 is upregulated by transforming growth factor-beta (TGF-β) in reactive astrocytes following CNS injury. In the present study, we describe the molecular mechanisms by which TGF-β1 regulates Ant1 gene expression in cultured primary rodent astrocytes.     

Complications of collagen injection of the vocal fold: report of several unusual cases and review of the literature

Injection laryngoplasty is one of the most frequently performed procedures in patients with voice complaints. Various biomaterials have been used to medialize vocal folds or to treat symptoms of vocal fold scar. The ideal biomaterial would be easily injected through a fine-gauge needle, well tolerated, and long lasting. Injectable collagen preparations fulfill at least two of these criteria, and collagen has been used widely for vocal fold injections. MATERIALS AND METHODS: We present a retrospective review of two unusual complications of collagen injection and a review of the relevant literature on the complications of medical use of collagen compounds. RESULTS: Two patients in whom collagen was injected formed firm submucosal deposits that interrupted the normal mucosal wave and produced significant dysphonia. Surgical removal of these deposits restored the mucosal wave and improved voice quality. Management of this unusual complication of human collagen injection in the vocal fold has not been reported previously. Other complications of collagen injection include hypersensitivity reactions to bovine collagen, local abscess formation at injection sites, and possibly induction of collagen vascular disease in some patients. CONCLUSIONS: Although collagen injections of the vocal fold rarely result in complications, physicians using collagen must be familiar with the types of complications that can occur. Proper diagnosis and prompt management of complications can result in good outcomes.     

Thickness dependence of free‐standing thin films

Below a critical thickness, of about 60 nm, the glass transition temperature of polystyrene (PS) films decreases with film thickness, as demonstrated using free‐standing films. A geometrical model is developed here describing this phenomenon in the case of ideal (Gaussian) chains. This model, which can be considered as an application of the free volume model, assumes that the decrease of the glass transition temperature from thick to ultrathin films is due to the modification of the interpenetration between neighboring chains. The theoretical curve deduced from the model is in excellent agreement with the PS experimental results, without using any adjustable parameters. From these results, it can be concluded that new chain motions, usually buried in bulk samples, are expressed by the presence of the surface. © 2006 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 45: 10–17, 2007     

Petrographic/geochemical studies of primary and alteration–weathering minerals in garnetiferous ultramafic xenoliths–basanite, Tartous Province, NW Syria

Kimberlitic–pyropic peridotite–xenolites, probably of Jurassic–Cretaceous age, were found mixed with a younger Upper Tertiary basanitic diabase, as flow texture of plagioclase laths and ilmenite rods around those xenoliths indicated. Mafic–ultramafic rocks were crushed, sheared, and cropped along a creek about 15–18 km NE of the town of Dreikeesh, NW Syria. ⁴⁰K–⁴⁰Ar isotopic dating of a pure fresh black cpx sample, collected from the peridotite xenoliths, yielded an age of about 70 Ma. This age is concurrent to the time when Africa, Eurasia, and America were part of the super continent Pangaea. It also suggests that kimberlite–pyropic peridotitic rocks were located within a cratonic pipe prior to their 2000-km eastward journey (starting from the Mid-Atlantic Rift). Sampled outcrops were located within a ring of about 40 km diameter, considered to be a dome consisting of one or more clusters of kimberlitic pipes. The dome structure, mostly covered with Upper Jurassic–Cretaceous carbonate beds, was tilted westward, and rifted and sheared along its eastern edge. Tilting and crushing were accomplished after the opening of the Red Sea in the Miocene, and the counterclockwise movements of the Arabian plate, which folded the carbonate beds to form the N–S-trending Alawite mountain range along the Syrian coast. Olivine, cpx, and pyrope were the major phases in peridotite. Pyrope, including its Cr–Ni-contents, was found to be the best indicator to decipher the temperature–pressure (T–P) conditions for the system. The inferred temperature was found to be about 1460 °C, at a pressure of 62 kb (around 207 km-depth). Petrographic studies revealed many zoned, resorbed, octahedral and non-metasomatized tiny grains, associated with partly to wholly metasomatized and iddingsitized olivines.     

Unsupervised constrained radar imaging of low resolution targets

A linear spectral estimation technique, the PDFT algorithm, is used as part of a nonlinear iterative reconstruction scheme to obtain improved radar images. The iterative PDFT algorithm is used to address the limited resolution problem inherent to imaging objects buried in soil and hidden under foliage. This is achieved by subsequent application of two properties of the PDFT algorithm: the energy parameter of the PDFT algorithm is used to determine the target shape, while the shape information in turn is used to obtain super-resolved images. We describe algorithms able to exploit both properties automatically and without manual intervention. Two methods are investigated in particular, one iteratively optimizing the constraints by monitoring the energy parameter, the other method computing energy values for all points, from which a weighted prior function is determined. In addition, we discuss variants of both algorithm which provide an optimized trade-off between computation time and performance. Additional attention is given to situations, where a known target is embedded in an unknown background. Imaging results are presented for both synthetic and measured data.     

Analysis of some combination-overtone infrared bands of SO3

Several new infrared absorption bands for ³²S¹⁶O₃ have been measured and analyzed. The principal bands observed were ν₁+ν₂ (at 1561 cm⁻¹), ν₁+ν₄ (at 1594 cm⁻¹), ν₃+ν₄ (at 1918 cm⁻¹), and 3ν₃ (at 4136 cm⁻¹). Except for 3ν₃, these bands are very complicated because of (a) the Coriolis coupling between ν₂ and ν₄, (b) the Fermi resonance between ν₁ and 2ν₄, (c) the Fermi resonance between ν₁ and 2ν₂, (d) ordinary l-type resonance that couples levels that differ by 2 in both the k and l quantum numbers, and (e) the vibrational l-type resonance between the A₁^′ and A₂^′ levels of ν₃+ν₄. The unraveling of the complex pattern of these bands was facilitated by a systematic approach to the understanding of the various interactions. Fortunately, previous work on the fundamentals permitted good estimates of many constants necessary to begin the assignments and the fit of the measurements. In addition, the use of hot band transitions accompanying the ν₃ band was an essential aid in fitting the ν₃+ν₄ transitions since these could be directly observed for only one of four interacting states. From the hot band analysis we find that the A₁^′ vibrational level is 3.50 cm⁻¹ above the A₂^′ level, i.e., r₃₄=1.75236(7) cm⁻¹. In the case of the 3ν₃ band, the spectral analysis is straightforward and a weak Δk=±2, Δl₃=±2 interaction between the l₃=1 and l₃=3 substates locates the latter A₁^′ and A₂^′ “ghost” states 22.55(4) cm⁻¹ higher than the infrared accessible l₃=1 E^′ state.