Elastic properties of a protein-polymer-grafted surface

Surfaces grafted with poly(methyl methacrylate) (PMMA) and streptavidin were synthesized through click chemistry to investigate the role of surface stiffness on protein adsorption as the hydrophilic and hydrophobic surface coverage of the substituents vary. Surface topographies coupled with the nanoindentation results indicated that, with the appropriate selections of polymer coverage and chain length, the extent of non-specific protein adhesion could be controlled by the hydrophobic interactions between PMMA, biotin, and streptavidin. It was shown that, when the molecular weight and stiffness of PMMA was close to that of streptavidin, patchy PMMA morphologies were obtained, which help inhibit the non-specific adsorption of streptavidin.     

Double-layered nanoparticle stacks for spectro-electrochemical applications

Here we present a surface based on double-layered nanoparticle stacks suitable for spectro-electrochemical applications. The structure is formed on a continuous gold layer by a two-dimensional periodic array of stacks of gold and tantalum pentoxide nanodisks. Reflection spectra in the visible wavelength region showed the multiple-resonant nature of surface plasmon (SP) excitations in the nanostructure, which is in good agreement with simulations based on a finite-difference-time-domain method. The multiple SP resonances can be tuned to various wavelength regions, which are required for simultaneous enhancement at excitation and emission wavelengths. Cyclic voltammetry measurements on the nanostructure proved the applicability of electrochemical methods involving interfacial redox processes.     

C3-triiodocyclotriveratrylene as a key intermediate to fluorescent probes: application to selective choline recognition

A new strategy to obtain fluorescent cyclotriveratrylene (CTV) probes is proposed. The key intermediate, a triiodo CTV, is prepared in 3 steps with 47% overall yield. The whole synthesis requires only one purification step. The potential of this triiodo CTV as an intermediate is illustrated through the synthesis of a fluorescent phosphorylated probe that is able to bind choline and acetylcholine in pseudo-physiological conditions, with selectivity towards choline. As a consequence, this intermediate should allow us to rapidly form a library of probes in order to highlight the most promising ones.     

Bandwidth of bipartite permutation graphs in polynomial time

We give the first polynomial-time algorithm that computes the bandwidth of bipartite permutation graphs. Bandwidth is an NP-complete graph layout problem that is notorious for its difficulty even on small graph classes. For example, it remains NP-complete on caterpillars of hair length at most 3, a very restricted subclass of trees. Much attention has been given to designing approximation algorithms for computing the bandwidth, as it is NP-hard to approximate the bandwidth of general graphs with a constant factor guarantee. The problem is considered important even for approximation on restricted classes, with several distinguished results in this direction. Prior to our work, polynomial-time algorithms for exact computation of bandwidth were known only for caterpillars of hair length at most 2, chain graphs, cographs, and most interestingly, interval graphs.     

Statistical Assessment of Counter-Flow Vortex Tube Performance for Different Nozzle Numbers,Cold Mass Fractions,and Inlet Pressures Via Taguchi Method

Abstract

In this article, the effect and optimization of process parameters in a counter-flow vortex tube on temperature difference were investigated through the Taguchi method. The experiments were planned as per Taguchi's L27 orthogonal array with each experiment performed under different conditions of inlet pressure, nozzle number, and cold mass fraction. By means of analysis of variance and regression analysis, the effects of factors and their interactions on temperature difference were determined and modeled with a correlation coefficient of 93.5%. Accordingly, it was observed that temperature difference goes up with the increase in inlet pressure, and the cold mass fraction and decreases with the increase in nozzle number. In addition, the optimum settings of process parameters maximizing the temperature difference are an inlet pressure of 650 kPa, a nozzle number of 2, and a cold mass fraction of 0.7. Finally, confirmation tests verified that the Taguchi method was successful in the assessment of vortex tube parameters for temperature difference.     

Polystyrene-supported diarylprolinol ethers as highly efficient organocatalysts for Michael-type reactions

α,α-Diphenylprolinol methyl- and trimethylsilyl ethers anchored onto a polystyrene resin have been prepared by a copper-catalyzed azide-alkyne cycloadditions (CuAAC). The catalytic activity and enantioselectivity displayed by the O-trimethylsilyl derivative are comparable to those exhibited by the best known homogeneous catalysts for the addition of aldehydes to nitroolefins and of malonates or nitromethane to α,β-unsaturated aldehydes. The combination of the catalytic unit, the triazole linker, and the polymeric matrix provides unprecedented substrate selectivity, in favor of linear, short-chain aldehydes, when the organocatalyzed reaction proceeds by an enamine mechanism. High versatility is noted in reactions that proceed via an iminium ion intermediate. The catalytic behavior of polystyrene-supported α,α-diphenylprolinol methyl ether was also evaluated in asymmetric Michael addition reactions. As a general trend, the CuAAC immobilization of diarylprolinol ethers onto insoluble polystyrene resins offers important operational advantages, such as high catalytic activity, easy recovery from the reaction mixture by simple filtration, and the possibility of extended reuse.