A reliable and validated LC–MS method was established for a hexokinase inhibitor study based on adenosine 5′-triphosphate (ATP) determination. By adding 5 mM ammonium acetate in the aqueous phase, this method enabled the determination of ATP by LC–MS and greatly increased the MS signal of ATP. This method was used in the study of the anticancer mechanism of Momordica cochinchinensis, an exact ingredient which had exhibited certain hexokinase inhibitor activity. This might reveal the anticancer mechanism of Momordica cochinchinensis. 相似文献
Multifunctional fibers have attracted widespread attention due to applications in flexible smart wearable devices. However, simultaneously obtaining a strong and functional woven fiber is still a great challenge owing to the conflict between the properties mentioned above. Herein, mechanically strong and highly conductive cellulose/carbon nanotube (CNT) composite fibers were spun using an aqueous alkaline/urea solution. The microstructure as well as physical properties of the resulting fibers were characterized via scanning electron microscopy, infrared spectroscopy, mechanical and electrical measurement. We demonstrated that carboxylic CNTs can be well dispersed in alkali/urea aqueous systems which also dissolved cellulose well. The subsequent wet spinning process aligned the CNTs and cellulose molecules inside the regenerated composite fiber well, enhancing the interaction between these two components and endowing the composite fiber having a 20% CNT loading with an excellent mechanical strength of 185 MPa. Benefiting from the formation of conductive paths, the composite fiber with the diameter of about 50 μm possessed an electrical conductivity value in the range of 64–1274 S/m for 5–20 wt% CNT loading. This excellent mechanical strength and high electrical conductivity enable the composite fiber to exhibit a great potential in joule heating; the heating temperature of cellulose/CNT-20 fiber reached more than 55 °C within 15 s at 9 V. In addition, the multifunctional filaments are further manufactured as a water sensor to measure humidity. This work provides a potential material that can be applied in the fields of wearable electronics and smart flexible fabrics.
Expansion in the supply of intermittent renewable energy sources on the electricity grid can potentially benefit from implementation of large-scale compressed air energy storage in porous media systems (PM-CAES) such as aquifers and depleted hydrocarbon reservoirs. Despite a large government research program 30 years ago that included a test of air injection and production in an aquifer, and an abundance of literature on CAES mostly relevant to caverns, there remain fundamental questions about the hydrologic and energetic performance of PM-CAES. We have developed rigorous simulation capabilities for PM-CAES that include modeling the coupled wellbore–reservoir system. Through consideration of a prototypical PM-CAES wellbore–reservoir system representing a depleted hydrocarbon reservoir, we have simulated 100 daily cycles of PM-CAES. We find that (1) PM-CAES can store energy but that pervasive pressure gradients in PM-CAES result in spatially variable energy storage density in the reservoir, (2) the wellbore–reservoir storage component of PM-CAES is very efficient, (3) cap-rock and hydrologic seals along with proper sizing of the PM-CAES reservoir prevent excess pressure diffusion from being a problem, and (4) injection and production of air does not significantly mobilize residual liquid water in the reservoir. 相似文献
A gas chromatography–mass spectrometry assay was developed and validated for the simultaneous determination of phthalates and adipates in human serum. The phthalates and adipates studied were dimethyl phthalate, diethyl phthalate, dibutyl phthalate, benzylbutyl phthalate, di‐2‐ethylhexyl phthalate, di‐n‐octyl phthalate, diethyl adipate, dibutyl adipate, diisobutyl adipate, bis(2‐butoxyethyl) adipate and di‐2‐ethylhexyl adipate, with diisooctyl phthalate as internal standard. The extraction and cleaning up procedure was carried out with solid‐phase extraction cartridges containing dimethyl butylamine groups, which showed extraction efficiencies over 88% for each analyte and the internal standard. The calibration curves obtained were linear with correlation coefficients greater than 0.98. For all analytes, the assay gave CV% values for intra‐day precision from 4.9 to 13.3% and mean accuracy values from 91.4 to 108.4%, while inter‐day precision was 5.2–13.4% and mean accuracy 91.0–110.2%. The limits of detection for the assay of phthalates and adipates were in the range 0.7–4.5 ng/mL. The method is simple, sensitive and accurate, and allows for simultaneous determination of nanogram levels of phthalates and adipates in human serum. It was successfully applied to an investigation on the level of phthalates and adipates in a non‐occupationally exposed population. 相似文献
A magnetoelectrically permeable interface crack between two semi-infinite magnetoelectroelastic planes under the action of a heat flow and remote magnetoelectromechanical loadings is considered, where the assumption of frictionless contact between two dissimilar halfplanes is adopted. Not only the solutions of the interface crack problem are presented in an explicit form, but also the general condition for the transition from a perfect thermal contact of two magnetoelectroelastic bodies to their separation is given. 相似文献
The combination of N,N-dibromo-p-tolunesulfonamide (4-TsNBr2) and TsNH2 was found to be an efficient halogen/nitrogen source for the aminohalogenation of β-methyl-β-nitrostyrenes with manganese (II) acetate as the catalyst in the presence of 4 Å molecular sieves. The reaction results in vicinal bromoamino nitroalkanes with the opposite regioselectivity comparing with those reported, which was also confirmed by X-ray structural analysis. 相似文献
Carbon black (CB) without micropores was functionalized by mixed acid and used to explore the surface chemistry effect on the production of hydrogen peroxide (H2O2). The CB materials were characterized by N2 adsorption‐desorption, XRD, SEM, FTIR, and TPD. The results of different characterization methods indicated that both the textural features and the surface chemical properties of CB were significantly modified by the acidic treatment. The catalytic performance of the modified CBs for hydroxylamine (NH2OH) oxidation increased with increasing the surface oxygen‐containing species. The yield of H2O2 approached 30% with the corresponding concentration of 73.9 mmol·L?1 (w=0.25%) over the most promising CB catalyst, which was much superior to the results obtained on supported noble metals. Correlations between catalytic activity and concentration of different surface functional groups on the CB samples confirmed that the quinonoid species might be the active species. 相似文献