Genotypic Variability Among Soybean Genotypes Under NaCl Stress and Proteome Analysis of Salt-Tolerant Genotype

The present investigation was conducted to evaluate salt tolerance in ten genotypes of soybean (Glycine max L.). Twelve-day-old seedlings, grown hydroponically, were treated with 0, 25, 50, 75, 100, 125 and 150?mM NaCl for 10?days. Growth, lipid peroxidation and antioxidant enzyme activities were evaluated. Growth, measured in terms of length, fresh weight and dry weight of plants, was drastically reduced in Pusa-24 while there was little effect of NaCl treatment on Pusa-37 genotype of soybean. High level of lipid peroxidation was observed in Pusa-24 as indicated by increased level of malondialdehyde. Activities of superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase were maximum in Pusa-37 where 9-, 1-, 5- and 6-fold increase over control were observed, respectively. The results suggested that Pusa-24 and Pusa-37 are salt-sensitive and salt-tolerant genotype of soybean, respectively, and antioxidant defence system is involved in conferring the sensitiveness and tolerance in these genotypes. Salt-tolerant genotype Pusa-37, was further analysed by 2-dimensional gel electrophoresis to analyse the differential expression of proteins at high salt stress. In the present study, 173 protein spots were identified. Of these, 40 proteins were responsive to salinity in that they were either up- or downregulated. This study could help us in identifying the possible regulatory switches (gene/s) controlling novel proteins of the salt-tolerant genotype of the crop plants and their possible role in defence mechanism.     

On representations distinguished by unitary groups

Let E/F be a quadratic extension of number fields. We study periods and regularized periods of cusp forms and Eisenstein series on $\operatorname {GL}_{n}( \mathbf {A}_{E})$ over a unitary group of a Hermitian form with respect to E/F. We provide factorization for these periods into locally defined functionals, express these factors in terms of suitably defined local periods and characterize global distinction. We also study in detail the analogous local question and analyze the space of invariant linear forms under a unitary group.     

Optimum vibration absorber (tuned mass damper) design for linear damped systems subjected to random loads

Optimum design of dynamic vibration absorbers (DVAs) installed on linear damped systems that are subjected to random loads is studied and closed-form design formulas are provided. Three cases are considered in the optimization process: Minimizing the variance of the displacement, velocity and acceleration of the main mass. Exact optimum design parameters for the velocity case, which to the best knowledge of the author do not exist in the literature, are derived for the first time. Exact solutions are found to be directly applicable for practical use with no simplification needed. For displacement and acceleration cases, a solution for the optimum absorber frequency ratio is obtained as a function of optimum absorber damping ratio. Numerical simulations indicate that optimum absorber damping ratio is not significantly related to the structural damping, especially when the displacement variance is minimized. Therefore, optimum damping ratio derived for undamped systems is proposed for damped systems for the displacement case. When acceleration variance is minimized, however, the optimum damping ratio derived for undamped systems is found not as accurate for damped systems. Therefore, a more accurate approximate expression is derived. Numerical comparisons with published approximate expressions at the same level of complexity indicated that proposed design formula yield more accurate estimates. Another important finding of the paper is that for specific applications where all of the response parameters are desired to be minimized simultaneously, DVAs designed per velocity criteria provide the best overall performance with the least complexity in the design equations.     

CO2 Injection into Saline Carbonate Aquifer Formations II: Comparison of Numerical Simulations to Experiments

Sequestration of carbon dioxide in geological formations is an alternative way of managing extra carbon. Although there are a number of mathematical modeling studies related to this subject, experimental studies are limited and most studies focus on injection into sandstone reservoirs as opposed to carbonate ones. This study describes a fully coupled geochemical compositional equation-of-state compositional simulator (STARS) for the simulation of CO₂ storage in saline aquifers. STARS models physical phenomena including (1) thermodynamics of sub- and supercritical CO₂, and PVT properties of mixtures of CO₂ with other fluids, including (saline) water; (2) fluid mechanics of single and multiphase flow when CO₂ is injected into aquifers; (3) coupled hydrochemical effects due to interactions between CO₂, reservoir fluids, and primary mineral assemblages; and (4) coupled hydromechanical effects, such as porosity and permeability change due to the aforementioned blocking of pores by carbonate particles and increased fluid pressures from CO₂ injection. Matching computerized tomography monitored laboratory experiments showed the uses of the simulation model. In the simulations dissolution and deposition of calcite as well as adsorption of CO₂ that showed the migration of CO₂ and the dissociation of CO₂ into HCO₃ and its subsequent conversion into carbonate minerals were considered. It was observed that solubility and hydrodynamic storage of CO₂ is larger compared to mineral trapping.     

Fabrication of PEGylated Chitosan Nanoparticles Containing Tenofovir Alafenamide: Synthesis and Characterization

Tenofovir alafenamide (TAF) is an antiretroviral (ARV) drug that is used for the management and prevention of human immunodeficiency virus (HIV). The clinical availability of ARV delivery systems that provide long-lasting protection against HIV transmission is lacking. There is a dire need to formulate nanocarrier systems that can help in revolutionizing the way to fight against HIV/AIDS. Here, we aimed to synthesize a polymer using chitosan and polyethylene glycol (PEG) by the PEGylation of chitosan at the hydroxyl group. After successful modification and confirmation by FTIR, XRD, and SEM, TAF-loaded PEGylated chitosan nanoparticles were prepared and analyzed for their particle size, zeta potential, morphology, crystallinity, chemical interactions, entrapment efficacy, drug loading, in vitro drug release, and release kinetic modeling. The fabricated nanoparticles were found to be in a nanosized range (219.6 nm), with ~90% entrapment efficacy, ~14% drug loading, and a spherical uniform distribution. The FTIR analysis confirmed the successful synthesis of PEGylated chitosan and nanoparticles. The in vitro analysis showed ~60% of the drug was released from the PEGylated polymeric reservoir system within 48 h at pH 7.4. The drug release kinetics were depicted by the Korsmeyer–Peppas release model with thermodynamically nonspontaneous drug release. Conclusively, PEGylated chitosan has the potential to deliver TAF from a nanocarrier system, and in the future, cytotoxicity and in vivo studies can be performed to further authenticate the synthesized polymer.     

Conformable variational iteration method,conformable fractional reduced differential transform method and conformable homotopy analysis method for non-linear fractional partial differential equations

In this paper, we introduce conformable variational iteration method (C-VIM), conformable fractional reduced differential transform method (CFRDTM) and conformable homotopy analysis method (C-HAM). Between these methods, the C-VIM is introduced for the first time for fractional partial differential equations (FPDEs). These methods are new versions of well-known VIM, RDTM and HAM. In addition, above-mentioned techniques are based on new defined conformable fractional derivative to solve linear and non-linear conformable FPDEs. Firstly, we present some basic definitions and general algorithm for proposal methods to solve linear and non-linear FPDEs. Secondly, to understand better, the presented new methods are supported by some examples. Finally, the obtained results are illustrated by the aid of graphics and the tables. The applications show that these new techniques C-VIM, CFRDTM and C-HAM are extremely reliable and highly accurate and it provides a significant improvement in solving linear and non-linear FPDEs.     

Triply-Logarithmic Parallel Upper and Lower Bounds for Minimum and Range Minima over Small Domains

We consider the problem of computing the minimum ofnvalues, and several well-known generalizations [prefix minima, range minima, and all nearest smaller values (ANSV)] for input elements drawn from the integer domain [1···s], wheres ≥ n. In this article we give simple and efficient algorithms for all of the preceding problems. These algorithms all takeO(log log log s) time using an optimal number of processors andO(ns^ε) space (for constant ε < 1) on the COMMON CRCW PRAM. The best known upper bounds for the range minima and ANSV problems were previouslyO(log log n) (using algorithms for unbounded domains). For the prefix minima and for the minimum problems, the improvement is with regard to the model of computation. We also prove a lower bound of Ω(log log n) for domain sizes = 2^{Ω(log n log log n)}. Since, forsat the lower end of this range, log log n = Ω(log log log s), this demonstrates that any algorithm running ino(log log log s) time must restrict the range ofson which it works.     

Synthesis and characterization of thermally stable and flame retardant hexakis(4-aminophenoxy)cyclotriphosphazene-based polyimide matrices

A new approach to the preparation of hexakis(4-aminophenoxy)cyclotriphosphazene (HACTP) based polyimide (PI) matrices is proposed, for improved thermal and flame retardant properties. HACTP was synthesized with good yield. The structure of HACTP was confirmed by various characterization techniques, such as FTIR, NMR, and mass Spectroscopy. Polyimide matrices were prepared by thermal imidization process using HACTP and dianhydrides using N-methyl pyrrolidone as a solvent. The successful formation of HACTP-based polyimide matrices was confirmed by the FTIR. Thermal properties of the PI were analyzed using DSC and TGA techniques. Data obtained from the thermal studies indicate that the HACTP-based PI possesses better thermal and flame retardant properties.     

Phytochemical Profiling and Evaluation of the Hepatoprotective Effect of Cuscuta Campestris by High-Performance Liquid Chromatography with Diode Array Detection

Plant-derived antioxidant compounds have the potential to prevent cell damage caused by free radicals. As a holoparasitic plant, Cuscuta campestris Yuncker is being valorized for treatment of liver injury and cancer prevention in traditional medicine. The main purpose of this present study is to elucidate the antioxidant- and anticancer-associated contents of C. campestris by spectroscopic and chromatographic methods. Diethyl ether, ethyl acetate, methanol, n-butanol, and water were used as extraction solvents to reach a wide range of secondary metabolites synthesized by this plant. Antioxidant potentials of these extracts were characterized by (2,2-diphenyl-1-picrylhydrazyl)-free radical scavenging activity. Their anticancer activities were evaluated on SNU-398 hepatocellular carcinoma cells and controlled on the normal adult human dermal fibroblasts (hDFs) cells. Their phenolic compounds were analyzed by high-performance liquid chromatography with diode array detector to illuminate the responsible anticancer agent(s). The ethyl acetate extract revealed the most significant antioxidant effect. Methanol and ethyl acetate extracts were found to be cytotoxic on the SNU-398 cell lines with CC₅₀ values of 18.7 and 19.6?µg?mL^?1, respectively, whereas these extracts were not cytotoxic to the adult hDFs cells. Due to their abundance in the extracts, isorhamnetin, kaempferol, and quercetin may have caused this anticancer activity. Methanol extract has the highest concentration of phenolic compounds. Based on chromatographic analyses, we propose that these anticancer effects were positively correlated with plant phenolic compounds. The results showed that this plant is worth further study for its therapeutic uses.