Vertebrate collagenase inhibitor. II. Tetrapeptidyl hydroxamic acids.

To develop a potent and specific collagenase inhibitor, a series of tetrapeptidyl hydroxamic acids were synthesized, based on the previous findings with tripeptidyl derivatives (Chem. Pharm. Bull., 38, 1007-1011, 1990). Among the series of tetrapeptidyl derivatives synthesized, R-Gly-Pro-Leu-Ala-NHOH and R-Gly-Pro-D-Leu-D-Ala-NHOH were found to be highly specific and potent inhibitors against vertebrate collagenase with an IC50 of 10(-6) M order, where R stands for Boc or acyl group. Analysis of their structure-activity relationships showed a characteristic feature of the substrate-binding site of collagenase as follows: 1) the S1 subsite forms a shallow hydrophobic pocket, although glycine residue corresponds to the subsite of the natural collagen substrate: 2) the S2 subsite constitutes a bulky pocket with less requirement for hydrophobicity: 3) the S3 subsite preferentially accommodates Pro residue: and 4) the accommodation of the P4-P1 subsites of peptidyl collagenase inhibitor to the S4-S1 subsites is required to form a tight binding of its hydroxamic acid moiety to the zinc ion at the catalytic site of the enzyme. The introduction of an enantiometric dipeptide unit, D-Leu-D-Ala, to the P2-P1 subsites demonstrated an increased binding capacity to the extended S4-S1 subsites of collagenase, thus providing proteinase-resistant inhibitor.     

Sensitive measurement of methylene blue active substances by attenuated total reflection spectrometry with a trimethylsilane-modified glass slab optical waveguide

Attenuated total reflection spectrometry with a slab optical waveguide (SOWG) was explored for the simple, rapid and sensitive measurement of total anionic surfactants by the methylene blue active substance (MBAS) method. A fused-silica sheet used as a guiding layer was modified with trimethylsilane (TMS) to extract and concentrate the MBASs on the SOWG surface. Based on preliminary studies of the adsorption behavior and visible ATR spectrum of MB on the modified silica surface, a detection wavelength of 600 nm was chosen for the sensitive measurement of anionic surfactants. When the concentration of MB was set at 10 microM in the final measurement solution, the calibration curve for a typical anionic surfactant (sodium dodecylbenzenesulfonate) was linear up to 0.6 microM and the detection limit was 0.07 microM. The proposed method was applied to the determination of total anionic surfactants in river water.     

Laser-induced fluorescence microscopic system using an optical parametric oscillator for tunable detection in microchip analysis

A laser-induced fluorescence microscopic system based on optical parametric oscillation has been constructed as a tunable detector for microchip analysis. The detection limit of sulforhodamine B (Ex. 520 nm, Em. 570 nm) was 0.2 mol, which was approximately eight orders of magnitude better than with a conventional fluorophotometer. The system was applied to the determination of fluorescence-labeled DNA (Ex. 494 nm, Em. 519 nm) in a microchannel and the detection limit reached a single molecule. These results showed the feasibility of this system as a highly sensitive and tunable fluorescence detector for microchip analysis.     

Free boson realization of

We construct a realization of the quantum affine algebra of an arbitrary level k in terms of free boson fields. In the q1 limit this realization becomes the Wakimoto realization of. The screening currents and the vertex operators (primary fields) are also constructed; the former commutes with modulo total difference, and the latter creates the highest weight state from the vacuum state of the boson Fock space.fellow of Soryushi ShogakukaiAddress after June 1: Department of Physics, Faculty of Liberal Arts, Shinshu University, Matsumoto 390, Japan     

Free Field Construction for the ABF Models in Regime II

The Wakimoto construction for the quantum affine algebra U $_q$ ( $(\widehat{\mathfrak{s}\mathfrak{l}_2 })$ ) admits a reduction to the q-deformed parafermion algebras. We interpret the latter theory as a free field realization of the Andrews–Baxter–Forrester models in regime II. We give multi-particle form factors of some local operators on the lattice and compute their scaling limit, where the models are described by a massive field theory with $\mathbb{Z}$ $_k$ symmetric minimal scattering matrices.     

Characteristics of a liquid/liquid optical waveguide using sheath flow and its application to detect molecules at a liquid/liquid interface.

The formation conditions and characteristics of a liquid/liquid optical waveguide (LLW) were studied using a two-phase sheath flow, where the inner organic phase flow acted as the core and the outer aqueous flow acted as the clad. In immiscible solvent systems, i.e., toluene/water and diethyl ether/water systems, the LLWs were formed in the range of higher than ca. 600 of the Reynolds number (Re), where the linear velocity of the organic solvent was much higher than that of the aqueous solution. On the other hand, in a miscible solvent system, i.e., a tetrahydrofuran/water system, a stable LLW was formed in the range of a much lower Re than in immiscible systems. Moreover, the molecules at the toluene/water interface of the LLW were observed with both fluorescence and absorbance measurement systems. In particular, the change in the fluorescence spectrum of 1-anilino-8-naphthalenesulfonate (ANS) at the interface within 1 ms was observed by this method, indicating the usefulness of the LLW for a fast kinetic study of a liquid/liquid interface.     

Micromachining and surface processing of the super-hard nano-polycrystalline diamond by three types of pulsed lasers

Laser beam micromachining was applied to super-hard nano-polycrystalline diamond (NPD) synthesized by the direct conversion of graphite at high pressure and high temperature. Three types of pulsed lasers were tested: nanosecond near-infrared, nanosecond near-ultraviolet, and femtosecond near-infrared lasers. The latter two were also applied for synthetic single crystal of diamond to compare the results with those of the NPD. It was demonstrated that the nanosecond near-infrared laser was the most efficient device for rough shaping of the NPD, while the ultraviolet and femtosecond lasers give satisfactory results for precise surface finishing of it. The properties of the laser-processed surfaces were analyzed by scanning and transmission electron microscopy, laser scanning microscopy, and micro Raman spectroscopy. These analyses demonstrated that the three types of lasers play different and complementary roles, and that their combination is the best suitable solution for micromachining of the hardest diamond into any desired shapes.     

Infinitely many shape invariant discrete quantum mechanical systems and new exceptional orthogonal polynomials related to the Wilson and Askey–Wilson polynomials

Two sets of infinitely many exceptional orthogonal polynomials related to the Wilson and Askey–Wilson polynomials are presented. They are derived as the eigenfunctions of shape invariant and thus exactly solvable quantum mechanical Hamiltonians, which are deformations of those for the Wilson and Askey–Wilson polynomials in terms of a degree ?   (?=1,2,…$?=1,2,\dots$) eigenpolynomial. These polynomials are exceptional in the sense that they start from degree ??1$??1$ and thus not constrained by any generalisation of Bochner's theorem.     

Quasifinite highest weight modules over the superW 1+∞algebra

We study quasifinite highest weight modules over the supersymmetric extension of theW ₁₊ algebra on the basis of the analysis by Kac and Radul. We find that the quasifiniteness of the modules is again characterized by polynomials, and obtain the differential equations for highest weights. The spectral flow, free field realization over the (B, C)-system, and the embedding into (|) are also presented.Address after April 1, 1994: Research Institute for Mathematical Sciences, Kyoto University, Kyoto 606, JapanAddress after April 1, 1994: Uji Research Center, Yukawa Institute for Theoretical Physics, Kyoto University, Uji 611, Japan     

Reflection K-matrices of the 19-vertex model and XXZ spin-1 chain with general boundary terms

We derive and classify all solutions of the boundary Yang-Baxter equation (or the reflection equation) for the 19-vertex model associated with . An integrable XXZ spin-1 chain Hamiltonian with general boundary interactions is also obtained.