Preparation,mechanical properties,and in vitro biocompatibility of novel nanocomposites based on polyhexamethylene carbonate fumarate and nanohydroxyapatite

In the current study a new biodegradable nanocomposite based on poly hexamethylene carbonate fumarate (PHMCF) and nano‐sized hydroxyapatite (nano‐HA) has been developed. A silane coupling agent γ‐methacryloxypropyltrimethoxy silane, was used to achieve a good interfacial adhesion between nano‐HA and PHMCF matrix. PHMCF with different nano‐HA contents were characterized using dynamical mechanical thermal analysis (DMTA) and hardness test. The effect of frequency on storage modulus, glass transition temperature (T_g) and the damping were investigated. In vitro cytotoxicity and proliferation were performed using G292 cell lines by MTT assay. The addition of nano‐HA resulted in an increment on the storage modulus and decrement on the damping. Along with improvement in mechanical properties of composites, the addition of nano‐HA resulted in enhanced cell proliferation. Following these results, the newly developed nano‐PHMCF composite scaffold may be considered for bone tissue engineering applications. Copyright © 2009 John Wiley & Sons, Ltd.     

Molecular mechanochemistry: low force switch slows enzymatic cleavage of human type I collagen monomer

In vertebrate animals, fibrillar collagen accumulates, organizes, and persists in structures which resist mechanical force. This antidissipative behavior is possibly due to a mechanochemical force-switch which converts collagen from enzyme-susceptible to enzyme-resistant. Degradation experiments on native tissue and reconstituted fibrils suggest that collagen/enzyme kinetics favor the retention of loaded collagen. We used a massively parallel, single molecule, mechanochemical reaction assay to demonstrate that the effect is derivative of molecular mechanics. Tensile loads higher than 3 pN dramatically reduced (10×) the enzymatic degradation rate of recombinant human type I collagen monomers by Clostridium histolyticum compared to unloaded controls. Because bacterial collagenase accesses collagen at multiple sites and is an aggressive cleaver of the collagen triple helical domain, the results suggest that collagen molecular architecture is generally more stable when mechanically strained in tension. Thus the tensile mechanical state of collagen monomers is likely to be correlated to their longevity in tissues. Further, strain-actuated molecular stability of collagen may constitute the fundamental basis of a smart structural mechanism which enhances the ability of animals to place, retain, and load-optimize material in the path of mechanical forces.     

Choosing the optimal intervention method to reduce human-related machine failures

This paper presents a novel method to quantify the effects of human-related factors on the risk of failure in manufacturing industries. When failures can be caused by operators, the decision maker must intervene to mitigate operator-related risk. There are numerous intervention methods possible; we develop a revenue model that provides the decision-maker with a systematic tool to perform a cost-benefit analysis, balancing the advantage of risk reduction, against the direct cost of the intervention method.     

Effects of operator learning on production output: a Markov chain approach

We develop a Markov chain approach to forecast the production output of a human-machine system, while encompassing the effects of operator learning. This approach captures two possible effects of learning: increased production rate and reduced downtime due to human error. In the proposed Markov chain, three scenarios are possible for the machine at each time interval: survival, failure, and repair. To calculate the state transition probabilities, we use a proportional hazards model to calculate the hazard rate, in terms of operator-related factors and machine working age. Given the operator learning curves and their effect on reducing human error over time, the proposed approach is considered to be a non-homogeneous Markov chain. Its result is the expected machine uptime. This quantity, along with production forecasting at various operator skill levels, provides us with the expected production output.     

Double-diffusivity heat generation effects on bioconvection process embedded in a vertical porous surface with variable fluid properties

Journal of Thermal Analysis and Calorimetry - The present paper concerns the bioconvective flow, mass and heat transfer including motile microorganisms on a vertical surface saturated with porous...     

Comparative study of mixed convection heat transfer of water–Cu nanofluid in an enclosure having multiple rotating circular cylinders with different configurations and considering harmonic cylinders rotation

Journal of Thermal Analysis and Calorimetry - In the present study, heat transfer in a lid-driven square cavity in the existence of four rotating cylinders having harmonic motion was investigated...     

Effective field theory for massive gravitons and gravity in theory space

We introduce a technique for restoring general coordinate invariance into theories where it is explicitly broken. This is the analog for gravity of the Callan-Coleman-Wess-Zumino formalism for gauge theories. We use this to elucidate the properties of interacting massless and massive gravitons. For a single graviton with a Planck scale M_Pl and a mass m_g, we find that there is a sensible effective field theory which is valid up to a high-energy cutoff Λ parametrically above m_g. Our methods allow for a transparent understanding of the many peculiarities associated with massive gravitons, among them the need for the Fierz-Pauli form of the Lagrangian, the presence or absence of the van Dam-Veltman-Zakharov discontinuity in general backgrounds, and the onset of non-linear effects and the breakdown of the effective theory at large distances from heavy sources. The natural sizes of all non-linear corrections beyond the Fierz-Pauli term are easily determined. The cutoff scales as Λ∼(m_g⁴M_Pl)^1/5 for the Fierz-Pauli theory, but can be raised to Λ∼(m_g²M_Pl)^1/3 in certain non-linear extensions. Having established that these models make sense as effective theories, there are a number of new avenues for exploration, including model building with gravity in theory space and constructing gravitational dimensions.     

Synthesis,cytotoxic assessment,and molecular docking studies of 2,6-diaryl-substituted pyridine and 3,4- dihydropyrimidine-2(1H)-one scaffolds

Cancer is one of the main global health problems. In order to develop novel antitumor agents, we synthesized 3,4-dihydropyrimidine-2(1H)-one (DHPM) and 2,6-diaryl-substituted pyridine derivatives as potential antitumor structures and evaluated their cytotoxic effects against several cancer cell lines. An easy and convenient method is reported for the synthesis of these derivatives, employing cobalt ferrite (CoFe ₂ O ₄ @SiO ₂ -SO ₃ H) magnetic nanoparticles under microwave irradiation and solvent-free conditions. The structural characteristics of the prepared nanocatalyst were investigated by FTIR, XRD, SEM, and TGA techniques. In vitro cytotoxic effects of the synthesized products were assessed against the human breast adenocarcinoma cell line (MCF-7), gastric adenocarcinoma (AGS), and human embryonic kidney (HEK293) cells via MTT assay. The results indicated that compound 4r (DHPM derivative) was the most toxic molecule against the MCF-7 cell line (IC ₅₀ of 0.17 μg/mL). Moreover, compounds 4j and 4r (DHPM derivatives) showed excellent cytotoxic activities against the AGS cell line, with an IC ₅₀ of 4.90 and 4.97 μg/mL, respectively. Although they are pyridine derivatives, compounds 5g and 5m were more active against the MCF-7 cell line. Results showed that the candidate compounds exhibited low cytotoxicity against HEK293 cells. The kinesin Eg5 inhibitory potential of the candidate compounds was evaluated by molecular docking. The docking results showed that, among the pyridine derivatives, compound 5m had the most free energy of binding (–9.52 kcal/mol) and lowest Ki (0.105 μM), and among the pyrimidine derivatives, compound 4r had the most free energy of binding (–7.67 kcal/mol) and lowest Ki (2.39 μM). Ligand-enzyme affinity maps showed that compounds 4r and 5m had the potential to interact with the Eg5 binding site via H-bond interactions to GLU116 and GLY117 residues. The results of our study strongly suggest that DHPM and pyridine derivatives inhibit important tumorigenic features of breast and gastric cancer cells. Our results may be helpful in the further design of DHPMs and pyridine derivatives as potential anticancer agents.