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111.
The plasma-primed seeds of Catharanthus roseus were cultured in a hormone-free culture medium under sterile conditions. Plasma of 30 or 60 s improved root length (mean = 41.4%) and biomass (mean = 47%), whereas plasma of 90 s delayed plant growth. The plasma treatments enhanced concentrations of photosynthetic pigments and soluble phenols. Plasma of 90 s increased the proline level. With a similar trend, plasma priming induced activities of phenylalanine ammonia-lyase, catalase (about twofold) and peroxidase (31%) enzymes. Plasma also upregulated the expressions of deacetylvindoline O-acetyltransferase gene by an average of 7.8 times. Similarly, the plasma-treated seedlings contained higher concentrations of alkaloids (mean = 72%). Here, molecular evidence is provided on the plasma-associated modifications in secondary metabolism.  相似文献   
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The need for effective and efficient methods for pathogen detection in water is as serious as ever due to the health risk posed to human population by the consumption of pathogen-contaminated water. One of the important research streams which have been focused on by researchers for development of novel techniques for this purpose is biosensor technologies. Using different bio-recognition elements and transduction methodologies, biosensors have the potential to detect their analyte of interest in a fast and highly specific manner. Different pathogenic agents can be recognised by toll-like receptors (TLRs). The innate immune system of higher organisms employs TLRs for triggering intracellular signalling and induction of the expression of immune response genes. In this report, we explore the challenges associated with employing TLRs for pathogen detection in water samples. Although methods using TLR expressing cells also have been discussed, the focus of this review is on using TLR proteins as the bio-recognition elements in biosensors.  相似文献   
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The spiro-compound 3′H-spiro[indole-3,2′-[1,3]benzothiazole-2(1H)-one (IBTH2) was synthesized and its structure was determined using spectroscopic techniques (FTIR, 1H NMR and mass) and X-ray crystallography. This ligand possesses different centers for coordination. Reactions of [M(CO)6], M = Cr, Mo or W with IBTH2 in THF under reduced pressure were studied. For chromium a complex with molecular formula [Cr(ITP)2] was isolated; where ITP is the opened form of the ligand which occurred through Cspiro–S bond, while [Mo(CO)5(IBTH2)] and [W(CO)5(IBTH2)] were isolated from the reaction of IBTH2 with molybdenum and tungsten carbonyls, respectively. All complexes were characterized by elemental analysis, IR, mass and 1H NMR spectroscopy. The biological activity of the ligand and its complexes were studied and compared with the parent compound isatin.  相似文献   
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In this work, we are concerned with a general class of abstract semilinear autonomous functional differential equations with a non-dense domain on a Banach space. Our objective is to study, using the Crandall-Liggett approach, the solutions as a semigroup of non-linear operators.  相似文献   
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