Fluorescence Polarization as a Functional Parameter in Monitoring Living Cells: Theory and Practice

The use of fluorescence polarization as a functional parameter in monitoring cellular activation calls for the reliable and accurate measurement of the fluorescence intensity and polarization (FI and FP) of microscopic objects. The relevant experimental parameters that enter such measurements are thoroughly discussed. The possibility of executing FP measurements properly by flow-through systems is compared with that of static cytometry. Remarks on the effects of high-power excitation on markers and cells conclude the paper.     

On the extreme points of the polytope of symmetric matrices with given row sums

The extreme points of the convex polytope of nonnegative symmetric matrices of order n with prescribed row sums are fully characterized by their respective graphs. For infinite matrices such a characterization is shown to be impossible. However, after imposing some additional conditions on the positive entries of the matrices, a considerable subfamily of infinite matrices is characterized by its graphs.     

Perturbative and non-perturbative two component model of deep inelastic scattering

We propose a two-component model for describing deep inelastic scattering, based on nonperturbative and perturbative mechanisms. The nonperturbative dynamics obey the usualS-matrix constraints, as realized by the Dual Topological Unitarization Scheme, whereas the perturbative component stems from the underlying field theory. However, these two components arenot independent.     

On maximal circuits in directed graphs

An exact bound is obtained for the number of edges in a directed graph which ensures the existence of a circuit exceeding a prescribed length.Another proof of an analogous result of Erdös and Gallai for undirected graphs is supplied in the Appendix.     

On the role of production mechanisms in rising cross sections

The interplay among the various mechanisms in generating a rise in cross sections is discussed in the framework of the recently proposed s-channel absorption equation. Our approach simultaneously controls the energy behaviour of the diffractive and non-diffractive components.     

Spectroscopy of C

The ¹⁴C(t, p)¹⁶C reaction locates five new states in ¹⁶C, at excitation energies of 3120 ± 15, 3983 ± 10, 4136 ± 10 and 6109 ± 15 keV, in addition to the g.s. and 1.76 MeV states. The 3.02 and 3.98 MeV states appear to be the second 0⁺ and 2⁺ 2p-2h states, respectively. The 4.14 MeV state has J^π = 4⁺ and the 6.11 MeV state has J^π = 2⁺, 3⁻, or 4⁺.     

Drop impact on surfactant films and solutions

Drops impacting on horizontal aqueous surfactant films have been analyzed using a high-speed camera. Drops of either water or aqueous surfactant solutions had a diameter of 2.4?±?0.4 mm and impacted with a velocity of 0.1 to 1.3 m/s. As surfactants, anionic sodium dodecyl sulfate and cationic cetyltrimethyl ammonium bromide were used. Pure water drops impacting on freestanding surfactant films showed coalescence, bouncing, partial bouncing, passing, and partial passing. For bouncing, the concentration of surfactant in the surfactant film must exceed the critical micelle concentration. When surfactant was added to the drop, coalescence and partial passing were suppressed. We attribute the different behavior to different hydrodynamic boundary conditions at the surface of pure water and surfactant solution, leading to different repulsive hydrodynamic forces arising when the air has to flow out of the closing gap between the two liquid surfaces. The boundary condition changes as a function of surfactant concentration from a slip to no-slip, leading to stronger hydrodynamic repulsion. In addition, estimates of the characteristic velocities show that diffusion of air into the water is slow and can only account for the very last thinning of the air gap before coalescence.