Multilayer Microcapsules for FRET Analysis and Two‐Photon‐Activated Photodynamic Therapy

Microcapsules obtained by layer‐by‐layer assembly provide a good platform for biological analysis owing to their component diversity, multiple binding sites, and controllable wall thickness. Herein, different assembly species were obtained from two‐photon dyes and traditional photosensitizers, and further assembled into microcapsules. Fluorescence resonance energy transfer (FRET) was shown to occur between the two‐photon dyes and photosensitizers. Confocal laser scanning microscopy (CLSM) with one‐ and two‐photon lasers, fluorescence lifetime imaging microscopy (FLIM), and time‐resolved fluorescence spectroscopy were used to analyze the FRET effects in the microcapsules. The FRET efficiency could easily be controlled through changing the assembly sequence. Furthermore, the capsules are phototoxic upon one‐ or two‐photon excitation. These materials are thus expected to be applicable in two‐photon‐activated photodynamic therapy for deep‐tissue treatment.     

Autocrine‐Based Selection of Drugs That Target Ion Channels from Combinatorial Venom Peptide Libraries

Animal venoms represent a rich source of pharmacologically active peptides that interact with ion channels. However, a challenge to discovering drugs remains because of the slow pace at which venom peptides are discovered and refined. An efficient autocrine‐based high‐throughput selection system was developed to discover and refine venom peptides that target ion channels. The utility of this system was demonstrated by the discovery of novel Kv1.3 channel blockers from a natural venom peptide library that was formatted for autocrine‐based selection. We also engineered a Kv1.3 blocker peptide (ShK) derived from sea anemone to generate a subtype‐selective Kv1.3 blocker with a long half‐life in vivo.     

适合SBA-15介孔材料工业化生产的改良方法

在SBA-15介孔材料100L中试和2m3工业放大合成基础上,采用多次水解合并晶化、母液循环使用和以工业正硅酸乙酯(TEOS)原料为硅源三种改良方法,提高SBA-15材料的合成效率,减少废酸水排放和降低原料成本.结果表明,母液经加热蒸除乙醇至5%和工业原料TEOS经蒸除沸点低于163oC的前馏分后使用,在有效保证合成出结构性能良好SBA-15材料的同时,可使生产效率提高1倍,分别减少50%和90%三嵌段共聚物和盐酸用量,且几乎无废水排放,最终节约SBA-15原料成本约60%.这些改良方法有利于SBA-15材料工业生产时的环境保护和成本控制,具有很高可行性.     

Minimizers for the embedding of Besov spaces

Using the profile decomposition, we will show the relatively compactness of the minimizing sequence to the critical embeddings between Besov spaces, which implies the existence of minimizer of the critical embeddings of Besov spaces $\dot{B}^{s_1}_{p_1,q_1}\hookrightarrow \dot{B}^{s_2}_{p_2,q_2}$ in $d$ dimensions with $s_1-d/p_1=s_2-d/p_2$, $s_1>s_2$ and $1 \leq q_1相似文献   

《高等数学》中的“爱尔兰根纲领”及其应用

"爱尔兰根纲领"是几何学史上一篇划时代的文献,它提出的"变换下的几何不变量"思想对几何、代数乃至其后整个数学的发展都产生了广泛而深刻的影响.然而,这一重要思想在高等数学中的体现和应用却鲜为人注意.为此,本文详细探讨了"爱尔兰根纲领"的思想在高等数学内容中的体现以及它在高等数学中的应用.     

Hydrogen Bonding Assembled 3D Supramolecular Structures Formed by 5-Amino-2,4,6-triiodoisophthalic Acid and N-Heterocyclic Aromatic Ligands

Three salts constructed by 5-amino-2,4,6-triiodoisophthalic acid(ATIPA) with N-heterocycles aromatic coformers such as pyridine tetrazolium, tetramethylpyraziiie and cyanuric acid were synthesized by slowing evaporation of solvent. X-Ray single crystal analysis shows that hydrogen protons of the carboxyl groups transfer to nitrogen atoms of the N-heterocyclic coformers to form N-H…0 hydrogen bonds in all the three compounds. A huge amount of H-bonds play significant role in tlie construction of these compounds and all of them generate 3D structures through strong O-H…N, O-H…O, N-H…O and weak C-H…O hydrogen bonds. Moreover, solvent water molecules are indispensable in the formation of compounds 1 and 3, which constitutes different supramolecular synthons to bridge individual molecules and chains to form stable structures. In addition, these crystal structures were further characterized by themiogravimetric analysis and infrared spectroscopy.     

高效液相色谱法测定庆大霉素C组分的不同检测方式测定结果的比较

各国药典中关于庆大霉素C组分的测定方法均为高效液相色谱法,但检测方式及分离效果不同。为此采用直观推导式演进特征投影法(HELP),对高效液相色谱-二极管阵列检测采集的庆大霉素C组分色谱数据进行解析,分辨出各物质的色谱曲线,在扣除了未分离的杂质峰对庆大霉素C1组分的干扰后,对柱前邻苯二醛衍生化-二极管阵列检测法及目前中国药典 2005 版收载的高效液相色谱-蒸发光散射检测方法测定庆大霉素C组分的准确性进行了比较,并运用柱切换技术,证明二者测定结果的一致性。     

甲硫氨酸-脑啡肽的活性位点

用ＡＭ１方法中计算静电势的ＰＭＥＰ子程序了甲硫氨酸－脑啡肽的活性位点。通过计算得到了整个分子的三维空间静电势分布和由静电势导出的各原子的电荷分布。进一步分析,确定模型分子活性位点为酪氨酸残在的叔氨原子和苯酚基、苯丙氨酸残基的苯基及部分氧原子。     

Structural basis of specific binding between Aurora A and TPX2 by molecular dynamics simulations

In the present study, the impacts of G198N and W128F mutations on the recognition between Aurora A and targeting protein of Xenopus kinesin-like protein 2 (TPX2) were investigated using molecular dynamics (MD) simulations, free energy calculations, and free energy decomposition analysis. The predicted binding free energy of the wild-type complex is more favorable than those of three mutants, indicating that both single and double mutations are unfavorable for the Aurora A and TPX2 binding. It is also observed that the mutations alternate the binding pattern between Aurora A and TPX2, especially the downstream of TPX2. An intramolecular hydrogen bond between the atom OD of Asp11(TPX2) and the atom HE1 of Trp34(TPX2) disappear in three mutants and thus lead to the instability of the secondary structure of TPX2. The combination of different molecular modeling techniques is an efficient way to understand how mutation has impacts on the protein-protein binding and our work gives valuable information for the future design of specific peptide inhibitors for Aurora A.