Linearisierung von Gruppendaten mit Anwendungen auf Knotengruppen

Ohne Zusammenfassung     

Flow-injection fluorimetric assay of nitrogen-containing substrates by on-line enzymatic generation of ammonia

A fluorimetric flow-injection method for the determination of nitrogen-containing substrates, which can be enzymatically degraded to ammonium/ammonia is described. The generated ammonia is detected fluorimetrically after on-line derivatization with o-phthaldialdehyde (OPA) and sulphite. The derivatization procedure is optimized by a fractional factorial design at two levels and by a super modified simplex procedure to obtain a high sensitivity and a low detection limit for ammonia in the micromolar concentration range. Under the given flow conditions the detection limit is 1 μM, and considerable selectivity for ammonia over primary amines such as unconverted substrate and amino acids is achieved. Two enzymatic systems, incorporating immobilized creatinine iminohydrolase (CIH) and L-aspartase column reactors, respectively, are tested as model systems. The feasibility of the CIH system for the practical assay of creatinine in serum samples is demonstrated.     

Kummer's lemma for cyclic 2-groups

For cyclic 2-groupsC, we characterize the kernel of the map induced on the units of the integral group ring by the coefficient reduction . This allows us to prove that, for any finite abelian 2-groupA, the circular units of ℤA (i.e. those which are mapped to cyclotomic units by every character ofA) can be generated in a certain systematic way. Work supported in part by an NSERC (Canada) operating grant     

Equilibrium prices supported by dual price functions in markets with non-convexities

The issue of finding market clearing prices in markets with non-convexities has had a renewed interest due to the deregulation of the electricity sector. In the day-ahead electricity market, equilibrium prices are calculated based on bids from generators and consumers. In most of the existing markets, several generation technologies are present, some of which have considerable non-convexities, such as capacity limitations and large start-up costs. In this paper we present equilibrium prices composed of a commodity price and an uplift charge. The prices are based on the generation of a separating valid inequality that supports the optimal resource allocation. In the case when the sub-problem generated as the integer variables are held fixed to their optimal values possess the integrality property, the generated prices are also supported by non-linear price functions that are the basis for integer programming duality.     

High content screening for G protein-coupled receptors using cell-based protein translocation assays

G protein-coupled receptors (GPCRs) have been one of the most productive classes of drug targets for several decades, and new technologies for GPCR-based discovery promise to keep this field active for years to come. While molecular screens for GPCR receptor agonist- and antagonist-based drugs will continue to be valuable discovery tools, the most exciting developments in the field involve cell-based assays for GPCR function. Some cell-based discovery strategies, such as the use of beta-arrestin as a surrogate marker for GPCR function, have already been reduced to practice, and have been used as valuable discovery tools for several years. The application of high content cell-based screening to GPCR discovery has opened up additional possibilities, such as direct tracking of GPCRs, G proteins and other signaling pathway components using intracellular translocation assays. These assays provide the capability to probe GPCR function at the cellular level with better resolution than has previously been possible, and offer practical strategies for more definitive selectivity evaluation and counter-screening in the early stages of drug discovery. The potential of cell-based translocation assays for GPCR discovery is described, and proof-of-concept data from a pilot screen with a CXCR4 assay are presented. This chemokine receptor is a highly relevant drug target which plays an important role in the pathogenesis of inflammatory disease and also has been shown to be a co-receptor for entry of HIV into cells as well as to play a role in metastasis of certain cancer cells.     

Tumor-associated antigens identified by mRNA expression profiling as tumor rejection epitopes

Thirteen H-2b-binding peptides derived from six potentially overexpressed proteins in p53-/- thymoma (SM7) cells were studied for immunogenecity and vaccine-induced prevention of tumor growth in mice inoculated with SM7 tumor cells. Six of the peptides generated specific CTL responses after immunization, but only two of these peptides (RAD23-31 and RAD24-31) were capable of generating a weak vaccination-induced protection against adoptive tumor growth. SM7 inoculated mice treated with a blocking antibody against the inhibitory T cell signal transducing molecule CTLA4 appeared to delay tumor take, suggesting that SM7 thymoma cells are recognized by the adaptive immune system of the host. However, prophylactic vaccination with RAD23-31 and RAD24-31 peptides combined with anti-CTLA4 Ab treatment and did not improve tumor resistance. Our data would indicate that vaccination with immunogenic peptides derived from potentially overexpressed tumor proteins, as identified by mRNA expression profiling of p53-/- thymoma cells, at best results in a weak tumor protection thus questioning this way of detection of new tumor rejection epitopes.     

Aminocyclopentanols as sugar mimics. Synthesis from unsaturated bicyclic lactones by Overman rearrangement

Bicyclic cyclopentane lactones, prepared from bromodeoxyaldonolactones, were transformed into aminocyclopentanols with an Overman rearrangement as the key step. Two of the compounds prepared, 7 and 19, were found to be good inhibitors of jack bean alpha-mannosidase and beta-D-N-acetylglucosaminidase, respectively.     

A local search heuristic for the Multi-Commodity k-splittable Maximum Flow Problem

The Multi-Commodity $k$ -splittable Maximum Flow Problem consists of maximizing the amount of flow routed through a network such that each commodity uses at most $k$ paths and such that edge capacities are satisfied. The problem is $\mathcal NP $ -hard and has application in a.o. telecommunications. In this paper, a local search heuristic for solving the problem is proposed. The heuristic is an iterative shortest path procedure on a reduced graph combined with a local search procedure to modify certain path flows and prioritize the different commodities. The heuristic is tested on benchmark instances from the literature and solves 83 % of the instances to optimality. For the remaining instances, the heuristic finds good solution values which on average are 1.04 % from the optimal. The heuristic solves all instances in less than a second. Compared to other heuristics, the proposed heuristic again shows superior performance with respect to solution quality.     

Sun exposure and Protection Behavior of Danish Farm Children: Parental Influence on Their Children

Healthy sun habits acquired in childhood could reduce skin cancer incidence. We examined the sun exposure and protection behavior of an expected high‐exposure group of children, and the association to their parents. Open, prospective cohort study. One hundred and thirty nine participants (40 families) kept daily sun behavior diaries (sun exposure, sunscreen use, sunburns) over a 4‐month summer period (15 985 diary days). The Pigment Protection Factor (PPF), an objective measure of sun exposure, was measured at two body sites, before and after summer. All participants presented data from the same 115 days. Risk behavior (sun exposure of upper body) took place on 9.5 days (boys) and 15.6 days (girls). Sunburn and sunscreen use were infrequent. Boys’ sun exposure resulted in an increased photo protection over the study period of 1.7 SED (upper arm) and 0.8 SED (shoulder) to elicit erythema. Corresponding values for girls were as follows: 0.9 SED (upper arm) and 0.5 SED (shoulder). Boys’ sunscreen use correlated to their mothers’ (r = 0.523, P = 0.02). Girls’ number of risk days (r = 0.552, P = 0.005) and sun exposure (upper arm: r = 0.621, P < 0.001) correlated to their mothers’. The children's sun exposure was substantial. Only mothers influenced children's sun behavior and exposure. This may be of relevance in future sun protection campaigns.