An Activatable Lanthanide Luminescent Probe for Time‐Gated Detection of Nitroreductase in Live Bacteria

Herein we report the development of a turn‐on lanthanide luminescent probe for time‐gated detection of nitroreductases (NTRs) in live bacteria. The probe is activated through NTR‐induced formation of the sensitizing carbostyril antenna and resulting energy transfer to the lanthanide center. This novel NTR‐responsive trigger is virtually non‐fluorescent in its inactivated form and features a large signal increase upon activation. We show that the probe is capable of selectively sensing NTR in lysates as well as in live bacteria of the ESKAPE family which are clinically highly relevant multiresistant pathogens responsible for the majority of hospital infections. The results suggest that our probe could be used to develop diagnostic tools for bacterial infections.     

Raman Spectroscopic Signature of Ectoine Conformations in Bulk Solution and Crystalline State

Recent crystallographic results revealed conformational changes of zwitterionic ectoine upon hydration. By means of confocal Raman spectroscopy and density functional theory calculations, we present a detailed study of this transformation process as part of a Fermi resonance analysis. The corresponding findings highlight that all resonant couplings are lifted upon exposure to water vapor as a consequence of molecular binding processes. The importance of the involved molecular groups for water binding and conformational changes upon hydration is discussed. Our approach further shows that the underlying rapid process can be reversed by carbon dioxide saturated atmospheres. For the first time, we also confirm that the conformational state of ectoine in aqueous bulk solution coincides with crystalline ectoine in its dihydrate state, thereby highlighting the important role of a few bound water molecules.     

The value of single-shot black-blood MR imaging for mapping of the coronary arteries: a comparison of four different orientations during breath-holding and free breathing

The value of ECG-gated single-shot black-blood MR imaging for rapid visualization of the origin and course of the coronary arteries was investigated. The study population included 28 patients with known or suspected cardiac disease. ECG-gated single-shot black-blood MR acquisitions were acquired in the transverse, coronal, sagittal and LAO orientations, during free breathing and breath-holding. The origin of the left coronary artery was most frequently visualized in the coronal and LAO orientations and the origin of the right coronary artery was most frequently visualized in the LAO orientation. Overall, no significant difference was found for the visualization of the coronary artery segments and the overall image quality among acquisitions during breath-holding and free breathing. ECG-gated single-shot black-blood MR imaging (HASTE) appears to be a time-efficient and robust method for mapping of the entire coronary artery tree, without the need for breath-holding. The LAO orientation provides the most consistent visualization of the origins and major coronary artery segments.     

The Banzhaf index in complete and incomplete shareholding structures: A new algorithm

In this global world, many firms present a complex shareholding structure with indirect participation, such that it may become difficult to assess a firm’s controllers. Furthermore, if there are numerous dominant shareholders, the control can be shared between them. Determining who has the most influence is often a difficult task. To measure this influence, game theory allows the modeling of voting games and the computing of the Banzhaf index. This paper firstly offers a new algorithm to compute this index in all structures and then suggests some modelisations of the floating shareholder. Then, our model is applied to a real case study: the French group Lafarge. This exemplary case demonstrates how the float’s structure and hidden coalition can impact the power relationship between dominant shareholders.     

Vehicle routing problems with time windows and multiple service workers: a systematic comparison between ACO and GRASP

This paper systematically compares an ant colony optimization (ACO) and a greedy randomized adaptive search procedure (GRASP) metaheuristic. Both are used to solve the vehicle routing problem with time windows and multiple service workers. In order to keep the results comparable, the same route construction heuristic and local search procedures are used. It is shown that ACO clearly outperforms GRASP in the problem under study. Additionally, new globally best results for the used benchmark problems are presented.     

T1Effects in Sequential Dynamic Susceptibility Contrast Experiments

Residual effects of an initial bolus of gadolinium contrast agent have been previously demonstrated in sequential dynamic susceptibility contrast MR experiments. While these residual effects quickly reach a saturation steady state, their etiology is uncertain, and they can lead to spurious estimates of hemodynamic parameters in activation experiments. The possible influence ofT₁effects is now investigated with experiments in whichT₁weighting is varied as well as with serial regionalT₁measurements. Little evidence for significant residualT₁effects is found, suggesting instead that susceptibility effects underlie these observations. An initial saturation dose of contrast agent minimizes this effect.     

Stochastic production frontiers and panel data: A latent variable framework

This paper deals with the issue of estimating production frontier and measuring efficiency from a panel data set. First, it proposes an alternate method for the estimation of a production frontier on a short panel data set. The method is based on the so-called mean-and-covariance structure analysis which is closely related to the generalized method of moments. One advantage of the method is that it allows us to investigate the presence of correlations between individual effects and exogenous variables without the requirement of some available instruments uncorrelated with the individual effects as in instrumental variable estimation. Another advantage is that the method is well suited to a panel data set with a short number of periods. Second, the paper considers the question of recovering individual efficiency levels from the estimates obtained from the mean-and-covariance structure analysis. Since individual effects are here viewed as latent variables, they can be estimated as factor scores, i.e., weighted sums of the observed variables. We illustrate the proposed methods with the estimation of a stochastic production frontier on a short panel data of French fruit growers.     

Hilbert Functions, Residual Intersections, and Residually S2 Ideals

Let R be a homogeneous ring over an infinite field, IR a homogeneous ideal, and I an ideal generated by s forms of degrees d ₁,...,d _s so that codim( :I)s. We give broad conditions for when the Hilbert function of R/ or of R/( :I) is determined by I and the degrees d ₁,...,d _s . These conditions are expressed in terms of residual intersections of I, culminating in the notion of residually S ₂ ideals. We prove that the residually S ₂ property is implied by the vanishing of certain Ext modules and deduce that generic projections tend to produce ideals with this property.