Ultrasound-assisted efficient synthesis of polyfunctional 1,2,4-triazoles as novel antibacterial and antioxidant agents

Novel 4,5-diphenyl-1H-imidazol-1-yl-1H-1,2,4-triazole derivatives were synthesized using a facile and highly efficient, one-pot, four-component procedure in the presence of KHSO₄ as an acidic catalyst under ultrasonic irradiations in short reaction times (10–25 min) and good to excellent yields (70–96%). The synthesized compounds were screened for their antibacterial activities against Gram-negative (Escherichia coli, Pseudomonas aeruginosa) and Gram-positive (Bacillus subtilis, Micrococcus luteus) bacteria, and their antioxidant activity was also evaluated. Some of the products showed potential antibacterial and high antioxidant activities.     

Different types of electrospun nanofibers and their effect on microfluidic‐based immunoassay

Protein capturing on polymeric substrate of microfluidic devices is a key factor for the fabrication of immunoassay with high sensitivity. In this work, simple and versatile technique of electrospinning was used to produce electrospun nanofibrous membranes (e.NFMs) with high surface area as a substrate for microfluidic‐based immunoassay to increase sensitivity. It was found that the simultaneous use of e.NFM and 1‐Ethylethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide/N‐Hydroxysuccinimide hydroxysuccinimide as coupling agent has synergic effect on antigen immobilization onto the microchannels. It was found that the oxygen plasma technique for the creation of oxygen containing functional group like carboxyl and hydroxyl causes extreme leakage of solution through the microchannels. Thus, due to capillary effect, it is impossible to use hydrophilic substrate to modify microchannels. In order to compensate this problem, it is propose to utilize other type of polymer for the fabrication of nanofiber to answer this important question that if it is possible to enhance the sensitivity of immunoassay just by changing the polymer type? For this purpose, four different polymers, namely, polycaprolactone, poly lactic‐co‐glycolic acid, poly L‐lactic acid, and polyethersolfone were used as the based material for e.NFM fabrication. Results showed that compared with plain poly (dimethylsiloxane) surface of microchannels, poly lactic‐co‐glycolic acidand poly L‐lactic acid, which inherently contain end‐group of carboxyl in their chemical structure, can improve the protein immobilization, which leads to immunoassay signal enhancement through 1‐ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide/N‐hydroxysuccinimide coupling chemistry, significantly.     

A green and practical method for the synthesis of novel pyrano[2,3-<Emphasis Type="Italic">c</Emphasis>]pyrazoles and <Emphasis Type="Italic">bis</Emphasis>-pyrano[2,3-<Emphasis Type="Italic">c</Emphasis>]pyrazoles using sulfonic acid-functionalized ionic liquid

An efficient and practical protocol was developed for the synthesis of novel pyrano[2,3-c]pyrazoles and bis-pyrano[2,3-c]pyrazoles by one-pot four-component reaction of arylaldehyde, ethyl acetoacetate, hydrazine hydrate and dimethyl malonate using SO₃H-functionalized ionic liquid [DMBSI]HSO₄ as a potential green catalyst under solvent-free condition. The method presented is mild, environmentally friendly, inexpensive and functionality tolerant and produces the desired pyranopyrazoles in short reaction times (17–24 min) and excellent yields (85–95%). In addition, the catalyst can be reused at least ten times without almost any change in its catalytic activity.     

An efficient enzymatic method for the separation of stereoisomeric cis and trans-glycidic esters synthesised via Darzen's condensation reactions

Pig's liver esterase (PLE) was used efficiently in phosphate buffer for the separation of stereoisomeric mixtures of cis/trans-ethyl arylglycidates, produced via Darzen's condensation reactions.     

On Anti-Hermitian Metric Connections Preserving a Bicomplex Structure

We give a characterization of bicomplex-holomorphic anti-Hermitian manifolds by using pure metric connection     

On lower bounds for the chromatic number in terms of vertex degree

In this paper we discuss the existence of lower bounds for the chromatic number of graphs in terms of the average degree or the coloring number of graphs. We obtain a lower bound for the chromatic number of K_1,t-free graphs in terms of the maximum degree and show that the bound is tight. For any tree T, we obtain a lower bound for the chromatic number of any K_2,t-free and T-free graph in terms of its average degree. This answers affirmatively a modified version of Problem 4.3 in [T.R. Jensen, B. Toft, Graph Coloring Problems, Wiley, New York, 1995]. More generally, we discuss δ-bounded families of graphs and then we obtain a necessary and sufficient condition for a family of graphs to be a δ-bounded family in terms of its induced bipartite Turán number. Our last bound is in terms of forbidden induced even cycles in graphs; it extends a result in [S.E. Markossian, G.S. Gasparian, B.A. Reed, β-perfect graphs, J. Combin. Theory Ser. B 67 (1996) 1–11].