全文获取类型
收费全文 | 124篇 |
免费 | 16篇 |
国内免费 | 29篇 |
专业分类
化学 | 69篇 |
晶体学 | 1篇 |
力学 | 10篇 |
综合类 | 4篇 |
数学 | 42篇 |
物理学 | 43篇 |
出版年
2023年 | 1篇 |
2022年 | 5篇 |
2021年 | 5篇 |
2020年 | 1篇 |
2019年 | 8篇 |
2018年 | 1篇 |
2017年 | 4篇 |
2016年 | 2篇 |
2015年 | 4篇 |
2014年 | 7篇 |
2013年 | 8篇 |
2012年 | 9篇 |
2011年 | 11篇 |
2010年 | 9篇 |
2009年 | 5篇 |
2008年 | 11篇 |
2007年 | 9篇 |
2006年 | 6篇 |
2005年 | 7篇 |
2004年 | 2篇 |
2003年 | 2篇 |
2002年 | 4篇 |
2001年 | 6篇 |
2000年 | 7篇 |
1999年 | 2篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1990年 | 3篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1974年 | 2篇 |
排序方式: 共有169条查询结果,搜索用时 15 毫秒
31.
32.
An improved gel-based DNA microarray method for detecting single nucleotide mismatch 总被引:5,自引:0,他引:5
Xiao PF Cheng L Wan Y Sun BL Chen ZZ Zhang SY Zhang CZ Zhou GH Lu ZH 《Electrophoresis》2006,27(19):3904-3915
3-D polyacrylamide gel-based DNA microarray platforms provide a high capacity for nucleic acids immobilization and a solution-mimicking environment for hybridization. However, several technological bottlenecks still remain in these platforms, such as difficult microarray preparation and high fluorescent background, which limit their application. In this study, two new approaches have been developed to improve the convenience in microarray preparation and to reduce the background after hybridization. To control the polymerization process, solutions containing acrylamide-modified oligonucleotide, acrylamide, glycerol and ammonium persulfate are spotted onto a functionalized glass slide, and then the slide is transferred to a vacuum chamber with TEMED, so that TEMED is vaporized and diffused into the spots to induce polymerization. By applying an electric field across a hybridized microarray to remove the nonspecifically bound labeled targets, this approach can solve the problem of high fluorescent background of the gel-based microarray after hybridization. Experimental results show that our immobilization method can be used to construct high quality microarrays and exhibits good reproducibility. Moreover, the polymerization is not affected by PCR medium, so that PCR products can be used for microarray construction without being treated by commercial purification cartridges. Electrophoresis can improve the signal-to-noise significantly and has the ability to differentiate single nucleotide variation between two homozygotes and a heterozygote. Our results demonstrated that this is a reliable novel method for high-throughput mutation analysis and disease diagnosis. 相似文献
33.
An inequality model for solving interval dynamic response of structures with uncertain-but-bounded parameters 总被引:1,自引:0,他引:1
In this paper, we look into the dynamic response of structures with uncertain-but-bounded parameters. A new inequality model for determining the interval dynamic response is presented. First we propose an interval dynamic response solution theorem. An inequality model which is a mathematics programming problem is suggested by the presented theorem. Using the central difference method, we substitute the differential items of the inequality model by difference items. By solving them, the interval dynamic response can be obtained. Two examples are used to illustrate the feasibility and the efficiency of the model. 相似文献
34.
Zhi‐Feng Li Ping Wang Qian Zhang Zao‐Ming Chen Chun‐Xiang Wang 《Acta Crystallographica. Section C, Structural Chemistry》2007,63(6):o369-o370
In the title compound, C23H19N5O6·H2O, the two components are linked into complex chains by a combination of two independent O—H...O and two independent N—H...O hydrogen bonds. The complex chains are linked into a two‐dimensional sheet network viaπ–π stacking interactions and C—H...O hydrogen bonds. 相似文献
35.
一类离散分布参数的经验Bayes估计的收敛速度 总被引:35,自引:1,他引:34
In this paper we consider a family of discrete distributions fθ(x)dμ(x), and suppose that the Bayes estimate of φ(θ) with respect to the priori distribution H∈H has a form dH(x) =(?)ak(x)f(x+k)/f(x). where f(x)=∫fθdH(θ) . we construct asequence of empirical Bayes estimates and establish its rate of convergence, and prove that under suitable conditions this rate of convergence can arbitrarily close to 1. we also give a counter-example to the main Theorem 2.1 of [5], and then declare that the "Theorem" does not hold. 相似文献
36.
Di Zao Li Yan Li Xiao Guang Chen Chen Gen Zhu Jing Yang Hong Yan Liu Xian Dao Pan 《中国化学快报》2007,18(11):1335-1338
A series of heterocyclic acid esters of camptothecins as new compounds had been synthesized by acylation method,their in vitro and in vivo antitumor activities were evaluated.The cytotoxic results showed that 6 possessed the best efficacy on six human cancer cell lines in the six classes of CPTs' derivatives.In vivo testing results indicated that 9 had better antitumor activity against mouse liver carcinoma H_(22) than topotecan. 相似文献
37.
Xiaojia Li Peter Schramel Zao Wang Peter Grill Ayfer Yediler Antonius Kettrup 《Mikrochimica acta》1996,124(3-4):171-178
A preconcentration method based on third phase extraction has been developed and combined with inductively coupled plasma atomic emission spectrometry (ICP-AES) for determination of Sb, Pb, Tl at trace levels in water samples. The reagents diantipyrylmethane and potassium iodide were applied for complexing Sb, Pb, Tl and producing a third phase extraction system. This third phase system produces relatively large enrichment factors and complete isolation of trace elements from high salt-containing matrices which influence strongly the plasma condition. Experimental parameters of the extraction, such as concentrations of complexing reagents, pH and extraction time have been optimized. Under the selected conditions, this third phase ICP-AES combination procedure gave multielemental detection limits for Sb, Pb, Tl which are superior by an order of magnitude or more to those obtained by ICP-AES alone without preconcentration. Precision of the technique is better than 10% at the 10 g/l level. The accuracy of this approach is demonstrated by its application to tapwater, artificial seawater and reference seawater samples. 相似文献
38.
39.
锂离子电池正极材料正在向着高比能量、长寿命、低成本、环境友好的方向发展,而具有橄榄石结构的LiFePO4正极材料以其结构稳定、成本低、无污染等优点成为21世纪最理想的绿色电源,但自身也存在缺点。综述了锂离子电池正极材料LiFePO4的研究进展。系统地阐述了LiFePO4的晶体结构特征及性能、多种合成方法以及掺杂多种导电材料和控制晶体生长制备纳米粉体对材料性能的影响。对该材料的应用前景进行了展望,并提出了下一步可能的研究趋势。 相似文献
40.