66.
We present an immunoassay microarray flow-through system for the surface-enhanced Raman scattering (SERS) analysis of bacteria.
The system has been constructed to support and automatize the nondestructive in situ analysis of different microorganisms
in aqueous environment. After the immobilization of the desired antibodies to an activated PEG-coated surface, the chip is
placed into the flow cell which is then flushed with the contaminated sample. Finally, colloidal metal nanoparticles are added
and the cells are detected label-free by SERS. Here, we introduce the successful imaging of single microorganisms in the flow
cell as well as the quantification of microorganisms in water by SERS mapping with a linear range between 4.3 × 10
3 to 4.3 × 10
5 cells/mL. The method has potential for routine application, e.g. for drinking water control.
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