Das “Prinzip des fixierten Partners’ als Werkzeug auf dem Gebiet der Molekularbiologie

Zusammenfassung Das Prinzip des fixierten Partners, wobei eines der aufeinander einwirkenden Objekte in Form einer heterogenen, festen Phase, an Träger gebunden vorliegt, bietet bedeutende Vorzüge beim Isolieren, Nachweis oder anderen Untersuchungen biologischer Objekte. Es wird die Anwendung dieses Prinzips auf den Gebieten der Isolierung von Fermenten, Untersuchung der Codon—Anticodon-Beziehungen, Studium immunologischer Fragen beschrieben.

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The principle of fixed partner as experimental tool in molecular biology research

The high selectivity obtained in systems where one of the interacting partners is present in a fixed state, as solid phase, is demonstrated for three cases: isolation of individual enzymes from a mixture of closely related; specific removal of one kind oft-RNA by a corresponding synthetic template; extraction of the specific antibody by a genetically defined (allotypical) immunoglobulin.

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Herrn Prof. Dr.E. Broda zum 60. Geburtstag gewidmet.     

Quark masses and specific scaling in deep-inelastic processes

Within an operator-product expansion, the problem of the quark-mass dependence of the heavy-flavor contribution to the structure function F ₂ in the current-fragmentation region is considered at high values of the momentum transfer squared Q ². A linear combination of the structure functions F ₂ ^c and F ₂ is found that possesses a specific scaling property for Q ² → ∞ at small fixed values of the Bjorken variable x. A lower bound on the ratio F ₂ ^c /F ₂ is calculated and is compared with data obtained at the HERA collider.     

RS model with a small curvature and dielectron production at the LHC

In the framework of the Randall-Sundrum-like scenario with the small curvature κ (RSSC model), p _⊥-distributions for the dielectron production at the LHC are calculated. For the summary statistics taken at 7 TeV (L = 5 fb^?1) and 8 TeV (L = 20 fb^?1), the exclusion limit on the 5-dimensional gravity scale M ₅ is found to be 6.35 TeV at 95% C.L. For √s = 13 TeV and integrated luminosity 30 fb^?1, the LHC search limit is estimated to be 8.95 TeV. These limits on M ₅ are independent of κ, provided the relation κ ? M ₅ is satisfied.     

Proteasome inhibitors: an expanding army attacking a unique target

Proteasomes are large, multisubunit proteolytic complexes presenting multiple targets for therapeutic intervention. The 26S proteasome consists of a 20S proteolytic core and one or two 19S regulatory particles. The 20S core contains three types of active sites. Many structurally diverse inhibitors of these active sites, both natural product and synthetic, have been discovered in the last two decades. One, bortezomib, is used clinically for treatment of multiple myeloma, mantle cell lymphoma, and acute allograft rejection. Five more recently developed proteasome inhibitors are in trials for treatment of myeloma and other cancers. Proteasome inhibitors also have activity in animal models of autoimmune and inflammatory diseases, reperfusion injury, promote bone and hair growth, and can potentially be used as anti-infectives. In addition, inhibitors of ATPases and deubiquitinases of 19S regulatory particles have been discovered in the last decade.     

Discovery of a potent and highly β1 specific proteasome inhibitor from a focused library of urea-containing peptide vinyl sulfones and peptide epoxyketones

Syringolins, a class of natural products, potently and selectively inhibit the proteasome and show promising antitumour activity. To gain insight in the mode of action of syringolins, the ureido structural element present in syringolins is incorporated in oligopeptide vinyl sulfones and peptide epoxyketones yielding a focused library of potent new proteasome inhibitors. The distance of the ureido linkage with respect to the electrophilic trap strongly influences subunit selectivity within the proteasome. Compounds 13 and 15 are β5 selective and their potency exceeds that of syringolin A. In contrast, 5 may well be the most potent β1 selective compound active in living cells reported to date.     

Measurement of the polarization correlation parameter C nn in the pp elastic scattering with an unpolarized proton target and an unpolarized 1-GeV proton beam

The polarization correlation parameter C _nn in the elastic proton-proton scattering was for the first time measured with a high statistical accuracy at 1 GeV using an unpolarized proton beam incident on an unpolarized proton target. Both outgoing protons from the reaction were detected in coincidence by means of a two-arm focusing magnetic spectrometer installed at nonsymmetric angles corresponding to the center-of-mass scattering angle Θ_cm = 62.25°. The correlation parameter C _nn , as well as polarizations P ₁ and P ₂ of the secondary protons, were measured by means of multiwire proportional chamber polarimeters placed at the focal planes of each spectrometer arm. The obtained data are compared with the predictions of the phase-shift analysis. The connection with the experiment on C _nn and polarization measurements in the (p, 2p) reaction on ⁴He and other light nuclei in similar kinematics is discussed     

Die Natur der OberflÄche und die Adsorptionseigenschaften von Siliziumdioxid

Zusammenfassung Auf Grund der durchgeführten Untersuchungen des Hydratationsgrades der Oberfl?che der α-Quarzproben und Silikagele verschiedener Dispersit?t, sowie der Adsorptionsmessungen, der Messungen der differentiellen Absorptionsw?rme und der Kernresonanz-Spektren wurde der Aufbau der Hydratschicht von SiO₂ und der Wechselwirkungsmechanismus der Wassermoleküle mit der Oberfl?che untersucht. Es wurde die Abh?ngigkeit der spezifischen Adsorptionsaktivit?t der Oberfl?che von der Bearbeitungstemperatur der SiO₂-Proben und ihrer Dispersit?t bestimmt. Herrn Prof. Dr. O. Glemser, Direktor des Anorganisch-Chemischen Institutes der Universit?t G?ttingen, Dr. Schwarzmann, Dr. Freund von diesem Institut und auch Dr. St?ber von der Medizinischen Forschungsanstalt der Max-Planck-Gesellschaft danken wir sehr für anregende Diskussionen und sehr wertvolle Bemerkungen.     

Study of nuclear effects in nucleon structure functions up to and beyond the kinematic limit

An experiment on deep inelastic muon nucleon scattering in order to study the x-dependence of nucleon structure functions and their ratios over a range ofQ ²=50–200 GeV² andx= =0·4–2·0 is proposed. Such measurements performed on a number of nuclei with atomic weights from 2 to 207 provide new information for detailed studies of the EMC effect and test models explaining thex-behaviour of the nucleon structure functions and their ratios. The measurements are decisive for the proof that a quark-parton model of the nucleus describes adequately nuclear structure probed at high energies. The upgraded high luminosity BCDMS spectrometer with improved resolution in transferred energy can be used for the measurements.Dedicated to the 30th anniversary of the Joint Institute for Nuclear Research.     

Study of Inelastic <Emphasis Type="Italic">A</Emphasis>(<Emphasis Type="Italic">p,p′</Emphasis>)<Emphasis Type="Italic">X</Emphasis> Reaction with Nuclei at 1 GeV

The secondary-proton polarization and differential cross sections for the (p, p') inelastic reaction on ²⁸Si and ⁵⁶Fe nuclei at the initial proton energy of 1 GeV were measured over a wide range of the scattered-proton momenta at a laboratory angle of Θ = 21?. Scattered protons were detected by means of a magnetic spectrometer equipped with a polarimeter based on multiwire proportional chambers and a carbon analyzer. A structure in the polarization and cross section data, which is probably related to quasielastic scattering off nucleon correlations in the ²⁸Si and ⁵⁶Fe nuclei, was observed as earlier in the analogous data for ¹²C and ⁴⁰Ca nuclei. Momentum intervals within which cross-section ratios for nuclei did not depend on the scattered-proton momentum were found.