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11.
Sirous Ghobadi Fakhroddin Yousefi Fatemeh Khademi Samira Padidar Ali Mostafaie 《Journal of separation science》2012,35(21):2827-2833
Plant nonspecific lipid transfer proteins are small basic proteins that transport phospholipids between membranes and are subdivided into two subfamilies, nsLTP1 (9 kDa) and nsLTP2 (7 kDa). LTPs have potential application in the defense reactions against pathogens and the drug delivery systems. Many efforts have been made for purification of different nsLTPs from various plants; however, most of them used successive purification procedures. We have developed a relatively simple and efficient method for the purification of rice nsLTP1, based on the proteolytic activity of kiwifruit actinidin on the rice seed extract and one‐step chromatographic procedure on a CM‐Sepharose column. The purity of protein was determined by reversed‐phase high‐performance liquid chromatography (RP‐HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE). The isolated LTP1 migrated as a homogenous polypeptide with molecular mass of 9 kDa that confirms the efficiency of actinidin on the digestion of major contaminations present in the rice seed extract without any harmful effect on the LTP1. The advantages of using proteolytic activity of actinidin in purifying rice LTP1 includes the reduced separation time allowing the purification of LTP1 in one‐step chromatographic procedure, low costing, high efficiency, and the relative simplicity of the method. 相似文献
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Kavosh Majlesi Clemente Bretti Rosalia Maria Cigala Concetta De Stefano Kimia Majlesi Silvio Sammartano 《Journal of solution chemistry》2018,47(3):528-543
A potentiometric method has been used for the determination of the protonation constants of N-(2-hydroxyethyl)iminodiacetic acid (HEIDA or L) at various temperatures 283.15?≤?T/K?≤?383.15 and different ionic strengths of NaCl(aq), 0.12?≤?I/mol·kg?1?≤?4.84. Ionic strength dependence parameters were calculated using a Debye–Hückel type equation, Specific Ion Interaction Theory and Pitzer equations. Protonation constants at infinite dilution calculated by the SIT model are \( \log_{10} \left( {{}^{T}K_{1}^{\text{H}} } \right) = 8.998 \pm 0.008 \) (amino group), \( \log_{10} \left( {{}^{T}K_{2}^{\text{H}} } \right) = 2.515 \pm 0.009 \) and \( \log_{10} \left( {{}^{T}K_{3}^{\text{H}} } \right) = 1.06 \pm 0.002 \) (carboxylic groups). The formation constants of HEIDA complexes with sodium, calcium and magnesium were determined. In the first case, the formation of a weak complex species, NaL, was found and the stability constant value at infinite dilution is log10KNaL?=?0.78?±?0.23. For Ca2+ and Mg2+, the CaL, CaHL, CaL2 and MgL species were found, respectively. The calculated stability constants for the calcium complexes at T?=?298.15 K and I?=?0.150 mol·dm?3 are: log10βCaL?=?4.92?±?0.01, log10βCaHL?=?11.11?±?0.02 and \( \log_{10} \beta_{\text{Ca{L}}_{2}} \)?=?7.84?±?0.03, while for the magnesium complex (at I?=?0.176 mol·dm?3): log10βMgL?=?2.928?±?0.006. Protonation thermodynamic functions have also been calculated and interpreted. 相似文献
13.
The recently proposed systems of various anions (A) confined inside C60 , A− @ C60 , which in turn behave as large and stable anions, (A @ C60)− , can find potential applications in various fields. On the other hand, it has earlier been shown that from the dihalogens (X2 ) encapsulated C60 , X2 @ C60 , only F2 @ C60 can be introduced as a system in which the cage acts as a cation C60+ and interacts with an endohedral anion, F2− , forming the F2− @ C60+ as a single-molecule crystal compound. In this work, two density functional theory energy decomposition analysis (EDA) schemes, where in one of them the noninteracting kinetic, electrostatic, and exchange-correlation energies come into play while another scheme, called as EDA-SBL, includes the steric, electrostatic, and quantum effects as essential ingredients (S. Liu, J. Chem. Phys. 2007 , 126, 244103), are utilized to find out what energetic components govern the unique characteristics of the (A @ C60)− and X2 @ C60 confinements. It is shown that the noninteracting kinetic energy and steric energies have important contributions to the total interaction energies for the considered systems. However, there are other confinements for which the electrostatic and exchange-correlation contributions play also imperative roles. Furthermore, we find reasonable correlations between interaction energies and their components as well as the energetic components themselves, leading to an alternative EDA scheme including the noninteracting kinetic, steric, and electrostatic energies for investigations on other endohedral fullerenes. Extending our analyses to large size confinements, Cl− @ Cn with n up to 90 as illustrative examples, the quantitative cooperativity concept is also explored, where the positive and negative cooperativity profiles unveil a specific size of the anionic confinements to form the most stable large anion. 相似文献
14.
This paper deals with the inverse Data Envelopment Analysis (DEA) under inter-temporal dependence assumption. Both problems, input-estimation and output-estimation, are investigated. Necessary and sufficient conditions for input/output estimation are established utilizing Pareto and weak Pareto solutions of linear multiple-objective programming problems. Furthermore, in this paper we introduce a new optimality notion for multiple-objective programming problems, periodic weak Pareto optimality. These solutions are used in inverse DEA, and it is shown that these can be characterized by a simple modification in weighted sum scalarization tool. 相似文献
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In this paper, a novel optical nanoantenna based on two-dimensional plasmonic nano-ring antenna array is proposed, investigated numerically and compared with a nano-disk antenna array. The results show that the nano-ring structure has resonances similar to the resonances of nano-disk structure but in higher wavelengths. These resonances are tunable by varying the structural parameters of the ring. The optical properties of the proposed structure are investigated in the context of designing a plasmonic refractive index sensor. The nano-ring antenna array sensor has large sensitivity in comparison to the conventional nano-disk antenna arrays. The special feature of the presented structure and the device concepts introduced in this work are applicable in realization of various integrated components and could play an important role in development of plasmonic sensors. 相似文献
18.
T. Gamze Ulusoy Ghobadi Amir Ghobadi Muhammed Buyuktemiz Elif Akhuseyin Yildiz Dilara Berna Yildiz H. Gul Yaglioglu Yavuz Dede Ekmel Ozbay Ferdi Karadas 《Angewandte Chemie (International ed. in English)》2020,59(10):4082-4090
Herein, we establish a simple synthetic strategy affording a heterogeneous, precious metal‐free, dye‐sensitized photoelectrode for water oxidation, which incorporates a Prussian blue (PB) structure for the sensitization of TiO2 and water oxidation catalysis. Our approach involves the use of a Fe(CN)5 bridging group not only as a cyanide precursor for the formation of a PB‐type structure but also as an electron shuttle between an organic chromophore and the catalytic center. The resulting hetero‐functional PB‐modified TiO2 electrode demonstrates a low‐cost and easy‐to‐construct photoanode, which exhibits favorable electron transfers with a remarkable excited state lifetime on the order of nanoseconds and an extended light absorption capacity of up to 500 nm. Our approach paves the way for a new family of precious metal‐free robust dye‐sensitized photoelectrodes for water oxidation, in which a variety of common organic chromophores can be employed in conjunction with CoFe PB structures. 相似文献
19.
This report presents the use of 2-DE with ultrasensitive fluorescence detection as a chemical cytometry tool to characterize the protein and biogenic amine content of single cells from the RAW 264.7 murine macrophage cell line. Cells were sorted by cell cycle prior to 2-DE analysis. Cells in the G2/M phase of the cell cycle were aspirated into the first-dimensional capillary and lysed. The cellular contents were fluorescently labeled and first separated by capillary sieving electrophoresis (CSE). Over 380 fractions were transferred from the first-dimensional capillary to the second-dimensional capillary, where components were further separated by MEKC and detected by laser-induced fluorescence. Twenty-five spots common to the four electropherograms were fit with a 2-D Gaussian surface to determine spot position, width, and amplitude. The RSD in CSE mobility was 1.0 +/- 0.6%. The mean uncertainty in spot position was 1.3 times larger than the mean spot width in the CSE dimension. The average SD in MEKC migration time was 0.37 +/- 0.13 s, which is smaller than the average spot size in this dimension. Spot capacity was 200. The RSD in spot amplitude was 50%, reflecting a large cell-to-cell variation in component expression. 相似文献
20.
R. Moradian N. Ghobadi M. Roushani M. Shamsipur 《Journal of the Iranian Chemical Society》2011,8(1):S104-S109
Nanocrystals provide a confinement effect for nanoscale engineering. In this work the cadmium selenide quantum dot and its nanoparticle films have been deposited by chemical bath deposition method (CBD). Effects of deposition time, pH and annealing operation on the optical and structural of CdSe nanoparticle film were studied. The energy band gap, structure and morphology of the samples are investigated by X-ray diffraction (XRD), UV-Vis spectrometer and scanning electron microscope (SEM). It was found that the optical band gap, nanoparticle size and thin film configuration are changed by varying pH, deposition time and annealing operation. 相似文献