全文获取类型
收费全文 | 51篇 |
免费 | 1篇 |
国内免费 | 5篇 |
专业分类
化学 | 29篇 |
力学 | 2篇 |
数学 | 12篇 |
物理学 | 14篇 |
出版年
2022年 | 1篇 |
2021年 | 1篇 |
2020年 | 3篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2017年 | 2篇 |
2016年 | 2篇 |
2015年 | 1篇 |
2014年 | 1篇 |
2013年 | 6篇 |
2012年 | 7篇 |
2011年 | 4篇 |
2010年 | 3篇 |
2008年 | 1篇 |
2007年 | 3篇 |
2006年 | 2篇 |
2005年 | 2篇 |
2003年 | 2篇 |
2002年 | 5篇 |
2001年 | 1篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1991年 | 1篇 |
1989年 | 2篇 |
排序方式: 共有57条查询结果,搜索用时 31 毫秒
11.
Numerical results are reported for the computation of periodicsolution paths for a suspension bridge model represented bythe equation. un + EIuxxxx + ut, + Ku+ = W(x) + sin x sin µt. with hinged-end boundary conditions, as the forcing amplitude and frequency µ are varied. The term Ku+ models the factthat there is restoring force due to the cables only when theyare being stretched. It is found that an S-shaped curve is obtainedwhen the displacement amplitudes are plotted against the forcingamplitudes for some frequency regimes. As a two-parameter problem,it appears that the solution set resembles a cusp-like surfacewith the singular point near linear resonance. While the effectof strengthening the cable (i.e. increasing K) will enhancethe occurrence of the multiple solutions, the effect of increasingthe damping coefficient gives the opposite effect. 相似文献
12.
Rapid Preparation of Monolithic Columns for Capillary Electrochromatography Separation 总被引:1,自引:0,他引:1
Wen Jun GONG Yi Jun ZHANG Yu Ping ZHANG Seong Ho CHOI 《中国化学快报》2006,17(6):813-816
Sol-gel technology can provide a versatile approach to the synthesis of organic polymers and organic-inorganic hybrid materials applied in HPLC, micro-HPLC and capillary electrochromatography (CEC)1. It can take place under extraordinarily mild thermal co… 相似文献
13.
We experimentally attempted to understand the vibration characteristics of a flexible pipe excited by vortex shedding. This has been extensively studied in the previous decades (for example, see Sarpkaya 1979 Journal of Applied Mechanics46, 241-258; Price et al. 1989 Eighth International Conference on Offshore Mechanics and Arctic Engineering, The Hague-March 19 -23, 447-454; Yoerger et al. 1991 Journal of Offshore Mechanics and Arctic Engineering, Transaction of Engineers113, 117-127; Grosenbaugh et al. 1991Journal of Offshore Mechanics and Arctic Engineering, Transaction of Engineers113 , 199-204; Brika and Laneville 1992 Journal of Fluid Mechanics250, 481-508; Chakrabarti et al. 1993 Ocean Engineering20, 135-162; Jong 1983 Ph.D. Dissertation, Department of Ocean Engineering, M. I. T.; Kimet al. 1986 Journal of Energy Resources Technology, Transactions of American Society of Mechanical Engineers108, 77-83). However, there are still areas that need more study. One of them is the relation between spatial characteristics of a flow-induced vibrating pipe, such as its length, the distribution of wave number, and frequency responses. A non-linear mechanism between the responses of in-line and cross-flow directions is also an area of interest, if the pipe is relatively long so that structural modal density is reasonably high. In order to investigate such areas, two kinds of instrumented pipe were designed. The instrumented pipes, of which the lengths are equally 6 m, are wound with rubber and silicon tape in different ways, having different vortex-shedding conditions. One has uniform cross-section of diameter of 26·7 mm, and the other has equally spaced four sub-sections, which are composed of different diameters of 75·9, 61·1, 45·6 and 26·7 mm. Both pipes are towed in a water tank (200 m×16 m×7 m) so that they experienced different vortex-shedding excitations. Various measures were obtained from the towing experiment, including frequency responses, the time-domain tracing of in-line and cross-flow responses, and Wigner-Ville distributions. The experimental results analyzed by using these measures exhibit several valuable features. One of them is that the natural frequencies and their corresponding strain mode shapes dominate the strain response of the uniform pipe. However for those of non-uniform pipe, the responses are more likely local and many modes participate in it. 相似文献
14.
Recently, it was shown that the absence of circular polarization of visible light from quasars severely constrains the interpretation of axion-like particles (ALPs) as a solution for the generation of linear polarization. Furthermore, the new observation of linear polarization in radio wavelength from quasars, similar to the earlier observation performed in the optical bands, makes the ALPs scenario inconsistent with at least one of the two observations. In this study, we extend this scenario by including more scalars. We find that the effects from scalar and pseudoscalar neutralize each other, thereby suppressing the circular polarization, while preserving consistent linear polarization, as observed in both the visible and radio wave bands. 相似文献
15.
Changhun Park Seokhun Yun Sang Yup Lee Kyungmoon Park Jinwon Lee 《Applied biochemistry and biotechnology》2012,167(3):425-438
The global pool of intracellular metabolites is a reflection of all the metabolic functions of an organism. In the absence of in situ methods capable of directly measuring metabolite pools, intracellular metabolite measurements need to be performed after an extraction procedure. In this study, we evaluated the optimization of technologies for generation of a global metabolomics profile for intracellular metabolites in Klebsiella oxytoca. Intracellular metabolites of K. oxytoca were extracted at the early stationary phase using six different common extraction procedures, including cold methanol, boiling ethanol, methanol/chloroform combinations, hot water, potassium hydroxide, and perchloric acid. The metabolites were subsequently collected for further analysis, and intracellular metabolite concentration profiles were generated using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry. During analysis, the stability of metabolites extracted using cold methanol was clearly higher than that obtained by other extraction methods. For the majority of metabolites, extracts generated in this manner exhibited the greatest recovery, with high reproducibility. Therefore, the use of cold ethanol was the best extraction method for attaining a metabolic profile. However, in another parallel extraction method, perchloric acid may also be required to maximize the range of metabolites recovered, particularly to extract glucose 1-phosphate and NADPH. 相似文献
16.
Soohyun Park Seung Pil Pack Jinwon Lee 《Applied biochemistry and biotechnology》2012,167(7):1845-1853
We examined the expression of the phosphoenolpyruvate carboxylase (PEPC) gene from marine bacteria in Escherichia coli using codon optimization. The codon-optimized PEPC gene was expressed in the E. coli K-12 strain W3110. SDS-PAGE analysis revealed that the codon-optimized PEPC gene was only expressed in E. coli, and measurement of enzyme activity indicated the highest PEPC activity in the E. coli SGJS112 strain that contained the codon-optimized PEPC gene. In fermentation assays, the E. coli SGJS112 produced the highest yield of oxaloacetate using glucose as the source and produced a 20-times increase in the yield of malate compared to the control. We concluded that the codon optimization enabled E. coli to express the PEPC gene derived from the Glaciecola sp. HTCC2999. Also, the expressed protein exhibited an enzymatic activity similar to that of E. coli PEPC and increased the yield of oxaloacetate and malate in an E. coli system. 相似文献
17.
Byeon IJ Hou G Han Y Suiter CL Ahn J Jung J Byeon CH Gronenborn AM Polenova T 《Journal of the American Chemical Society》2012,134(14):6455-6466
The capsid protein (CA) of human immunodeficiency virus 1 (HIV-1) assembles into a cone-like structure that encloses the viral RNA genome. Interestingly, significant heterogeneity in shape and organization of capsids can be observed in mature HIV-1 virions. In vitro, CA also exhibits structural polymorphism and can assemble into various morphologies, such as cones, tubes, and spheres. Many intermolecular contacts that are critical for CA assembly are formed by its C-terminal domain (CTD), a dimerization domain, which was found to adopt different orientations in several X-ray and NMR structures of the CTD dimer and full-length CA proteins. Tyr145 (Y145), residue two in our CTD construct used for NMR structure determination, but not present in the crystallographic constructs, was found to be crucial for infectivity and engaged in numerous interactions at the CTD dimer interface. Here we investigate the origin of CA structural plasticity using solid-state NMR and solution NMR spectroscopy. In the solid state, the hinge region connecting the NTD and CTD is flexible on the millisecond time scale, as evidenced by the backbone motions of Y145 in CA conical assemblies and in two CTD constructs (137-231 and 142-231), allowing the protein to access multiple conformations essential for pleimorphic capsid assemblies. In solution, the CTD dimer exists as two major conformers, whose relative populations differ for the different CTD constructs. In the longer CTD (144-231) construct that contains the hinge region between the NTD and CTD, the populations of the two conformers are likely determined by the protonation state of the E175 side chain that is located at the dimer interface and within hydrogen-bonding distance of the W184 side chain on the other monomer. At pH 6.5, the major conformer exhibits the same dimer interface as full-length CA. In the short CTD (150-231) construct, no pH-dependent conformational shift is observed. These findings suggest that the presence of structural plasticity at the CTD dimer interface permits pleiotropic HIV-1 capsid assembly, resulting in varied capsid morphologies. 相似文献
18.
19.
Electrolysis and isoelectric focusing 总被引:2,自引:0,他引:2
This paper consists of two parts. In the first part, the authorsprove the existence of steady-state solutions for a three-specieselectrolyte. The species are subject to both dissociation-associationreactions inside the electrolyte and electrochemical reactionsat the boundary electrodes. This is a common occurrence in electrolysis.In the second part, the authors investigate how to use thismodel to describe isoelectric focusing, which is a common techniqueused to separate large protein molecules. In particular, theisoelectric focusing point for a particular type of proteinmolecule is calculated using formal perturbation analysis. 相似文献
20.
INVESTIGATION ON THE WHIRLING MOTION OF FULL ANNULAR ROTOR RUB 总被引:2,自引:0,他引:2
Y.S. CHOI 《Journal of sound and vibration》2002,258(1):191-198